Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
paper
Cited
Public
Unavailable
- Authors
- Holland, P M; Abramson, R D; Watson, R; Gelfand, D H
- Year
- 1991
- Journal
- Proceedings of the National Academy of Sciences of the United States of America
- PMID
- 1871133
- DOI
- 10.1073/pnas.88.16.7276
- PMCID
- PMC52277
The 5'----3' exonuclease activity of the thermostable enzyme Thermus aquaticus DNA polymerase may be employed in a polymerase chain reaction product detection system to generate a specific detectable signal concomitantly with amplification. An oligonucleotide probe, nonextendable at the 3' end, labeled at the 5' end, and designed to hybridize within the target sequence, is introduced into the polymerase chain reaction assay. Annealing of probe to one of the polymerase chain reaction product strands during the course of amplification generates a substrate suitable for exonuclease activity. During amplification, the 5'----3' exonuclease activity of T. aquaticus DNA polymerase degrades the probe into smaller fragments that can be differentiated from undegraded probe. The assay is sensitive and specific and is a significant improvement over more cumbersome detection methods.
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| A Basic Guide to Real Time PCR in Microbial Diagnostics: Definitions, Parameters, and Everything. | Kralik P et al. | โ | 2017 | โ |
| A Comprehensive Quality Evaluation System for Complex Herbal Medicine Using PacBio Sequencing, PCR-Denaturing Gradient Gel Electrophoresis, and Several Chemical Approaches. | Zheng X et al. | โ | 2017 | โ |
| A novel quantitative PCR mediated by high-fidelity DNA polymerase. | Zhang M et al. | โ | 2017 | โ |
| Association mapping of morphological traits in wild and captive zebra finches: reliable within, but not between populations. | Knief U et al. | โ | 2017 | โ |
| Calibration-free assays on standard real-time PCR devices. | Debski PR et al. | โ | 2017 | โ |
| Centrifugal micro-channel array droplet generation for highly parallel digital PCR. | Chen Z et al. | โ | 2017 | โ |
| Chromosome conformation and gene expression patterns differ profoundly in human fibroblasts grown in spheroids versus monolayers. | Chen H et al. | โ | 2017 | โ |
| Comparing expression and activity of PCSK9 in SPRET/EiJ and C57BL/6J mouse strains shows lack of correlation with plasma cholesterol. | Sirois F et al. | โ | 2017 | โ |
| Contribution of major FLM isoforms to temperature-dependent flowering in Arabidopsis thaliana. | Capovilla G et al. | โ | 2017 | โ |
| Droplet-Based Digital PCR: Application in Cancer Research. | Perkins G et al. | โ | 2017 | โ |
| Evaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay. | Nagy A et al. | โ | 2017 | โ |
| High-Throughput Genotyping with TaqMan Allelic Discrimination and Allele-Specific Genotyping Assays. | Heissl A et al. | โ | 2017 | โ |
| Is There a Role for Minimal Residual Disease Monitoring in Follicular Lymphoma in the Chemo-Immunotherapy Era? | Gritti G et al. | โ | 2017 | โ |
| Microgravity validation of a novel system for RNA isolation and multiplex quantitative real time PCR analysis of gene expression on the International Space Station. | Parra M et al. | โ | 2017 | โ |
| Multiplex Detection of Toxigenic Penicillium Species. | Rodrรญguez A et al. | โ | 2017 | โ |
| PCR-Based Detection Methods for Single-Nucleotide Polymorphism or Mutation: Real-Time PCR and Its Substantial Contribution Toward Technological Refinement. | Matsuda K | โ | 2017 | โ |
| PCR Technologies for Point of Care Testing: Progress and Perspectives. | Petralia S et al. | โ | 2017 | โ |
| Real-Time Methylation-Specific Polymerase Chain Reaction for MGMT Promoter Methylation Clinical Testing in Glioblastoma: An Alternative Detection Method for a Heterogeneous Process. | Ida CM et al. | โ | 2017 | โ |
| Sequence specific sorting of DNA molecules with FACS using 3dPCR. | Sukovich DJ et al. | โ | 2017 | โ |
| Serological and molecular prevalence of canine vector-borne diseases (CVBDs) in Korea. | Suh GH et al. | โ | 2017 | โ |
| Simultaneous detection of respiratory syncytial virus and human metapneumovirus by one-step multiplex real-time RT-PCR in patients with respiratory symptoms. | You HL et al. | โ | 2017 | โ |
| The cobasยฎ 6800/8800 System: a new era of automation in molecular diagnostics. | Cobb B et al. | โ | 2017 | โ |
| The influence of Osmunda regalis root extract on head and neck cancer cell proliferation, invasion and gene expression. | Schmidt M et al. | โ | 2017 | โ |
| A Multi-Species TaqMan PCR Assay for the Identification of Asian Gypsy Moths (Lymantria spp.) and Other Invasive Lymantriines of Biosecurity Concern to North America. | Stewart D et al. | โ | 2016 | โ |
| Analytical workflow of double-digest restriction site-associated DNA sequencing based on empirical and in silico optimization in tomato. | Shirasawa K et al. | โ | 2016 | โ |
| An efficient method for measuring copy number variation applied to improvement of nematode resistance in soybean. | Lee TG et al. | โ | 2016 | โ |
| A rapid and cost-effective method for genotyping apolipoprotein E gene polymorphism. | Zhong L et al. | โ | 2016 | โ |
| A sandwich-like strategy for the label-free detection of oligonucleotides by surface plasmon fluorescence spectroscopy (SPFS). | Su Q et al. | โ | 2016 | โ |
| Carboxylic Carbon Quantum Dots as a Fluorescent Sensing Platform for DNA Detection. | Loo AH et al. | โ | 2016 | โ |
| Cobalt oxyhydroxide nanoflake based fluorescence sensing platform for label-free detection of DNA. | Chang Y et al. | โ | 2016 | โ |
| Coming of age: ten years of next-generation sequencing technologies. | Goodwin S et al. | โ | 2016 | โ |
| Comparing qPCR and Nested PCR Diagnostic Methods for Aster Yellows Phytoplasma in Aster Leafhoppers. | Demeuse KL et al. | โ | 2016 | โ |
| Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns. | Tost J | โ | 2016 | โ |
| Developmental validation of the Quantifiler(ยฎ) HP and Trio Kits for human DNA quantification in forensic samples. | Holt A et al. | โ | 2016 | โ |
| Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays. | Kimura Y et al. | โ | 2016 | โ |
| Fluorogenic bidirectional displacement probe-based real-time isothermal DNA amplification and specific visual detection of products. | Ding X et al. | โ | 2016 | โ |
| Four-locus phylogeny of Fusarium avenaceum and related species and their species-specific identification based on partial phosphate permease gene sequences. | Stakheev AA et al. | โ | 2016 | โ |
| Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuant(ยฎ) system. | Ewing MM et al. | โ | 2016 | โ |
| Influence of DNA isolation method on the investigation of archaeal diversity and abundance in biogas plants. | Theiss J et al. | โ | 2016 | โ |
| Investigating the effect of autoinduction in cynomolgus monkeys of a novel anticancer MDM2 antagonist, idasanutlin, and relevance to humans. | Glenn KJ et al. | โ | 2016 | โ |
| MeltMan: Optimization, Evaluation, and Universal Application of a qPCR System Integrating the TaqMan qPCR and Melting Analysis into a Single Assay. | Nagy A et al. | โ | 2016 | โ |
| Mismatch discrimination of lipidated DNA and LNA-probes (LiNAs) in hybridization-controlled liposome assembly. | Jakobsen U et al. | โ | 2016 | โ |
| Monochrome Multiplexing in Polymerase Chain Reaction by Photobleaching of Fluorogenic Hydrolysis Probes. | Schuler F et al. | โ | 2016 | โ |
| Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review. | Irshad M et al. | โ | 2016 | โ |
| On-chip quantitative PCR using integrated real-time detection by capillary electrophoresis. | Liu Y et al. | โ | 2016 | โ |
| Quantitative Detection and Resolution of BRAF V600 Status in Colorectal Cancer Using Droplet Digital PCR and a Novel Wild-Type Negative Assay. | Bidshahri R et al. | โ | 2016 | โ |
| Rapid and reliable genotyping of HLA-B*58:01 in four Chinese populations using a single-tube duplex real-time PCR assay. | Kang X et al. | โ | 2016 | โ |
| Real-Time PCR-Based Quantitation Method for the Genetically Modified Soybean Line GTS 40-3-2. | Kitta K et al. | โ | 2016 | โ |
| Real-time PCR probe optimization using design of experiments approach. | Wadle S et al. | โ | 2016 | โ |
| Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA. | Kretschy N et al. | โ | 2016 | โ |
| Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR. | Tatti E et al. | โ | 2016 | โ |
| The Use of Real-Time Reverse Transcription-PCR for Assessing Estrogen Receptor and Estrogen-Responsive Gene Expression. | Booze ML et al. | โ | 2016 | โ |
| Trichomonas vaginalis: An Updated Overview Towards Diagnostic Improvement. | Oliveira AS et al. | โ | 2016 | โ |
| Ultrasensitive FRET-based DNA sensor using PNA/DNA hybridization. | Yang LH et al. | โ | 2016 | โ |
| An integrated closed-tube 2-plex PCR amplification and hybridization assay with switchable lanthanide luminescence based spatial detection. | Lahdenperรค S et al. | โ | 2015 | โ |
| A simple PCR-based fluorometric system for detection of mutant fusion DNAs using a quencher-free fluorescent DNA probe and graphene oxide. | Roh K et al. | โ | 2015 | โ |
| Calcium silicate/calcium phosphate biphasic cements for vital pulp therapy: chemical-physical properties and human pulp cells response. | Gandolfi MG et al. | โ | 2015 | โ |
| Clinical Applications of Quantitative Real-Time PCR in Virology. | Engstrom-Melnyk J et al. | โ | 2015 | โ |
| Cost-effective optimization of real-time PCR-based detection of Campylobacter and Salmonella with inhibitor tolerant DNA polymerases. | Fachmann MS et al. | โ | 2015 | โ |
| Design of primers and probes for quantitative real-time PCR methods. | Rodrรญguez A et al. | โ | 2015 | โ |
| Design, synthesis, and application of Spinach molecular beacons triggered by strand displacement. | Bhadra S et al. | โ | 2015 | โ |
| Diagnosis and Management of Hepatitis C Virus Infection. | Mukherjee R et al. | โ | 2015 | โ |
