Critically, we observed that BPD and control iPSCs differentiated similarly to NPs and neurons, thereby paving the way to identify DEGs between controls and BPDs at a given stage of differentiation of iPSCs. This is in contrast to Madison et al [22], who observed a deficit in the proliferative characteristics of NPs as well as in neuronal differentiation ability in their BPD lines. Although we presented one iPSC cell line/donor for 4 donors in the current study, we did confirm the NP differentiation capacity of 2 additional lines from each of the 4 BPD donors and 3 lines from 2 additional affected family members (data not shown). Chen et al [21] did not report a lack of differentiation capacity in their BPD iPSCs.
