The biospecimen collection project of the GTEx pilot faced many challenges. Collecting biospecimens for research purposes using a novel preservation method (PAXgene Tissue) presented a new challenge for OPOs due to the fact that the staff needed to be trained in proper use of the preservative including the required step of switching from fix to stabilizer solutions. Ongoing feedback from project partners was essential to synergize project needs and local operations. Throughout the initiation, start-up and steady state phases of the pilot GTEx project, collaboration with all partners was paramount to the success of GTEx analysis, and continues to be an essential factor in the project.
