Recently there has been a growing interest in understanding the transcriptomic changes attributed to AD [18–25], as these may point to underlying molecular mechanisms of disease. These studies are typically designed to analyze the expression profiles of large cohorts ascertained from homogenized regions of the brain (e.g. bulk RNA-sequencing [RNA-seq]) of affected and control donors. However, as bulk RNA-seq captures the gene expression of all the constituent cells in the sampled tissue; the altered cellular composition associated with AD has been reported to confound downstream analyses [20].