| Diagnosis of Phytoplasmas by Real-Time PCR Using Locked Nucleic Acid (LNA) Probes. | Palmano S et al. | โ | 2015 | โ |
| [Digital PCR compartmentalization I. Single-molecule detection of rare mutations]. | Perez-Toralla K et al. | โ | 2015 | โ |
| Doubling Throughput of a Real-Time PCR. | Ahrberg CD et al. | โ | 2015 | โ |
| Early detection of dengue virus by use of reverse transcription-recombinase polymerase amplification. | Teoh BT et al. | โ | 2015 | โ |
| Ecology and biotechnological potential of the thermophilic fermentative Coprothermobacter spp. | Gagliano MC et al. | โ | 2015 | โ |
| Erythroid differentiation ability of butyric acid analogues: identification of basal chemical structures of new inducers of foetal haemoglobin. | Bianchi N et al. | โ | 2015 | โ |
| Evaluation of infectivity and reverse transcriptase real-time polymerase chain reaction assays for detection of xenotropic murine leukemia virus used in virus clearance validation. | Anwaruzzaman M et al. | โ | 2015 | โ |
| Exponential quadruplex priming amplification for DNA-based isothermal diagnostics. | Partskhaladze T et al. | โ | 2015 | โ |
| Gene analysis of signal-joint T cell receptor excision circles and their relationship to age in dogs. | Ito G et al. | โ | 2015 | โ |
| HLA Genotyping: Methods for the Identification of the HLA-DQ2,-DQ8 Heterodimers Implicated in Celiac Disease (CD) Susceptibility. | Fasano ME et al. | โ | 2015 | โ |
| Imputation of KIR Types from SNP Variation Data. | Vukcevic D et al. | โ | 2015 | โ |
| Mitochondrial genome sequencing and development of genetic markers for the detection of DNA of invasive bighead and silver carp (Hypophthalmichthys nobilis and H. molitrix) in environmental water samples from the United States. | Farrington HL et al. | โ | 2015 | โ |
| Molecular approaches to recognize relevant and emerging infectious diseases in animals. | Granberg F et al. | โ | 2015 | โ |
| Molecular characterisation of ABC-type multidrug efflux systems in Bifidobacterium longum. | Moodley C et al. | โ | 2015 | โ |
| Molecular methods for microbiological quality control of meat and meat products: a review. | Gokulakrishnan P et al. | โ | 2015 | โ |
| PCR-activated cell sorting for cultivation-free enrichment and sequencing of rare microbes. | Lim SW et al. | โ | 2015 | โ |
| Quantitative analysis of classical and new putative periodontal pathogens in subgingival biofilm: a case-control study. | Al-hebshi NN et al. | โ | 2015 | โ |
| Quantitative high resolution melting: two methods to determine SNP allele frequencies from pooled samples. | Capper RL et al. | โ | 2015 | โ |
| Rapid and quantitative detection of hepatitis B virus. | Liu YP et al. | โ | 2015 | โ |
| Real-time PCR detection chemistry. | Navarro E et al. | โ | 2015 | โ |
| Real-time PCR protocols for the quantification of the begomovirus tomato yellow leaf curl Sardinia virus in tomato plants and in its insect vector. | Noris E et al. | โ | 2015 | โ |
| Relevance of retrovirus quantification in cerebrospinal fluid for neurologic diagnosis. | Rosadas C et al. | โ | 2015 | โ |
| Simulation-guided DNA probe design for consistently ultraspecific hybridization. | Wang JS et al. | โ | 2015 | โ |
| Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities. | Hodne K et al. | โ | 2015 | โ |
| Small-molecule auxin inhibitors that target YUCCA are powerful tools for studying auxin function. | Kakei Y et al. | โ | 2015 | โ |
| Surface invasive cleavage assay on a maskless light-directed diamond DNA microarray for genome-wide human SNP mapping. | Nie B et al. | โ | 2015 | โ |
| Temporal dynamics of inflammatory cytokines/chemokines during sofosbuvir and ribavirin therapy for genotype 2 and 3 hepatitis C infection. | Carlin AF et al. | โ | 2015 | โ |
| Universal conventional and real-time PCR diagnosis tools for Sarcoptes scabiei. | Angelone-Alasaad S et al. | โ | 2015 | โ |
| Using Next Generation Sequencing for Multiplexed Trait-Linked Markers in Wheat. | Bernardo A et al. | โ | 2015 | โ |
| Visualization of nucleic acids with synthetic exciton-controlled fluorescent oligonucleotide probes. | Wang DO et al. | โ | 2015 | โ |
| A droplet digital PCR detection method for rare L1 insertions in tumors. | White TB et al. | โ | 2014 | โ |
| A miniature quantitative PCR device for directly monitoring a sample processing on a microfluidic rapid DNA system. | Hurth C et al. | โ | 2014 | โ |
| An investigation of PCR inhibition using Plexor(ยฎ) -based quantitative PCR and short tandem repeat amplification. | Thompson RE et al. | โ | 2014 | โ |
| A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus. | Yang L et al. | โ | 2014 | โ |
| Application of the ASLP technology to a novel platform for rapid and noise-free multiplexed SNP genotyping. | Shin SC et al. | โ | 2014 | โ |
| A "turn on/off" scorpion biosensor targeting point mutation of SMN genes for diagnosis of spinal muscular atrophy. | Chen CA et al. | โ | 2014 | โ |
| Avian influenza virus detection and quantitation by real-time RT-PCR. | Spackman E | โ | 2014 | โ |
| Bi-allelic SNP genotyping using the TaqManยฎ assay. | Woodward J | โ | 2014 | โ |
| Circulating microRNAs: understanding the limits for quantitative measurement by real-time PCR. | Hardikar AA et al. | โ | 2014 | โ |
| CLE6 expression recovers gibberellin deficiency to promote shoot growth in Arabidopsis. | Bidadi H et al. | โ | 2014 | โ |
| Closed-tube human leukocyte antigen DQA1โ05 genotyping assay based on switchable lanthanide luminescence probes. | Lehmusvuori A et al. | โ | 2014 | โ |
| Comparison of RT-PCR assay and virus isolation in cell culture for the detection of Alkhumra hemorrhagic fever virus. | Madani TA et al. | โ | 2014 | โ |
| Compartmentalized self-replication under fast PCR cycling conditions yields Taq DNA polymerase mutants with increased DNA-binding affinity and blood resistance. | Arezi B et al. | โ | 2014 | โ |
| Complete genome sequencing and genetic characterization of Alkhumra hemorrhagic fever virus isolated from Najran, Saudi Arabia. | Madani TA et al. | โ | 2014 | โ |
| Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR. | Takei F et al. | โ | 2014 | โ |
| Detection of deoxyribonucleic acid (DNA) targets using polymerase chain reaction (PCR) and paper surface-enhanced Raman spectroscopy (SERS) chromatography. | Hoppmann EP et al. | โ | 2014 | โ |
| DNA fingerprinting in botany: past, present, future. | Nybom H et al. | โ | 2014 | โ |
| Effective and site-specific phosphoramidation reaction for universally labeling nucleic acids. | Su YC et al. | โ | 2014 | โ |
| Effect of a high-fat--high-fructose diet, stress and cinnamon on central expression of genes related to immune system, hypothalamic-pituitary-adrenocortical axis function and cerebral plasticity in rats. | Marissal-Arvy N et al. | โ | 2014 | โ |
| Elevated cell-free plasma DNA level as an independent predictor of mortality in patients with severe traumatic brain injury. | Rodrigues Filho EM et al. | โ | 2014 | โ |
| Enzyme-assisted target recycling (EATR) for nucleic acid detection. | Gerasimova YV et al. | โ | 2014 | โ |
| Fluorescent-increase kinetics of different fluorescent reporters used for qPCR depend on monitoring chemistry, targeted sequence, type of DNA input and PCR efficiency. | Ruijter JM et al. | โ | 2014 | โ |
| From genes to markers: exploiting gene sequence information to develop tools for plant breeding. | Garcia M et al. | โ | 2014 | โ |
| Genome-wide association study of alcohol dependence:significant findings in African- and European-Americans including novel risk loci. | Gelernter J et al. | โ | 2014 | โ |
| Genome-wide association study of cocaine dependence and related traits: FAM53B identified as a risk gene. | Gelernter J et al. | โ | 2014 | โ |
| Genome-wide association study of opioid dependence: multiple associations mapped to calcium and potassium pathways. | Gelernter J et al. | โ | 2014 | โ |
| Genotyping single nucleotide polymorphisms using different molecular beacon multiplexed within a suspended core optical fiber. | Nguyen LV et al. | โ | 2014 | โ |
| Highly sensitive amplicon-based transcript quantification by semiconductor sequencing. | Zhang JD et al. | โ | 2014 | โ |
| iPS cell derived neuronal cells for drug discovery. | Heilker R et al. | โ | 2014 | โ |
| Molecular methods used for detection of minimal residual disease following hematopoietic stem cell transplantation in myeloid disorders. | Elmaagacli AH | โ | 2014 | โ |
| Orthogonal ion pairing reversed phase liquid chromatography purification of oligonucleotides with bulky fluorophores. | Anacleto C et al. | โ | 2014 | โ |
| Point of Care Technologies for HIV. | Haleyur Giri Setty MK et al. | โ | 2014 | โ |
| Propagation and titration of Alkhumra hemorrhagic fever virus in the brains of newborn Wistar rats. | Madani TA et al. | โ | 2014 | โ |
| Quantitative PCR for detection of mRNA and gDNA in environmental isolates. | Brzoska AJ et al. | โ | 2014 | โ |
| Root foraging elicits niche complementarity-dependent yield advantage in the ancient 'three sisters' (maize/bean/squash) polyculture. | Zhang C et al. | โ | 2014 | โ |
| Silver and magnetic nanoparticles for sensitive DNA detection by SERS. | Donnelly T et al. | โ | 2014 | โ |
| Single-channel multiplexing without melting curve analysis in real-time PCR. | Lee YJ et al. | โ | 2014 | โ |
| Subgingival periodontal pathogens associated with chronic periodontitis in Yemenis. | Al-Hebshi NN et al. | โ | 2014 | โ |
| A novel photoinduced electron transfer (PET) primer technique for rapid real-time PCR detection of Cryptosporidium spp. | Jothikumar N et al. | โ | 2013 | โ |
| A portable reverse transcription recombinase polymerase amplification assay for rapid detection of foot-and-mouth disease virus. | Abd El Wahed A et al. | โ | 2013 | โ |
| Application of quantitative PCR for the detection of microorganisms in water. | Botes M et al. | โ | 2013 | โ |
