of opioid binding and MOR activation. However, exogenous MOR-specific agonists can be supplied to the cultures, and MOR functionality can be directly probed in response to ethanol-mediated facilitation of GABAergic synaptic release. In this study, we observed a more robust inhibition of sIPSC frequency in D40 AD-iNs following acute ethanol application in response to DAMGO (Supplemental Fig. S3). Ultimately, the use of human stem cell-derived iNs yields novel information about human-specific mechanisms of a highly prevalent OPRM1 SNP, A118G, in response to ethanol.
