inoculated animals only exhibited evidence of gross infection on one side (presumably the site of inoculation), and only that lung that was collected for plating. The unaffected lung did not show bacteria when plated in preliminary experiments and therefore was not routinely collected. The lung was homogenized using a tissue homogenizer. Serial dilutions of the lung homogenate were performed and then plated on MacConkey agar to determine CFUs of Klebsiella pneumoniae per lung. Plates were incubated overnight at 37°C and CFUs were counted manually.
