We compared sequencing depth at protein-coding regions in TOPMed WGS and ExAC WES data. The ExAC WES depth at each sequenced base pair on human genome build GRCh37 was downloaded from the ExAC browser website (http://exac.broadinstitute.org). When sequencing depth summary statistics for a base pair were missing, we assumed depth <10× for this base pair. Only protein-coding genes from consensus coding sequence were analysed and the protein-coding regions (CDS) were extracted from GENCODE v.29. When analysing ExAC sequencing depth, we used GENCODE v.29 lifted to human genome build GRCh37. When comparing sequencing depth for each gene individually in TOPMed and ExAC, we used only genes present in both GRCh38 and GRCh37 versions of GENCODE v.29.
