the surrounding tissue and are often either attached directly to the kidney or within the connective tissue just anterior to the organ. The adrenal glands of males are generally smaller than those of females. The sample was transferred to the stage of a Zeiss dissecting microscope to facilitate clean dissection of the adrenal gland from the surrounding tissue. Once the adrenal glands had been separated they were weighed on a Mettler Toledo scale to a 10th of a milligram, fixed and stored for subsequent histological analysis.