The hippocampus is an interesting region in which to study nACh receptor β4 expression, as it is known that β4 expression in this structure significantly varies across mouse strains (Gahring et al., 2004). However, it is clear that across strains, there is β4 expression, both mRNA and protein, in the dentate gyrus, CA1 field, CA3 field and the subiculum (Dineley-Miller and Patrick, 1992; Gahring et al., 2004; Winzer-Serhan and Leslie, 1997). Consistent with these observations, we see strong β4 promoter activity in these same regions (Fig. 6A and Table 3).