| Applications of physiological bases of ageing to forensic sciences. Estimation of age-at-death. | C Zapico S et al. | โ | 2013 | โ |
| Astaxanthin suppresses MPP(+)-induced oxidative damage in PC12 cells through a Sp1/NR1 signaling pathway. | Ye Q et al. | โ | 2013 | โ |
| Betaine significantly improves multiplex tetra-primer ARMS-PCR methods. | Lajin B et al. | โ | 2013 | โ |
| CdTe quantum dots enhance feasibility of EvaGreen-based real-time PCR with decent amplification fidelity. | Sang F et al. | โ | 2013 | โ |
| Clinical implications and prognostic role of minimal residual disease detection in follicular lymphoma. | Lobetti-Bodoni C et al. | โ | 2013 | โ |
| Comparative evaluation of conventional polymerase chain reaction (PCR), with loop-mediated isothermal amplification and SYBR green I-based real-time PCR for the quantitation of porcine circovirus-1 DNA in contaminated samples destined for vaccine production. | Yang BC et al. | โ | 2013 | โ |
| Current methods for fluorescence-based universal sequence-dependent detection of nucleic acids in homogenous assays and clinical applications. | Faltin B et al. | โ | 2013 | โ |
| Detecting polymorphisms in human longevity studies: HLA typing and SNP genotyping by amplicon sequencing. | Vargas-Alarcรณn G et al. | โ | 2013 | โ |
| Detection, enumeration, and characterization of immune cells infiltrating melanoma tumors. | Toh B et al. | โ | 2013 | โ |
| Detection of Circulating Tumour Cells from Blood of Breast Cancer Patients via RT-qPCR. | Andergassen U et al. | โ | 2013 | โ |
| Detection of circulating tumour cells on mRNA levels with established breast cancer cell lines. | Zebisch M et al. | โ | 2013 | โ |
| Detection of histone modification by chromatin immunoprecipitation combined zinc finger luciferase-based bioluminescence resonance energy transfer assay. | Yoshida W et al. | โ | 2013 | โ |
| Developmental regulation of diacylglycerol acyltransferase family gene expression in tung tree tissues. | Cao H et al. | โ | 2013 | โ |
| Development and characterization of the cobas human papillomavirus test. | Rao A et al. | โ | 2013 | โ |
| DNA/RNA Detection Using DNA-Templated Few-Atom Silver Nanoclusters. | Obliosca JM et al. | โ | 2013 | โ |
| DNAzyme based gap-LCR detection of single-nucleotide polymorphism. | Zhou L et al. | โ | 2013 | โ |
| Electrochemical real-time nucleic acid amplification: towards point-of-care quantification of pathogens. | Patterson AS et al. | โ | 2013 | โ |
| Evaluation of inhibitor-resistant real-time PCR methods for diagnostics in clinical and environmental samples. | Trombley Hall A et al. | โ | 2013 | โ |
| Evidence for cross-talk between the LH receptor and LH during implantation in mice. | Gridelet V et al. | โ | 2013 | โ |
| Gene expression alterations in bipolar disorder postmortem brains. | Chen H et al. | โ | 2013 | โ |
| Genetics and the history of the Samaritans: Y-chromosomal microsatellites and genetic affinity between Samaritans and Cohanim. | Oefner PJ et al. | โ | 2013 | โ |
| Genome-Wide Association Study Reveals Host Genetic Factors for Liver Diseases. | Nishida N et al. | โ | 2013 | โ |
| High throughput molecular confirmation of ฮฒ-thalassemia mutations using novel TaqMan probes. | Kho SL et al. | โ | 2013 | โ |
| Intrinsic cardiac origin of human cardiosphere-derived cells. | White AJ et al. | โ | 2013 | โ |
| Magneto-capillary valve for integrated purification and enrichment of nucleic acids and proteins. | den Dulk RC et al. | โ | 2013 | โ |
| Monocyte chemoattractant protein-1 affects migration of hippocampal neural progenitors following status epilepticus in rats. | Hung YW et al. | โ | 2013 | โ |
| Multiplex detection of DNA mutations by the fluorescence fingerprint spectrum technique. | Song J et al. | โ | 2013 | โ |
| Polymerase chain displacement reaction. | Harris CL et al. | โ | 2013 | โ |
| Portable nucleic acid thermocyclers. | Almassian DR et al. | โ | 2013 | โ |
| Quantifying the transcriptional output of single alleles in single living mammalian cells. | Yunger S et al. | โ | 2013 | โ |
| Quantitative PCR for glucose transporter and tristetraprolin family gene expression in cultured mouse adipocytes and macrophages. | Cao H et al. | โ | 2013 | โ |
| RNA pathogen detection with one-step reverse transcription PCR and strand-displacement based signal amplification. | Du F et al. | โ | 2013 | โ |
| Simultaneous DNA amplification and detection using a pH-sensing semiconductor system. | Toumazou C et al. | โ | 2013 | โ |
| Sofosbuvir for hepatitis C genotype 2 or 3 in patients without treatment options. | Jacobson IM et al. | โ | 2013 | โ |
| Sofosbuvir for previously untreated chronic hepatitis C infection. | Lawitz E et al. | โ | 2013 | โ |
| Stochastic loss of silencing of the imprinted Ndn/NDN allele, in a mouse model and humans with prader-willi syndrome, has functional consequences. | Rieusset A et al. | โ | 2013 | โ |
| Susceptibility of tick cell lines to infection with Alkhumra haemorrhagic fever virus. | Madani TA et al. | โ | 2013 | โ |
| The effect of lung cancer on cytokine expression in peripheral blood mononuclear cells. | Chang DH et al. | โ | 2013 | โ |
| The presence of two different target-site resistance mechanisms in individual plants of Alopecurus myosuroides Huds., identified using a quick molecular test for the characterisation of six ALS and seven ACCase SNPs. | Marshall R et al. | โ | 2013 | โ |
| Use of a large Stokes-shift fluorophore to increase the multiplexing capacity of a point-of-care DNA diagnostic device. | Richardson JA et al. | โ | 2013 | โ |
| Validation of a quantitative real-time PCR assay for HTLV-1 proviral load in peripheral blood mononuclear cells. | Rosadas C et al. | โ | 2013 | โ |
| Vitamin A regulates hypothalamic-pituitary-adrenal axis status in LOU/C rats. | Marissal-Arvy N et al. | โ | 2013 | โ |
| ABO blood group genotyping by quenching probe method. | Tsutsumi H et al. | โ | 2012 | โ |
| A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers. | Hall LM et al. | โ | 2012 | โ |
| A method for measuring gene copy number in biological samples without using control samples of known copies. | Ji W et al. | โ | 2012 | โ |
| Analysis of nonsense-mediated mRNA decay in mammalian cells. | Nicholson P et al. | โ | 2012 | โ |
| A novel rhodanese is required to maintain chloroplast translation in Chlamydomonas. | Luo L et al. | โ | 2012 | โ |
| A novel single fluorophore-labeled double-stranded oligonucleotide probe for fluorescence-enhanced nucleic acid detection based on the inherent quenching ability of deoxyguanosine bases and competitive strand-displacement reaction. | Zhang Y et al. | โ | 2012 | โ |
| A practicable detection system for genetically modified rice by SERS-barcoded nanosensors. | Chen K et al. | โ | 2012 | โ |
| Association between genetic variations in surfactant protein d and emphysema, interstitial pneumonia, and lung cancer in a Japanese population. | Ishii T et al. | โ | 2012 | โ |
| Astaxanthin protects against MPP(+)-induced oxidative stress in PC12 cells via the HO-1/NOX2 axis. | Ye Q et al. | โ | 2012 | โ |
| Automated high multiplex qPCR platform for simultaneous detection and quantification of multiple nucleic acid targets. | Hlousek L et al. | โ | 2012 | โ |
| CNR1 genotype influences HDL-cholesterol response to change in dietary fat intake. | Silver HJ et al. | โ | 2012 | โ |
| Comparison of real-time PCR and MassTag PCR for the multiplex detection of highly pathogenic agents. | Doellinger J et al. | โ | 2012 | โ |
| Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues. | Cao H et al. | โ | 2012 | โ |
| Detection and quantification of a radiation-associated mitochondrial DNA deletion by a nested real-time PCR in human peripheral lymphocytes. | Kim EJ et al. | โ | 2012 | โ |
| Detection of chicken and turkey meat in meat mixtures by using real-time PCR assays. | Kesmen Z et al. | โ | 2012 | โ |
| Detection of genetically modified DNA in fresh and processed foods sold in Kuwait. | Al-Salameen F et al. | โ | 2012 | โ |
| Development of TaqMan probe-based insulated isothermal PCR (iiPCR) for sensitive and specific on-site pathogen detection. | Tsai YL et al. | โ | 2012 | โ |
| Digital polymerase chain reaction in an array of femtoliter polydimethylsiloxane microreactors. | Men Y et al. | โ | 2012 | โ |
| Epidermal growth factor receptor mutation status in circulating free DNA in serum: from IPASS, a phase III study of gefitinib or carboplatin/paclitaxel in non-small cell lung cancer. | Goto K et al. | โ | 2012 | โ |
| Epigallocatechin-3-gallate suppresses 1-methyl-4-phenyl-pyridine-induced oxidative stress in PC12 cells via the SIRT1/PGC-1ฮฑ signaling pathway. | Ye Q et al. | โ | 2012 | โ |
| Fluorescent signatures for variable DNA sequences. | Rice JE et al. | โ | 2012 | โ |
| Gabra5-gene haplotype block associated with behavioral properties of the full agonist benzodiazepine chlordiazepoxide. | Clรฉment Y et al. | โ | 2012 | โ |
| Genotyping of single nucleotide polymorphisms by 5' nuclease allelic discrimination. | Malkki M et al. | โ | 2012 | โ |
| High-performance closed-tube PCR based on switchable luminescence probes. | Lehmusvuori A et al. | โ | 2012 | โ |
| Isothermal nucleic acid amplification technologies for point-of-care diagnostics: a critical review. | Craw P et al. | โ | 2012 | โ |
| Mediator probe PCR: a novel approach for detection of real-time PCR based on label-free primary probes and standardized secondary universal fluorogenic reporters. | Faltin B et al. | โ | 2012 | โ |
| Molecular diagnostic and predictive tests in the evolution of chronic hepatitis C anti-viral therapies. | Colucci G | โ | 2012 | โ |
| Multiplex detection and SNP genotyping in a single fluorescence channel. | Fu G et al. | โ | 2012 | โ |
| Mutation scanning using MUT-MAP, a high-throughput, microfluidic chip-based, multi-analyte panel. | Patel R et al. | โ | 2012 | โ |
| [NSI+] determinant has a pleiotropic phenotypic manifestation that is modulated by SUP35, SUP45, and VTS1 genes. | Nizhnikov AA et al. | โ | 2012 | โ |
