Chunk #79 — Materials and Methods — Tissue Culture Procedures and Luciferase Assays

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Essential role of chromatin remodeling protein Bptf in early mouse embryos and embryonic stem cells.

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Growth rates of ES cell lines were obtained on gelatinized plates in the absence of feeder layers. Cells were plated @ 1.0×105 in 6 well plates. Cell number was determined every day for 4 consecutive days with changes in ESC Growth media occurring every day.