Gene expression estimates were determined using RNA samples from LCLs from 95 Hutterite individuals. The cells were cultured at 37°C and 5% CO2 in RPMI medium 1640 (containing L-glutamine) supplemented with 50 µg/ml gentamicin and 20% fetal bovine serum. Total RNA was extracted from each cell line using the RNeasy Mini Kit (Qiagen, Germantown, MD, USA). The quality and concentration of the RNA (Supplementary data, Table S8) were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Total RNA samples were sent to the Southern California Genotyping Consortium (SCGC) where they were processed and hybridized to Illumina HT-12 v3 Expression BeadChips (Illumina, San Diego, CA, USA). Samples were sent in two separate batches, which were balanced with respect to lymphocyte count, sex, age and relatedness.