| Nucleic acid probe containing fluorescent tricyclic base-linked acyclonucleoside for detection of single nucleotide polymorphisms. | Furukawa K et al. | โ | 2012 | โ |
| Pyrene-modified unlocked nucleic acids: synthesis, thermodynamic studies, and fluorescent properties. | Karlsen KK et al. | โ | 2012 | โ |
| Quantitative real-time PCR (qPCR) detection chemistries affect enumeration of the Dehalococcoides 16S rRNA gene in groundwater. | Hatt JK et al. | โ | 2012 | โ |
| Quantitative RT-PCR based platform for rapid quantification of the transcripts of highly homologous multigene families and their members during grain development. | Kaczmarczyk A et al. | โ | 2012 | โ |
| Quencher-free multiplexed monitoring of DNA reaction circuits. | Padirac A et al. | โ | 2012 | โ |
| Robust quantification of polymerase chain reactions using global fitting. | Carr AC et al. | โ | 2012 | โ |
| Selective quantification of human DNA by real-time PCR of FOXP2. | Soejima M et al. | โ | 2012 | โ |
| Sequence-based marker development in wheat: advances and applications to breeding. | Paux E et al. | โ | 2012 | โ |
| Simultaneous multiple target detection in real-time loop-mediated isothermal amplification. | Tanner NA et al. | โ | 2012 | โ |
| Specific detection of DNA through coupling of a TaqMan assay with surface enhanced Raman scattering (SERS). | Harper MM et al. | โ | 2012 | โ |
| Successful propagation of Alkhumra (misnamed as Alkhurma) virus in C6/36 mosquito cells. | Madani TA et al. | โ | 2012 | โ |
| Superiority of the buffy coat over serum or plasma for the detection of Alkhumra virus RNA using real time RT-PCR. | Madani TA et al. | โ | 2012 | โ |
| The effect of base-pair sequence on electrochemically driven denaturation. | Johnson RP et al. | โ | 2012 | โ |
| The relationship between body mass index, activated proteinย C resistance and risk of venous thrombosis. | Christiansen SC et al. | โ | 2012 | โ |
| The use of automated sequencing techniques to investigate the sequence selectivity of DNA-damaging agents. | Murray V et al. | โ | 2012 | โ |
| Transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by Ceratocystis fimbriata in leaves and storage roots. | Muramoto N et al. | โ | 2012 | โ |
| Two-dye and one- or two-quencher DNA probes for real-time PCR assay: synthesis and comparison with a TaqManโข probe. | Ryazantsev DY et al. | โ | 2012 | โ |
| Two new rapid SNP-typing methods for classifying Mycobacterium tuberculosis complex into the main phylogenetic lineages. | Stucki D et al. | โ | 2012 | โ |
| Ultra-selective and sensitive DNA detection by a universal apurinic/apyrimidinic probe-based endonuclease IV signal amplification system. | Xiao X et al. | โ | 2012 | โ |
| Use of an automated capillary DNA sequencer to investigate the interaction of cisplatin with telomeric DNA sequences. | Paul M et al. | โ | 2012 | โ |
| Visual detection of DNA mutation using multicolor fluorescent coding. | Song J et al. | โ | 2012 | โ |
| A latest and promising approach for prediction of viral load in hepatitis B virus infected patients. | Yalamanchili N et al. | โ | 2011 | โ |
| A model for transition of 5'-nuclease domain of DNA polymerase I from inert to active modes. | Xie P et al. | โ | 2011 | โ |
| A novel poisson distribution-based approach for testing boundaries of real-time PCR assays for food pathogen quantification. | Rossmanith P et al. | โ | 2011 | โ |
| A novel real-time PCR method based on signaling-by-incorporation. | Kim DR et al. | โ | 2011 | โ |
| Association between interleukin-6 receptor gene variations and atherosclerotic lipid profiles among young adolescents in Taiwan. | Chu NF et al. | โ | 2011 | โ |
| Association of serotonin transporter gene variation with smoking, chronic obstructive pulmonary disease, and its depressive symptoms. | Ishii T et al. | โ | 2011 | โ |
| Asynchronous PCR. | Chen C et al. | โ | 2011 | โ |
| A vertically stacked, polymer, microfluidic point mutation analyzer: rapid high accuracy detection of low-abundance K-ras mutations. | Han K et al. | โ | 2011 | โ |
| Colorimetric detection of PCR product with DNAzymes induced by 5'-nuclease activity of DNA polymerases. | Du F et al. | โ | 2011 | โ |
| Combined effect of C-reactive protein gene SNP +2147 A/G and interleukin-6 receptor gene SNP rs2229238 C/T on anthropometric characteristics among school children in Taiwan. | Lin FH et al. | โ | 2011 | โ |
| Conjugation polymer nanobelts: a novel fluorescent sensing platform for nucleic acid detection. | Wang L et al. | โ | 2011 | โ |
| Coordination polymer nanobelts for nucleic acid detection. | Luo Y et al. | โ | 2011 | โ |
| Detection of adeno- and lentiviral (HIV1) contaminations on laboratory surfaces as a tool for the surveillance of biosafety standards. | Bagutti C et al. | โ | 2011 | โ |
| Detection of KIAA1549-BRAF fusion transcripts in formalin-fixed paraffin-embedded pediatric low-grade gliomas. | Tian Y et al. | โ | 2011 | โ |
| Detection of MET and SOX2 amplification by quantitative real-time PCR in non-small cell lung carcinoma. | Cai YR et al. | โ | 2011 | โ |
| Detection of reverse transcription-PCR products by a simple and rapid light scattering technique. | Fan Y et al. | โ | 2011 | โ |
| DNA-mounted self-assembly: new approaches for genomic analysis and SNP detection. | Bichenkova EV et al. | โ | 2011 | โ |
| Fluorescence-enhanced potassium ions detection based on inherent quenching ability of deoxyguanosines and K(+)-induced conformational transition of G-rich ssDNA from duplex to G-quadruplex structures. | Zhang Y et al. | โ | 2011 | โ |
| Fluorogenic polymerase, endonuclease, and ligase assays based on DNA substrates labeled with a single fluorophore. | Nikiforov TT | โ | 2011 | โ |
| Genetic variation at CHRNA5-CHRNA3-CHRNB4 interacts with smoking status to influence body mass index. | Freathy RM et al. | โ | 2011 | โ |
| Homogeneous label-free genotyping of single nucleotide polymorphism using ligation-mediated strand displacement amplification with DNAzyme-based chemiluminescence detection. | Wang HQ et al. | โ | 2011 | โ |
| Increased levels of circulating DNA in patients with systemic autoimmune diseases: A possible marker of disease activity in Sjรถgren's syndrome. | Bartoloni E et al. | โ | 2011 | โ |
| Minimal residual disease detection in lymphoma and multiple myeloma: impact on therapeutic paradigms. | Ferrero S et al. | โ | 2011 | โ |
| Molecular beacons: powerful tools for imaging RNA in living cells. | Monroy-Contreras R et al. | โ | 2011 | โ |
| Monitoring the site-specific incorporation of dual fluorophore-quencher base analogues for target DNA detection by an unnatural base pair system. | Yamashige R et al. | โ | 2011 | โ |
| Nano-C(60) : a novel, effective, fluorescent sensing platform for biomolecular detection. | Li H et al. | โ | 2011 | โ |
| Nucleic acid-based fluorescent probes and their analytical potential. | Juskowiak B | โ | 2011 | โ |
| One-step homogeneous DNA assay with single-nanoparticle detection. | Han G et al. | โ | 2011 | โ |
| Poly(o-phenylenediamine) colloid-quenched fluorescent oligonucleotide as a probe for fluorescence-enhanced nucleic acid detection. | Tian J et al. | โ | 2011 | โ |
| Profiling microRNAs by real-time PCR. | Jacobsen N et al. | โ | 2011 | โ |
| Quantitative analysis of TEM-8 and CEA tumor markers indicating free tumor cells in the peripheral blood of colorectal cancer patients. | Raeisossadati R et al. | โ | 2011 | โ |
| Rapid discrimination of Tomato chlorosis virus, Tomato infectious chlorosis virus and co-amplification of plant internal control using real-time RT-PCR. | Papayiannis LC et al. | โ | 2011 | โ |
| Real-time electrochemical PCR with a DNA intercalating redox probe. | Defรฉver T et al. | โ | 2011 | โ |
| Recent advances and applications in QDs-based sensors. | Ma Q et al. | โ | 2011 | โ |
| RNase H-dependent PCR (rhPCR): improved specificity and single nucleotide polymorphism detection using blocked cleavable primers. | Dobosy JR et al. | โ | 2011 | โ |
| Self-dissociative primers for nucleic acid amplification and detection based on DNA quadruplexes with intrinsic fluorescence. | Kankia BI | โ | 2011 | โ |
| Substance P (SP)-neurokinin-1 receptor (NK-1R) alters adipose tissue responses to high-fat diet and insulin action. | Karagiannides I et al. | โ | 2011 | โ |
| The COBAS ยฎ TaqMan ยฎ hepatitis C virus assays: automated systems for accurate and sensitive viral load quantification. | Colucci G et al. | โ | 2011 | โ |
| Transcriptome sequencing and high-resolution melt analysis advance single nucleotide polymorphism discovery in duplicated salmonids. | Seeb JE et al. | โ | 2011 | โ |
| Use of duplex mutation primers for real-time PCR quantification of hepatitis C virus RNA in serum. | Xia QF et al. | โ | 2011 | โ |
| Use of qPCR for the study of hepatotoxic cyanobacteria population dynamics. | Martins A et al. | โ | 2011 | โ |
| ฮ-PCR, A Simple Method to Detect Translocations and Insertion/Deletion Mutations. | Lin MT et al. | โ | 2011 | โ |
| A common CNR1 (cannabinoid receptor 1) haplotype attenuates the decrease in HDL cholesterol that typically accompanies weight gain. | Feng Q et al. | โ | 2010 | โ |
| A dried tofu-supplemented diet affects mRNA expression of inflammatory cytokines in human blood. | Tovar V et al. | โ | 2010 | โ |
| A graphene-enhanced molecular beacon for homogeneous DNA detection. | Li F et al. | โ | 2010 | โ |
| A molecular inversion probe assay for detecting alternative splicing. | Lin S et al. | โ | 2010 | โ |
| Analysis of wide-domain transcriptional regulation in solid-state cultures of Aspergillus oryzae. | McKelvey SM et al. | โ | 2010 | โ |
| A novel in vitro model to investigate behavior of articular chondrocytes in osteoarthritis. | Rankin KS et al. | โ | 2010 | โ |
| Application of single nucleotide polymorphisms to non-model species: a technical review. | Garvin MR et al. | โ | 2010 | โ |
