It is worth noting that the genes in the WNT-associated pathways presented a unique pattern. The expression of this group of genes was not uniform, with some genes being upregulated during D0–D7, some genes being upregulated during D7–28, and some genes being down regulated (Table 2). We found that the group of genes that were up regulated during D0–7 and genes that were up regulated during D7–28 did not overlap; however, PRKCA was found to be upregulated during D0–7 and down regulated during D7–28.Table 2WNT pathway enrichment by KEGG.Stage(Day)patterninvolved genes0–7upCXXC4, PPP3R1, NKD2, MAPK10, SIAH1, PRICKLE1, PRICKLE2, WNT10B, CSNK1E, NLK, MAPK9, WNT2B, PLCB1, CTBP2, PRKCB, PLCB4, RAC3, PRKCA, DAAM1, CAMK2G, CAMK2A, CAMK2B, PRKCG, PPP3CA, APC2, MAPK8, PORCN, NOTUM, BTRC, GSK3B, FRAT1, FRAT2, PRKACB, APC, PPP3CB, PPP3CC, WNT7A7–28upNFATC4, FZD5, FZD6, CCND1, SFRP4, JUN, BAMBI, FZD8, WNT8B, WNT5A7–28downPRKCA, NLK, WNT7A
