We tested the effect of osmolarity on the dose effect for ethanol on crawling, and found that the effect was significant, but more subtle than for swimming. At 10 minutes of exposure to 100 mM ethanol, the behavior of worms tested on NGM was less affected compared with worms tested on plates made with Dent's Saline (Figure 6a). We asked if the effect of osmolarity on the dose response for ethanol was due to altering the acute accumulation of ethanol. At 10 minutes of 100 mM ethanol exposure, worms on NGM plates accumulated significantly less ethanol than did animals on DS plates (Figure 6b).
