Chunk #10 — MATERIALS AND METHODS — Immunocytochemistry

Source

Efficient generation of functional dopaminergic neurons from human induced pluripotent stem cells under defined conditions.

Embedded

yes

Text

MA http://www.millipore.com) 1:800, TH (Pel-freez, Rogers, AR http://www.pelfreez-bio.com P40101) 1:500, TH clone TH-16 (T2928 Sigma) 1:1,000; Girk2 (Alamone) 1:250; Musashi (eBioscience, San Diego, CA http://www.ebioscience.com) 1:50, and as secondary antibodies: Alexa Fluor 488 Goat Anti-Mouse, Alexa Fluor 594 Goat Anti-Mouse, Alexa Fluor 488 Goat Anti-Rabbit, Alexa Fluor 594 Goat Anti-Rabbit. Hoechst 33342 (Molecular Probes H3570) 1:1,000 were used for nuclei identification. Images were captured on a Nikon fluorescence microscope.