Induced neurons were removed from the 6 well plate surface using Accutase and plated in a 10 μl bolus of 20 000 iNs per compartment. Prior to adding the 10 μl bolus, all DMEM for glial cell seeding was removed via a pipette. Cells were allowed to attach to the surface for five hours, after which approximately 200 μl of Neurobasal medium with 5% FBS, 1% P/S, 10 ng ml−1 BDNF, 10 ng ml−1 GDNF, 10 ng ml−1 NT3, and 2 μg ml−1 doxycycline was added; care must be taken to avoid cellular crossing over the microchannel wall during seeding. After plating the iNs, 96 well plate cultures began a slow transition from Neurobasal medium to BrainPhys™ medium (Stem Cell Technologies). On day 4 after iN seeding, 50 μl were removed from each well and replaced with 50 μl of BrainPhys™ with L-glutamine and NeuroCult™ SM1 supplements with 1% P/S, 10 ng ml−1 BDNF, 10 ng ml−1 GDNF, 10 ng ml−1 NT3, and 2 μg ml−1 doxycycline. At day 8, 80 μl of media were removed from each well, followed
