kept in the home cage for 8 or 28 days after the final cocaine dose prior to tissue harvest; elevated locomotor responding was maintained for the 28 day withdrawal period (Figure S1). Quantitative polymerase chain reaction (qPCR) validation was performed on RNA samples used for library preparation (8 animals analyzed separately in each treatment group) and on two biological replicate groups of mice sensitized in a similar manner (Kiraly et al., 2010): 8 each saline and cocaine; 4 each saline, cocaine, and 28 days withdrawal. Pairwise comparisons were performed and subjected to t-test (p<0.05 taken as significant).
