To identify differentially expressed genes across SPN populations, we first generated subcluster-level profiles by summing the integer counts of each cell in each SPN subcluster. Differentially expressed genes exhibited >2 fold differences and had P<10−100 based on the binomTest (Robinson et al., 2010) and were visualized in SPN scatterplots with large, dark dots. Non-eSPN subclusters (13–6, 13–7, 13–8, 13–9 and 13–10) were excluded from the differential expression analysis described in Figure S7I.