| A theoretical introduction to "combinatory SYBRGreen qPCR screening", a matrix-based approach for the detection of materials derived from genetically modified plants. | Van den Bulcke M et al. | โ | 2010 | โ |
| Cervical cytology biobanking in Europe. | Arbyn M et al. | โ | 2010 | โ |
| Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection. | Buh Gasparic M et al. | โ | 2010 | โ |
| Comparison of three methods for genotyping of prothrombotic polymorphisms. | Bianchi M et al. | โ | 2010 | โ |
| Correction of underquantification of human immunodeficiency virus type 1 load with the second version of the Roche Cobas AmpliPrep/Cobas TaqMan assay. | De Bel A et al. | โ | 2010 | โ |
| Current perspectives on detection of microbial contamination in bioethanol fermentors. | Muthaiyan A et al. | โ | 2010 | โ |
| Designed thiazole orange nucleotides for the synthesis of single labelled oligonucleotides that fluoresce upon matched hybridization. | Bethge L et al. | โ | 2010 | โ |
| Development of novel SNP system for individual and pedigree control in a Japanese Black cattle population using whole-genome genotyping assay. | Hara K et al. | โ | 2010 | โ |
| Differentiation of Tomato yellow leaf curl virus and Tomato yellow leaf curl Sardinia virus using real-time TaqMan PCR. | Papayiannis LC et al. | โ | 2010 | โ |
| Direct detection of mutant DNA in a mixed population of higher copy number wild-type DNA based on ligase detection reaction in conjunction with fluorescence resonance energy transfer. | Hashimoto M et al. | โ | 2010 | โ |
| Dried reagents for multiplex genotyping by tag-array minisequencing to be used in microfluidic devices. | Ahlford A et al. | โ | 2010 | โ |
| Effect of khat chewing on periodontal pathogens in subgingival biofilm from chronic periodontitis patients. | Al-Hebshi NN et al. | โ | 2010 | โ |
| Engineered DNA polymerases in biotechnology. | Kranaster R et al. | โ | 2010 | โ |
| Evaluation of the LightCycler 1536 Instrument for high-throughput quantitative real-time PCR. | Schlesinger J et al. | โ | 2010 | โ |
| Genetic susceptibility to different clinical forms of tuberculosis in the Peruvian population. | Taype CA et al. | โ | 2010 | โ |
| Geographical variation in resistance to acetyl-coenzyme A carboxylase-inhibiting herbicides across the range of the arable weed Alopecurus myosuroides (black-grass). | Dรฉlye C et al. | โ | 2010 | โ |
| High-throughput red blood cell antigen genotyping using a nanofluidic real-time polymerase chain reaction platform. | Hopp K et al. | โ | 2010 | โ |
| Homogeneous competitive hybridization assay based on two-photon excitation fluorescence resonance energy transfer. | Liu L et al. | โ | 2010 | โ |
| Massively parallel detection of gene expression in single cells using subnanolitre wells. | Gong Y et al. | โ | 2010 | โ |
| MicroRNA profiling of clear cell renal cell carcinoma by whole-genome small RNA deep sequencing of paired frozen and formalin-fixed, paraffin-embedded tissue specimens. | Weng L et al. | โ | 2010 | โ |
| Modulating molecular level space proximity: a simple and efficient strategy to design structured DNA probes. | Zheng J et al. | โ | 2010 | โ |
| New technologies in the genetic approach to sudden cardiac death in the young. | Brion M et al. | โ | 2010 | โ |
| Novel application of Locked Nucleic Acid chemistry for a Taqman assay for measuring diverse human immunodeficiency virus type 1 subtypes. | Li P et al. | โ | 2010 | โ |
| One-step RNA pathogen detection with reverse transcriptase activity of a mutated thermostable Thermus aquaticus DNA polymerase. | Kranaster R et al. | โ | 2010 | โ |
| PCSK9-deficient mice exhibit impaired glucose tolerance and pancreatic islet abnormalities. | Mbikay M et al. | โ | 2010 | โ |
| Pentaplexed quantitative real-time PCR assay for the simultaneous detection and quantification of botulinum neurotoxin-producing clostridia in food and clinical samples. | Kirchner S et al. | โ | 2010 | โ |
| PrimerBank: a resource of human and mouse PCR primer pairs for gene expression detection and quantification. | Spandidos A et al. | โ | 2010 | โ |
| Quality control for quantitative PCR based on amplification compatibility test. | Tichopad A et al. | โ | 2010 | โ |
| Quantitation of CD39 gene expression in pancreatic tissue by real-time polymerase chain reaction. | Loos M et al. | โ | 2010 | โ |
| Signal to cut-off (S/CO) ratio and detection of HCV genotype 1 by real-time PCR one-step method: is there any direct relationship? | Albertoni G et al. | โ | 2010 | โ |
| Simultaneous detection of bluetongue virus RNA, internal control GAPDH mRNA, and external control synthetic RNA by multiplex real-time PCR. | Vandenbussche F et al. | โ | 2010 | โ |
| Specific real-time reverse transcription-polymerase chain reaction for detection and quantitation of turkey coronavirus RNA in tissues and feces from turkeys infected with turkey coronavirus. | Chen YN et al. | โ | 2010 | โ |
| The ongoing evolution of qPCR. | Pfaffl MW | โ | 2010 | โ |
| Zip nucleic acids are potent hydrolysis probes for quantitative PCR. | Paris C et al. | โ | 2010 | โ |
| Accurate strand-specific quantification of viral RNA. | Plaskon NE et al. | โ | 2009 | โ |
| A cross comparison of QPCR to agar-based or defined substrate test methods for the determination of Escherichia coli and enterococci in municipal water quality monitoring programs. | Lavender JS et al. | โ | 2009 | โ |
| Advantages and limitations of quantitative PCR (Q-PCR)-based approaches in microbial ecology. | Smith CJ et al. | โ | 2009 | โ |
| A single nucleotide polymorphism melt curve assay employing an intercalating dye probe fluorescence resonance energy transfer for forensic analysis. | Halpern MD et al. | โ | 2009 | โ |
| A TaqMan-based real-time polymerase chain reaction for the detection of porcine parvovirus. | Chen HY et al. | โ | 2009 | โ |
| Characterisation of 5-HT3C, 5-HT3D and 5-HT3E receptor subunits: evolution, distribution and function. | Holbrook JD et al. | โ | 2009 | โ |
| Characterization of the 5' to 3' nuclease activity of Thermus aquaticus DNA polymerase on fluorogenic double-stranded probes. | Huang Q et al. | โ | 2009 | โ |
| Characterization of the porcine TOR1A gene: The first step towards generation of a pig model for dystonia. | Henriksen C et al. | โ | 2009 | โ |
| Checklist for optimization and validation of real-time PCR assays. | Raymaekers M et al. | โ | 2009 | โ |
| Clinical and immunological correlates of pre-co-seasonal sublingual immunotherapy with birch monomeric allergoid in patients with allergic rhinoconjunctivitis. | Burastero SE et al. | โ | 2009 | โ |
| Colorimetric split G-quadruplex probes for nucleic acid sensing: improving reconstituted DNAzyme's catalytic efficiency via probe remodeling. | Nakayama S et al. | โ | 2009 | โ |
| Common variants in the NLRP3 region contribute to Crohn's disease susceptibility. | Villani AC et al. | โ | 2009 | โ |
| Comparative assessment of human and farm animal faecal microbiota using real-time quantitative PCR. | Furet JP et al. | โ | 2009 | โ |
| Developmental validation of the Quantifiler Duo DNA Quantification kit for simultaneous quantification of total human and human male DNA and detection of PCR inhibitors in biological samples. | Barbisin M et al. | โ | 2009 | โ |
| Development and application of genotyping technologies. | Shi J et al. | โ | 2009 | โ |
| DNA sequence detection using surface-enhanced resonance Raman spectroscopy in a homogeneous multiplexed assay. | MacAskill A et al. | โ | 2009 | โ |
| Enhanced Expression of Radiation-induced Leukocyte CDKN1A mRNA in Multiple Primary Breast Cancer Patients: Potential New Marker of Cancer Susceptibility. | Mitsuhashi M et al. | โ | 2009 | โ |
| Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens. | Yang GP et al. | โ | 2009 | โ |
| Familial adenomatous polyposis. | Macrae F et al. | โ | 2009 | โ |
| Fluorescent assay of DNA hybridization with label-free molecular switch: reducing background-signal and improving specificity by using carbon nanotubes. | Liu Y et al. | โ | 2009 | โ |
| General FRET-based coding for application in multiplexing methods. | Giestas L et al. | โ | 2009 | โ |
| Genetic variation in the familial Mediterranean fever gene (MEFV) and risk for Crohn's disease and ulcerative colitis. | Villani AC et al. | โ | 2009 | โ |
| Genotyping of celiac disease-related-risk haplotypes using a closed-tube polymerase chain reaction analysis of dried blood and saliva disk samples. | Ollikka P et al. | โ | 2009 | โ |
| High density labeling of polymerase chain reaction products with the fluorescent base analogue tCo. | Stengel G et al. | โ | 2009 | โ |
| Hydroxylamine-amplified gold nanoparticles for the naked eye and chemiluminescent detection of sequence-specific DNA with notable potential for single-nucleotide polymorphism discrimination. | Fan A et al. | โ | 2009 | โ |
| Identification of regulatory T cells during experimental Leishmania infantum infection. | Rodrigues OR et al. | โ | 2009 | โ |
| Isolation and evaluation of novel adeno-associated virus sequences from porcine tissues. | Bello A et al. | โ | 2009 | โ |
| Macrolide antibiotics inhibit respiratory syncytial virus infection in human airway epithelial cells. | Asada M et al. | โ | 2009 | โ |
| MicroRNA expression changes in lymphoblastoid cell lines in response to lithium treatment. | Chen H et al. | โ | 2009 | โ |
| Molecular engineering of DNA: molecular beacons. | Wang K et al. | โ | 2009 | โ |
| Neuroprotective effects of the triterpenoid, CDDO methyl amide, a potent inducer of Nrf2-mediated transcription. | Yang L et al. | โ | 2009 | โ |
| PCR primer design. | Apte A et al. | โ | 2009 | โ |
| Polyfluorophore labels on DNA: dramatic sequence dependence of quenching. | Teo YN et al. | โ | 2009 | โ |
| Qualitative and quantitative detection of Chlamydophila pneumoniae DNA in cerebrospinal fluid from multiple sclerosis patients and controls. | Tang YW et al. | โ | 2009 | โ |
