1995). While the basal swimming behavior of worms in the two buffers was indistinguishable (data not shown), we observed a striking difference in behavior in the two buffers when ethanol was added. Figure 5a shows the time course of intoxication in 500 mM ethanol in the two buffers. Swimming movement was quantified as the number of head swings during a 10 second time window. The motion of the animals exposed to ethanol decreased in both conditions by 5 minutes of exposure. However, animals assayed in Dent's buffer became essentially immotile by 10 minutes, whereas animals assayed in NGM buffer decreased motion but remained motile during the entire 20 minutes of treatment.
