The experimental subjects were 54 experimentally naive male Wistar rats weighing 250–356 g. At least 2 weeks prior to the experimental procedures rats were surgically prepared with recording electrodes in hippocampus (AP –3.0, ML ±3.0, DV –3.0), and amygdala (AP –1.0, ML ±5.3, DV –8.5). Stainless steel screw electrodes were also placed over frontal cortex and in the bony calvarium 3 mm posterior to lambda. Rats were administered ethanol (1.5 G/Kg and saline intraperitoneally (I.P.)) on separate days and the order of the administration was randomized. Ethanol was given in a 20% solution in saline. Electrophysiological recording were collected sixty minutes following drug/saline administration (Ehlers et al., 1998c). All experimental protocols were approved by the Institutional Animal Care and Use Committee at the Scripps Research Institute and were consistent with the guidelines of the NIH Guide for the Care and Use of Laboratory Animals (NIH Publication No. 80-23, revised 1996).
