Finally, further emphasizing the reciprocally related expression of miR-34a and its putative targets, given the upregulation of miR-34a in the postmortem cerebellum of BD patients, we observed similarly decreased ANK3 and CACNB3 mRNA levels, which were also negatively correlated with miR-34a expression (Figures 2G-J). Of note, the limited amount of RNA collected from iNs did not allow further investigation of the correlation of targets to the level of miR-34a expression. Taken all together, these observations further support the validation of the two miR-34a targets ANK3 and CACNB3.
