Chunk #41 — STAR Methods — Experimental Model and Subject Details — Cell Culture — Differentiation of iPSCs to Hematopoietic Progenitor Cells (iHPCs)

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iPSC-Derived Human Microglia-like Cells to Study Neurological Diseases.

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yes

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iHPC Differentiation Base Medium Formulation: IMDM (50%), F12 (50%), insulin (0.02 mg/ml), holo-transferrin (0.011 mg/ml), sodium selenite (13.4 μg/ml), ethanolamine (4 μg/ml)(can use ITSG-X, 2% v/v, Thermo Fisher Scientific), L-ascorbic acid 2-Phosphate magnesium (64 μg/ml; Sigma), monothioglycerol (400 μM), PVA (10 ng/ml; Sigma), Glutamax (1X), chemically-defined lipid concentrate (1X), non-essential amino acids (NEAA; 1X), Penicillin/Streptomycin (P/S;1%). Use 0.22 μm filter.