Western blotting for 5-HT3 receptor protein was performed as described [29]. Briefly, tissues from specific CNS regions were homogenized in protease containing buffer and separated on acrylamide gels. After transfer, membranes were probed using rabbit polyclonal anti-5-HT3 antibody (Abcam, Cambridge, Massachusetts, USA) at 1 : 500 dilution. Membranes were then stripped and reprobed for actin abundance thus allowing normalization.