| Quantitative detection of PML-RARalpha fusion transcript by real-time PCR with a single primer pair. | Takenokuchi M et al. | โ | 2009 | โ |
| Quantitative multiplex PCR assay for the detection of the seven clinically most relevant high-risk HPV types. | Schmitz M et al. | โ | 2009 | โ |
| Quantitative plasma DNA analysis in Parkinson's disease. | Scalzo PL et al. | โ | 2009 | โ |
| Real-time electrochemical monitoring of the polymerase chain reaction by mediated redox catalysis. | Defรฉver T et al. | โ | 2009 | โ |
| Real-time PCR analysis of HIV-1 replication post-entry events. | Mbisa JL et al. | โ | 2009 | โ |
| Real-time PCR assay for rapid detection and quantification of Vibrio aestuarianus in oyster and seawater: a useful tool for epidemiologic studies. | Saulnier D et al. | โ | 2009 | โ |
| Real-time quantification of wild-type contaminants in glyphosate tolerant soybean. | Battistini E et al. | โ | 2009 | โ |
| Relationship of differential gene expression profiles in CD34+ myelodysplastic syndrome marrow cells to disease subtype and progression. | Sridhar K et al. | โ | 2009 | โ |
| Relative quantity of cerebrospinal fluid herpes simplex virus DNA in adult cases of encephalitis and meningitis. | Murphy RF et al. | โ | 2009 | โ |
| Six diagnostic single nucleotide polymorphism markers for detecting introgression between cutthroat and rainbow trouts. | Finger AJ et al. | โ | 2009 | โ |
| SNP genotyping by the 5'-nuclease reaction: advances in high-throughput genotyping with nonmodel organisms. | Seeb JE et al. | โ | 2009 | โ |
| Specific and sensitive detection of Ralstonia solanacearum in soil with quantitative, real-time PCR assays. | Huang J et al. | โ | 2009 | โ |
| Subspecies-informative SNP assays for evaluating introgression between native golden trout and introduced rainbow trout. | Stephens MR et al. | โ | 2009 | โ |
| Surface-enhanced Raman scattering as a detection technique for molecular diagnostics. | Graham D et al. | โ | 2009 | โ |
| Technologies in the Whole-Genome Age: MALDI-TOF-Based Genotyping. | Vogel N et al. | โ | 2009 | โ |
| The beginnings of real-time PCR. | Williams PM | โ | 2009 | โ |
| The optimization of TaqMan real-time RT-PCR assay for transcriptional profiling of GABA-A receptor subunit plasticity. | Gangisetty O et al. | โ | 2009 | โ |
| The TaqMan method for SNP genotyping. | Shen GQ et al. | โ | 2009 | โ |
| Two types of human malignant melanoma cell lines revealed by expression patterns of mitochondrial and survival-apoptosis genes: implications for malignant melanoma therapy. | Su DM et al. | โ | 2009 | โ |
| Absolute quantitation of infectious salmon anaemia virus using different real-time reverse transcription PCR chemistries. | Workenhe ST et al. | โ | 2008 | โ |
| A comprehensive collection of experimentally validated primers for Polymerase Chain Reaction quantitation of murine transcript abundance. | Spandidos A et al. | โ | 2008 | โ |
| A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids. | Yang L et al. | โ | 2008 | โ |
| Carbon nanotube-quenched fluorescent oligonucleotides: probes that fluoresce upon hybridization. | Yang R et al. | โ | 2008 | โ |
| Chromosome 17: association of a large inversion polymorphism with corticosteroid response in asthma. | Tantisira KG et al. | โ | 2008 | โ |
| Comparison of low-density arrays, RT-PCR and real-time TaqMan RT-PCR in detection of grapevine viruses. | Osman F et al. | โ | 2008 | โ |
| Detection of Neospora caninum in dog organs using real time PCR systems. | Ghalmi F et al. | โ | 2008 | โ |
| Determination of DNA polymerase and nuclease activities of DNA-dependent polymerases using fluorescence detection under real-time conditions. | Bragin AG et al. | โ | 2008 | โ |
| Development of a quantitative real-time TaqMan PCR assay for testing the susceptibility of feline herpesvirus-1 to antiviral compounds. | Hussein IT et al. | โ | 2008 | โ |
| Development of a sensitive and quantitative assay for spring viremia of carp virus based on real-time RT-PCR. | Yue Z et al. | โ | 2008 | โ |
| Development of bioluminescent pyrophosphate assay using pyruvate phosphate dikinase and its application to single-nucleotide polymorphism analysis. | Arakawa H et al. | โ | 2008 | โ |
| Development of isothermal TaqMan assays for detection of biothreat organisms. | Tong Y et al. | โ | 2008 | โ |
| DNA-dependent DNA polymerases. | Kucera RB et al. | โ | 2008 | โ |
| DNA hybridization detection in a microfluidic channel using two fluorescently labelled nucleic acid probes. | Chen L et al. | โ | 2008 | โ |
| DNA labeling by ligand inducible secondary structure. | Peng T et al. | โ | 2008 | โ |
| Dysregulated mitochondrial genes and networks with drug targets in postmortem brain of patients with posttraumatic stress disorder (PTSD) revealed by human mitochondria-focused cDNA microarrays. | Su YA et al. | โ | 2008 | โ |
| Effect of sublingual immunotherapy with grass monomeric allergoid on allergen-specific T-cell proliferation and interleukin 10 production. | Burastero SE et al. | โ | 2008 | โ |
| Electrochemical detection of point mutation based on surface ligation reaction and biometallization. | Zhang P et al. | โ | 2008 | โ |
| Enhanced expression of type 1 helper T-cell cytokines in the myocardium of active cardiac sarcoidosis. | Terasaki F et al. | โ | 2008 | โ |
| Ex vivo induction of mRNA in human whole blood as a new platform of drug and dietary supplement development. | Mitsuhashi M et al. | โ | 2008 | โ |
| FIT-probes in real-time PCR. | Socher E et al. | โ | 2008 | โ |
| FIT probes: peptide nucleic acid probes with a fluorescent base surrogate enable real-time DNA quantification and single nucleotide polymorphism discovery. | Socher E et al. | โ | 2008 | โ |
| Genetic variants associated with insulin resistance and metabolic syndrome in young Asian Indians with myocardial infarction. | Ranjith N et al. | โ | 2008 | โ |
| Genotyping with real-time PCR reveals recessive epistasis between independent QTL conferring resistance to common bacterial blight in dry bean. | Vandemark GJ et al. | โ | 2008 | โ |
| Hemodialysis reduces the viral load in uremic patients with chronic hepatitis B infection. | Tseng GY et al. | โ | 2008 | โ |
| Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling. | Hay DC et al. | โ | 2008 | โ |
| High temperature-induced abscisic acid biosynthesis and its role in the inhibition of gibberellin action in Arabidopsis seeds. | Toh S et al. | โ | 2008 | โ |
| High-throughput avian molecular sexing by SYBR green-based real-time PCR combined with melting curve analysis. | Chang HW et al. | โ | 2008 | โ |
| Identification of a chromosome 8p locus for early-onset coronary heart disease in a French Canadian population. | Engert JC et al. | โ | 2008 | โ |
| Inhibitory effects of atrazine on Chlorella vulgaris as assessed by real-time polymerase chain reaction. | Qian H et al. | โ | 2008 | โ |
| In-house HIV-1 RNA real-time RT-PCR assays: principle, available tests and usefulness in developing countries. | Rouet F et al. | โ | 2008 | โ |
| Junction probes - sequence specific detection of nucleic acids via template enhanced hybridization processes. | Nakayama S et al. | โ | 2008 | โ |
| Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. | Tomita N et al. | โ | 2008 | โ |
| Manganese superoxide dismutase (MnSOD) gene polymorphism, interactions with carotenoid levels and prostate cancer risk. | Mikhak B et al. | โ | 2008 | โ |
| MMP-9 haplotypes and carotid artery atherosclerosis: an association study introducing a novel multicolour multiplex RealTime PCR protocol. | Rauch I et al. | โ | 2008 | โ |
| Molecular mechanism underlying differential apoptosis between human melanoma cell lines UACC903 and UACC903(+6) revealed by mitochondria-focused cDNA microarrays. | Zhang Q et al. | โ | 2008 | โ |
| Molecular probes: insights into design and analysis from computational and physical chemistry. | Mitchell FL et al. | โ | 2008 | โ |
| Noncovalent assembly of carbon nanotubes and single-stranded DNA: an effective sensing platform for probing biomolecular interactions. | Yang R et al. | โ | 2008 | โ |
| [Performance evaluation of Abbott RealTime HBV Quantification Kit for HBV viral load by real-time PCR]. | Kim MH et al. | โ | 2008 | โ |
| Plasma DNA, microsatellite alterations, and p53 tumor mutations are associated with disease-free survival in radically resected non-small cell lung cancer patients: a study of the perugia multidisciplinary team for thoracic oncology. | Ludovini V et al. | โ | 2008 | โ |
| Quantification of endogenous and exogenous feline leukemia virus sequences by real-time PCR assays. | Tandon R et al. | โ | 2008 | โ |
| Quantitative promoter methylation analysis of hepatocellular carcinoma, cirrhotic and normal liver. | Harder J et al. | โ | 2008 | โ |
| Quantitative SERRS for DNA sequence analysis. | Graham D et al. | โ | 2008 | โ |
| Quenching of the long-lived Ru(II)bathophenanthroline luminescence for the detection of supramolecular interactions. | Kramer RA et al. | โ | 2008 | โ |
| Real-time PCR and microscopy: are the two methods measuring the same unit of arbuscular mycorrhizal fungal abundance? | Gamper HA et al. | โ | 2008 | โ |
| Real-time PCR for the quantitation of Tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci. | Mason G et al. | โ | 2008 | โ |
| Real-time quantitative polymerase chain reaction for virus infection diagnostics. | Saijo M et al. | โ | 2008 | โ |
| RNA expression analysis from formalin fixed paraffin embedded tissues. | Farragher SM et al. | โ | 2008 | โ |
| SERS-melting: a new method for discriminating mutations in DNA sequences. | Mahajan S et al. | โ | 2008 | โ |
| Sex-stratified linkage analysis identifies a female-specific locus for IgE to cockroach in Costa Ricans. | Hunninghake GM et al. | โ | 2008 | โ |
| The importance of and a method for including transfection efficiency into real-time PCR data analyses. | Godbey WT et al. | โ | 2008 | โ |
