Four SCZD patients were selected: patient 1, diagnosed at six years of age, had childhood-onset SCZD; patients 2, 3 and 4 were from families in which all offspring and one parent were affected with psychiatric disease (SI Fig. 3A). Primary human fibroblasts (HFs) were reprogrammed using inducible lentiviruses7. Control and SCZD hiPSCs expressed endogenous pluripotency genes, repressed viral genes and were indistinguishable in assays for self-renewal and pluripotency (Fig. 1; SI Fig. 2). SCZD hiPSCs had no apparent defects in generating neural progenitor cells (NPCs) or neurons (Fig. 1; SI Fig. 3). Most hiPSC neurons were presumably glutamatergic and expressed VGLUT1 (SI Fig. 8A). Approximately 30% of neurons were GAD67-positive (GABAergic) (SI Fig. 8C,D) whereas less than 10% of neurons were tyrosine hydroxylase (TH)-positive (dopaminergic) (SI Fig. 7).
