The purified LPHN3-OLF domain, FLRT3-LRR and their complex were analyzed by SEC to ensure sample monodispersity for subsequent biophysical experiments and to help interpretation of the crystallography packing data. LPHN3-OLF and the OLF-LRR complex eluted as single peaks, indicating ideal monodispersity and apparent MWs consistent with their monomeric forms (Figure 1C). However, the elution profile of LRR alone appears larger than expected consistent with the fact that FLRT3-LRR undergoes a concentration-dependent oligomerization due to a low affinity homophilic interaction of the LRR domain of FLRT3 (Seiradake et al., 2014).
