Chunk #11 — Materials and methods — Immunofluorescence microscopy, co-localization, and proximity ligation studies

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Pathological tau drives ectopic nuclear speckle scaffold protein SRRM2 accumulation in neuron cytoplasm in Alzheimer's disease.

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Proximity Ligation assays were performed using Duolink PLA technology as recommended by the manufacturer (Sigma-Aldrich Catalog# DUO92101,St. Louis, MO) using sections prepared through primary antibody incubation as described above. Human brain samples assayed for pSRRM2 and pS422 colocalization were imaged on a Nikon A1R confocal microscope using a 100 × oil immersion objective (Nikon USA, Melville, NY).