define the decrease in speed at 10 minutes of treatment, relative to untreated animals of the same genotype, as the initial sensitivity of animals to ethanol. We have previously shown that wild-type worms move faster at 30 minutes relative to their speed at 10 minutes of ethanol exposure, and we consider the amount of recovery of speed between 10 and 30 minutes to be the degree of acute functional tolerance. We judged animals to be defective in AFT if they had a reduced recovery of speed at 30 minutes versus the genetic background strain (npr-1(ky13) or N2).
