least 10 s. Inward EPSCs and outward IPSPs were recorded by filling the patch pipette with a low chloride internal solution (ECl–= −90 mV) and holding the cell at −40 mV, which is near the midpoint between ECl– and EK+/Na+. Notably, the average shape of sIPSCs recorded in hSS displayed a prolonged decay as compared to the average EPSCs recorded from hCS, as is commonly observed in cortical neurons55 (Extended Data Fig. 4b).
