with CPA in both the cytokine loci as well as in their transcription factors loci analyzed. Some of these differentially methylated regions found in IL-6, IL-4, IL-10, STAT6 and NFkB were located in known regulatory regions whereas others, to our knowledge, were previously unknown as regulatory areas. However, using the ENCODE database, we were able to identify key regulatory elements in many of these regions that indicate that they might be involved in the regulation of cytokine expression. Further work will be needed to investigate the exact role of these new candidate regulatory regions in the regulation of transcription of these genes and particularly in the context of problems with regulation of physical aggression during childhood.