| The three-dimensional cultivation of the carcinoma cell line HepG2 in a perfused chip system leads to a more differentiated phenotype of the cells compared to monolayer culture. | Altmann B et al. | โ | 2008 | โ |
| The use of molecular techniques based on ribosomal RNA and DNA for rumen microbial ecosystem studies: a review. | Deng W et al. | โ | 2008 | โ |
| Type A influenza virus detection and quantitation by real-time RT-PCR. | Spackman E et al. | โ | 2008 | โ |
| Affordable assays for genotyping single nucleotide polymorphisms in insects. | Black WC et al. | โ | 2007 | โ |
| A genotype-independent real-time PCR assay for quantification of hepatitis B virus DNA. | Liu Y et al. | โ | 2007 | โ |
| Anti-primer quenching-based real-time PCR for simplex or multiplex DNA quantification and single-nucleotide polymorphism genotyping. | Li J et al. | โ | 2007 | โ |
| A real-time PCR assay for the rapid determination of 16S rRNA genotype in Vibrio vulnificus. | Vickery MC et al. | โ | 2007 | โ |
| Arrayed identification of DNA signatures. | Kรคller M et al. | โ | 2007 | โ |
| A second major histocompatibility complex susceptibility locus for multiple sclerosis. | Yeo TW et al. | โ | 2007 | โ |
| Assessing heteroplasmic load in Leber's hereditary optic neuropathy mutation 3460G->A/MT-ND1 with a real-time PCR quantitative approach. | Genasetti A et al. | โ | 2007 | โ |
| A TaqMan polymerase chain reaction method for monitoring RDX-degrading bacteria based on the xplA functional gene. | Indest KJ et al. | โ | 2007 | โ |
| A targeted deletion/insertion in the mouse Pcsk1 locus is associated with homozygous embryo preimplantation lethality, mutant allele preferential transmission and heterozygous female susceptibility to dietary fat. | Mbikay M et al. | โ | 2007 | โ |
| Automated single-nucleotide polymorphism analysis using fluorescence excitation-emission spectroscopy and one-class classifiers. | Xu Y et al. | โ | 2007 | โ |
| B-DNA helix stability in a solvent-free environment. | Baker ES et al. | โ | 2007 | โ |
| Circulating DNA: a potential marker of sickle cell crisis. | Vasavda N et al. | โ | 2007 | โ |
| Cloning and expression analysis of p26 gene in Artemia sinica. | Jiang L et al. | โ | 2007 | โ |
| Comparative evaluation of the automated Roche TaqMan real-time quantitative human immunodeficiency virus type 1 RNA PCR assay and the Roche AMPLICOR Version 1.5 conventional PCR assay. | Oliver AR et al. | โ | 2007 | โ |
| Comparison of plasma beta-globin DNA and S-100 protein concentrations in acute stroke. | Rainer TH et al. | โ | 2007 | โ |
| Comparison of real-time PCR detection chemistries and cycling modes using Mon810 event-specific assays as model. | La Paz JL et al. | โ | 2007 | โ |
| Comparison of the Roche COBAS Amplicor Monitor, Roche COBAS Ampliprep/COBAS Taqman and Abbott RealTime Test assays for quantification of hepatitis C virus and HIV RNA. | Wolff D et al. | โ | 2007 | โ |
| Comprehensive genetic variant discovery in the surfactant protein B gene. | Hamvas A et al. | โ | 2007 | โ |
| Design and characterization of two-dye and three-dye binary fluorescent probes for mRNA detection. | Martรญ AA et al. | โ | 2007 | โ |
| Detection of sesame seed DNA in foods using real-time PCR. | Brzezinski JL | โ | 2007 | โ |
| Detection of Xanthomonas fragariae and presumptive detection of Xanthomonas arboricola pv. fragariae, from strawberry leaves, by real-time PCR. | Weller SA et al. | โ | 2007 | โ |
| Determination of the ex vivo rates of human immunodeficiency virus type 1 reverse transcription by using novel strand-specific amplification analysis. | Thomas DC et al. | โ | 2007 | โ |
| Diagnostic methods for detection of Classical swine fever virus--status quo and new developments. | Greiser-Wilke I et al. | โ | 2007 | โ |
| Distinct roles for the 5' and 3' untranslated regions in the degradation and accumulation of chloroplast tufA mRNA: identification of an early intermediate in the in vivo degradation pathway. | Zicker AA et al. | โ | 2007 | โ |
| DNA-probes for the highly sensitive identification of single nucleotide polymorphism using single-molecule spectroscopy. | Friedrich A et al. | โ | 2007 | โ |
| Efficient quenching of oligomeric fluorophores on a DNA backbone. | Wilson JN et al. | โ | 2007 | โ |
| Evaluation of a real-time two-step RT-PCR assay for quantitation of Chronic bee paralysis virus (CBPV) genome in experimentally-infected bee tissues and in life stages of a symptomatic colony. | Blanchard P et al. | โ | 2007 | โ |
| Evaluation of the transcription level of the protein disulfide isomerase in different stages from Ancylostoma caninum with a real-time PCR assay. | Epe C et al. | โ | 2007 | โ |
| Expression pattern of myostatin in gastrocnemius muscle of rats after sciatic nerve crush injury. | Liu M et al. | โ | 2007 | โ |
| Faster, simpler, more-specific methods for improved molecular detection of Phytophthora ramorum in the field. | Tomlinson JA et al. | โ | 2007 | โ |
| From molecular diagnostics to personalized testing. | Finan JE et al. | โ | 2007 | โ |
| Fucosyltransferase 3 polymorphism and atherothrombotic disease in the Framingham Offspring Study. | Djoussรฉ L et al. | โ | 2007 | โ |
| Functional consequences of alteration of N-linked glycosylation sites on the neurokinin 1 receptor. | Tansky MF et al. | โ | 2007 | โ |
| Further development of multiplex single nucleotide polymorphism typing method, the DigiTag2 assay. | Nishida N et al. | โ | 2007 | โ |
| Gene expression profiling of CD34+ cells in patients with the 5q- syndrome. | Boultwood J et al. | โ | 2007 | โ |
| Habitat associations of two entomopathogenic nematodes: a quantitative study using real-time quantitative polymerase chain reactions. | Torr P et al. | โ | 2007 | โ |
| Hochu-ekki-to inhibits rhinovirus infection in human tracheal epithelial cells. | Yamaya M et al. | โ | 2007 | โ |
| Human immunodeficiency virus type 1 RNA Levels in different regions of human brain: quantification using real-time reverse transcriptase-polymerase chain reaction. | Kumar AM et al. | โ | 2007 | โ |
| Identification of depot-specific human fat cell progenitors through distinct expression profiles and developmental gene patterns. | Tchkonia T et al. | โ | 2007 | โ |
| Identification of two GTP-independent alternatively spliced forms of tissue transglutaminase in human leukocytes, vascular smooth muscle, and endothelial cells. | Lai TS et al. | โ | 2007 | โ |
| Improved COBAS TaqMan hepatitis C virus test (Version 2.0) for use with the High Pure system: enhanced genotype inclusivity and performance characteristics in a multisite study. | Colucci G et al. | โ | 2007 | โ |
| Increased TNFalpha and CCAAT/enhancer-binding protein homologous protein with aging predispose preadipocytes to resist adipogenesis. | Tchkonia T et al. | โ | 2007 | โ |
| Isolated tumour cells in blood and E-cadherin expression in oesophageal squamous cell cancer. | Setoyama T et al. | โ | 2007 | โ |
| Laboratory diagnosis of CNS infections by molecular amplification techniques. | Tang YW | โ | 2007 | โ |
| Microfluidic control of fluorescence resonance energy transfer: breaking the FRET limit. | Zhang CY et al. | โ | 2007 | โ |
| Multidrug resistance protein expression of adult T-cell leukemia/lymphoma. | Yasunami T et al. | โ | 2007 | โ |
| New FRET primers for quantitative real-time PCR. | Ahmad AI et al. | โ | 2007 | โ |
| Noninvasive fetal RHD genotyping from maternal plasma. Use of a new developed Free DNA Fetal Kit RhD. | Rouillac-Le Sciellour C et al. | โ | 2007 | โ |
| Novel DNA probes with low background and high hybridization-triggered fluorescence. | Lukhtanov EA et al. | โ | 2007 | โ |
| Optimization of fluorescence measurement in duplex real-time PCR with TaqMan probes labeled with VIC and quenched by TAMRA. | Kuchta T et al. | โ | 2007 | โ |
| Pharmacogenetic screening of the gene deletion and duplications of CYP2D6. | Meijerman I et al. | โ | 2007 | โ |
| Polymorphisms in IL13, total IgE, eosinophilia, and asthma exacerbations in childhood. | Hunninghake GM et al. | โ | 2007 | โ |
| Q-priming PCR: a quantitative real-time PCR system using a self-quenched BODIPY FL-labeled primer. | Liu XK et al. | โ | 2007 | โ |
| Quantification of uncultured microorganisms by fluorescence microscopy and digital image analysis. | Daims H et al. | โ | 2007 | โ |
| Quantitative analysis of chromosome conformation capture assays (3C-qPCR). | Hagรจge H et al. | โ | 2007 | โ |
| Quantitative real-time PCR for rapid and accurate titration of recombinant baculovirus particles. | Hitchman RB et al. | โ | 2007 | โ |
| Real-time PCR for determining capsular serotypes of Haemophilus influenzae. | Maaroufi Y et al. | โ | 2007 | โ |
| Real-time PCR to identify variola virus or other human pathogenic orthopox viruses. | Scaramozzino N et al. | โ | 2007 | โ |
| Real-time RT-PCR (TaqMan) assays for the detection of Grapevine Leafroll associated viruses 1-5 and 9. | Osman F et al. | โ | 2007 | โ |
| Real-time TaqMan polymerase chain reaction assays for quantitative detection and differentiation of Ureaplasma urealyticum and Ureaplasma parvum. | Cao X et al. | โ | 2007 | โ |
| Role of law enforcement response and microbial forensics in investigation of bioterrorism. | Budowle B et al. | โ | 2007 | โ |
| Role of plasma DNA as a predictive marker of fatal outcome following severe head injury in males. | Campello Yurgel V et al. | โ | 2007 | โ |
| Satellite DNA as a target for TaqMan real-time PCR detection of the pinewood nematode, Bursaphelenchus xylophilus. | Franรงois C et al. | โ | 2007 | โ |
| Serial processing of biological reactions using flow-through microfluidic devices: coupled PCR/LDR for the detection of low-abundant DNA point mutations. | Hashimoto M et al. | โ | 2007 | โ |
| Serum response factor and myocardin mediate arterial hypercontractility and cerebral blood flow dysregulation in Alzheimer's phenotype. | Chow N et al. | โ | 2007 | โ |
