The generation of hMGEOs and hCOs led us to establish an organoid-based platform to study human interneuron migration. We noticed extensive neuronal migration in hMGEOs embedded inside matrigel droplets, typically with individual neurons migrating to the surface of the embedding matrix (Figure S6A-6D). Similarly, hCOs displayed active neuronal migration inside matrigel (Figure S6E). We then confirmed a robust interneuron migration across organoids by fusing hMGEOs derived from H1 hESCs and NKX2-1GFP/w HES3 cells (Figure S6F). These results revealed the remarkable potential of brain organoids for studying the migration of inhibitory neurons.
