DSU Spinning Disk Confocal on an IX81 inverted microscope. Cells were counted using Stereo Investigator image capture equipment and software. For cell counting on cover slips, an optical fractionator probe was used with a 500 µm × 500 µm grid size and 100 µm × 100 mm counting frame (>40 counting sites with >1000 total cells counted per sample) at 40 × magnification. Cover slips from 3–4 independent differentiations were used for analysis. For statistical analysis, we performed a t-test (alpha = 0.05) for comparison of control vs. sample using GraphPad Prism 6 software.
