The lentiviral expression plasmid pHRsinUbEm was a gift from Paul J. Lehner (University of Cambridge). TNFR1 expression plasmids were co-transfected with the vesicular stomatitis virus-G envelope plasmid pMD2.G and packaging plasmid psPAX2 (containing HIV-1 Gag and Rev) into HEK 293T cells to package lentiviral particles. Viral titers were determined by serial dilution and transduction of HEK 293T cells.
