Chunk #9 — MATERIALS AND METHODS — Isolation of lymphocyte DNA

Source

Increased OPRM1 DNA methylation in lymphocytes of methadone-maintained former heroin addicts.

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Text

DNA was extracted from peripheral blood lymphocytes using a salting out procedure or from whole blood using the Gentra Puregene kit (QIAGEN GmbH, Hilden, Germany) according to the manufacturer’s protocol.