In light of differential transcript usage, GO analysis, and Cdc5 requirement, we assessed the detectable expression of FlyBase’s “spliceosome complex” gene list (GO:0005681) (Figure 6A). We predicted that decreasing expression of highly expressed splice factors in the MB neurons would affect alcohol associative memories. We choose candidate targets RNA export and export binding protein 1 (Ref1), Protein on ecdysone puffs (Pep), RNA helicase Rm62 (also referred to as p68), and CG7971, which is orthologous to serine/arginine repetitive matrix 2 (SRRM2) (Figure 6B). Adult MB-specific knockdown of each of these targets impaired ethanol-cue-induced memory without affecting odor sensitivity (Figure 6C, Figures S9 and S10).
