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Chunk #17 — MATERIALS AND METHODS — Statistical Analysis

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Postnatal binge-like alcohol exposure decreases dendritic complexity while increasing the density of mature spines in mPFC Layer II/III pyramidal neurons.
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Repeated measures ANOVA was used to compare Sholl analyses between groups, looking in particular at ring × treatment. One-way ANOVA followed by Turkey post hoc test were used to evaluate the effect of postnatal condition on both the basilar dendrites’ spine density in mPFC and the percentage of mature or immature spine phenotypes. One-way ANOVA was also used to evaluate group differences for total dendritic length. The SPSS statistical package was used for all analyses. The level of significance was set at p < 0.05 for all tests.