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Chunk #9 — METHODS AND MATERIALS — Genotyping

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The influence of GABRA2, childhood trauma, and their interaction on alcohol, heroin, and cocaine dependence.
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A genomic region containing sequence 5 kb upstream and 1 kb downstream of GABRA2 was retrieved from NCBI Human Build 35.1. Haplotype tagging SNPs were identified using a previously described design pipeline (43). Ten GABRA2 SNPs were genotyped using the Illumina GoldenGate platform (43). Rs numbers for the 10 SNPs, the bases for alleles 1 – 2, together with the allele 2 frequencies, are shown in Figure 1. For each SNP, alleles 1 and 2 are located on opposite DNA strands.