response to E2 (Figure 1 and Supplementary Table 3). Gene ontology (GO) analysis using GOstat27 identified a number of significantly enriched GO categories within genes proximal to the identified DMRs (Table 1). Motif enrichment analysis of genomic sequences of the top 100 significant E2 DMRs as well as estrogen receptor-β promoter methylation-correlated regions identified an enrichment for the SP-1 and estrogen receptor transcription factor-binding motifs (Supplementary Figure 2).