| Simultaneous detection of potato viruses, PLRV, PVA, PVX and PVY from dormant potato tubers by TaqMan real-time RT-PCR. | Agindotan BO et al. | โ | 2007 | โ |
| Single-molecule measurements with a single quantum dot. | Kaji N et al. | โ | 2007 | โ |
| SNP and mutation analysis. | Wang L et al. | โ | 2007 | โ |
| SNP genotyping: technologies and biomedical applications. | Kim S et al. | โ | 2007 | โ |
| Tentacle probes: eliminating false positives without sacrificing sensitivity. | Satterfield BC et al. | โ | 2007 | โ |
| The diagnosis of genital herpes - beyond culture: An evidence-based guide for the utilization of polymerase chain reaction and herpes simplex virus type-specific serology. | Ratnam S et al. | โ | 2007 | โ |
| The evolving role of drug metabolism in drug discovery and development. | Yengi LG et al. | โ | 2007 | โ |
| Thermodynamically modulated partially double-stranded linear DNA probe design for homogeneous real-time PCR. | Huang S et al. | โ | 2007 | โ |
| Third-generation human mitochondria-focused cDNA microarray and its bioinformatic tools for analysis of gene expression. | Bai X et al. | โ | 2007 | โ |
| Time-resolved detection probe for homogeneous nucleic acid analyses in one-step format. | Laitala V et al. | โ | 2007 | โ |
| Validation of reduced-scale reactions for the Quantifiler Human DNA kit. | Westring CG et al. | โ | 2007 | โ |
| Abundance and distribution of Ostreococcus sp. in the San Pedro Channel, California, as revealed by quantitative PCR. | Countway PD et al. | โ | 2006 | โ |
| Advanced techniques in the diagnosis and management of infectious pulmonary diseases in horses. | Brown VA et al. | โ | 2006 | โ |
| Advances in molecular phytodiagnostics - new solutions for old problems. | Mumford R et al. | โ | 2006 | โ |
| Affinity capillary electrophoresis of DNA for detection of single-nucleotide polymorphisms and point mutations. Comprehensive study for optimization of the weak affinity. | Sato K et al. | โ | 2006 | โ |
| A Multiplex Real-Time Polymerase Chain Reaction (TaqMan) Assay for the Simultaneous Detection of Meloidogyne chitwoodi and M. fallax. | Zijlstra C et al. | โ | 2006 | โ |
| A new approach for DNA detection by SERRS. | Faulds K et al. | โ | 2006 | โ |
| A novel endonuclease IV post-PCR genotyping system. | Kutyavin IV et al. | โ | 2006 | โ |
| Antiprimer quenching-based real-time PCR and its application to the analysis of clinical cancer samples. | Li J et al. | โ | 2006 | โ |
| Applications of real-time polymerase chain reaction for quantification of microorganisms in environmental samples. | Zhang T et al. | โ | 2006 | โ |
| Assessment of real-time PCR based methods for quantification of pollen-mediated gene flow from GM to conventional maize in a field study. | Pla M et al. | โ | 2006 | โ |
| Carbocisteine inhibits rhinovirus infection in human tracheal epithelial cells. | Yasuda H et al. | โ | 2006 | โ |
| Carcinoembryonic antigen messenger RNA expression in blood predicts recurrence in esophageal cancer. | Setoyama T et al. | โ | 2006 | โ |
| Characterization of a Novel Adhesin-like Gene and Design of a Real-Time PCR for Rapid, Sensitive, and Specific Detection of Spiroplasma kunkelii. | Wei W et al. | โ | 2006 | โ |
| Common laboratory methods in pharmacogenomics studies. | Aquilante CL et al. | โ | 2006 | โ |
| [Comparison between Real-Time PCR and Agarose Gel Electrophoresis for DNA Quantification.]. | Lee MK et al. | โ | 2006 | โ |
| Comparison of methods of extracting Salmonella enterica serovar Enteritidis DNA from environmental substrates and quantification of organisms by using a general internal procedural control. | Klerks MM et al. | โ | 2006 | โ |
| Comparison of PrASE and Pyrosequencing for SNP Genotyping. | Kรคller M et al. | โ | 2006 | โ |
| Comparison of synthetic DNA templates with authentic cDNA templates in terms of quantification by real-time quantitative reverse transcription polymerase chain reaction. | Moriya Y et al. | โ | 2006 | โ |
| Correlation of the expression levels of BLyS and its receptors mRNA in patients with systemic lupus erythematosus. | Ju S et al. | โ | 2006 | โ |
| Defects in surfactant synthesis: clinical implications. | Cole FS et al. | โ | 2006 | โ |
| Detection and monitoring of virus infections by real-time PCR. | Watzinger F et al. | โ | 2006 | โ |
| Detection of biological threat agents by real-time PCR: comparison of assay performance on the R.A.P.I.D., the LightCycler, and the Smart Cycler platforms. | Christensen DR et al. | โ | 2006 | โ |
| Determination of cytoplasmic male sterile factors in onion plants (Allium cepa L.) using PCR-RFLP and SNP markers. | Cho KS et al. | โ | 2006 | โ |
| Development of a human-specific real-time PCR assay for the simultaneous quantitation of total genomic and male DNA. | Horsman KM et al. | โ | 2006 | โ |
| Early detection of Biscogniauxia nummularia in symptomless European beech (Fagus sylvatica L.) by TaqMan quantitative real-time PCR. | Luchi N et al. | โ | 2006 | โ |
| Early detection of plasma cytomegalovirus DNA by real-time PCR after allogeneic hematopoietic stem cell transplantation. | Onishi Y et al. | โ | 2006 | โ |
| Eotaxin polymorphisms and serum total IgE levels in children with asthma. | Raby BA et al. | โ | 2006 | โ |
| Equal performance of TaqMan, MGB, molecular beacon, and SYBR green-based detection assays in detection and quantification of roundup ready soybean. | Andersen CB et al. | โ | 2006 | โ |
| Establishment and application of a TaqMan real-time quantitative reverse transcription-polymerase chain reaction assay for rubella virus RNA. | Zhao LH et al. | โ | 2006 | โ |
| Evaluation of quantitative polymerase chain reaction-based approaches for determining gene copy and gene transcript numbers in environmental samples. | Smith CJ et al. | โ | 2006 | โ |
| Evaluation of real-time PCR amplification efficiencies to detect PCR inhibitors. | Kontanis EJ et al. | โ | 2006 | โ |
| Expression of myostatin RNA transcript and protein in gastrocnemius muscle of rats after sciatic nerve resection. | Zhang D et al. | โ | 2006 | โ |
| Four-base codon mediated mRNA display to construct peptide libraries that contain multiple nonnatural amino acids. | Muranaka N et al. | โ | 2006 | โ |
| Genetic association analysis of functional impairment in chronic obstructive pulmonary disease. | Hersh CP et al. | โ | 2006 | โ |
| Genomics basics: DNA structure, gene expression, cloning, genetic mapping, and molecular tests. | Tefferi A | โ | 2006 | โ |
| H-2 complex influences cytokine gene expression in Leishmania infantum-infected macrophages. | Rodrigues OR et al. | โ | 2006 | โ |
| Heated oligonucleotide ligation assay (HOLA): an affordable single nucleotide polymorphism assay. | Black WC et al. | โ | 2006 | โ |
| High-throughput single-nucleotide polymorphisms genotyping: TaqMan assay and pyrosequencing assay. | Shen GQ et al. | โ | 2006 | โ |
| [HTLV-1 and HTLV-2 proviral load: a simple method using quantitative real-time PCR]. | Tamegรฃo-Lopes BP et al. | โ | 2006 | โ |
| Identification of single-point mutations in mycobacterial 16S rRNA sequences by confocal single-molecule fluorescence spectroscopy. | Marmรฉ N et al. | โ | 2006 | โ |
| Induction of colitis causes inflammatory responses in fat depots: evidence for substance P pathways in human mesenteric preadipocytes. | Karagiannides I et al. | โ | 2006 | โ |
| Influence of cyclic hydrostatic pressure on fibrocartilaginous metaplasia of achilles tendon fibroblasts. | Shim JW et al. | โ | 2006 | โ |
| Locked nucleic acids for optimizing displacement probes for quantitative real-time PCR. | Kennedy B et al. | โ | 2006 | โ |
| Matrix-assisted laser desorption/ionization mass spectrometric analysis of DNA on microarrays. | Kepper P et al. | โ | 2006 | โ |
| Multicenter performance evaluation of a new TaqMan PCR assay for monitoring human immunodeficiency virus RNA load. | Perrin L et al. | โ | 2006 | โ |
| Multiplexed detection of anthrax-related toxin genes. | Moser MJ et al. | โ | 2006 | โ |
| No association between selected candidate gene polymorphisms and severe chronic periodontitis. | Wohlfahrt JC et al. | โ | 2006 | โ |
| Non-cross-linking gold nanoparticle aggregation for sensitive detection of single-nucleotide polymorphisms: optimization of the particle diameter. | Sato K et al. | โ | 2006 | โ |
| Nuclear and mitochondrial DNA quantification of various forensic materials. | Andrรฉasson H et al. | โ | 2006 | โ |
| Nucleic acid testing for viral burden and viral genotyping. | Domiati-Saad R et al. | โ | 2006 | โ |
| One-step homogeneous detection of DNA hybridization with gold nanoparticle probes by using a linear light-scattering technique. | Du BA et al. | โ | 2006 | โ |
| [Performance evaluation of real-q HBV quantification kit for HBV DNA by real-time PCR.]. | Hwang SH et al. | โ | 2006 | โ |
| Phylogenetic relationship of psychoactive fungi based on rRNA gene for a large subunit and their identification using the TaqMan assay (II). | Maruyama T et al. | โ | 2006 | โ |
| PNA/dsDNA complexes: site specific binding and dsDNA biosensor applications. | Baker ES et al. | โ | 2006 | โ |
| Polymerase chain reaction/ligase detection reaction/hybridization assays using flow-through microfluidic devices for the detection of low-abundant DNA point mutations. | Hashimoto M et al. | โ | 2006 | โ |
| Present and future of rapid and/or high-throughput methods for nucleic acid testing. | Csako G | โ | 2006 | โ |
| Quantification of chemokines by real-time reverse transcriptase PCR: applications in type 1 diabetes. | Overbergh L et al. | โ | 2006 | โ |
| Quantification of mRNA in whole blood by assessing recovery of RNA and efficiency of cDNA synthesis. | Mitsuhashi M et al. | โ | 2006 | โ |
| Quantitation of hepatitis B virus DNA by real-time PCR using internal amplification control and dual TaqMan MGB probes. | Lole KS et al. | โ | 2006 | โ |
| RAPHIDOPHYCEAE [CHADEFAUD EX SILVA] SYSTEMATICS AND RAPID IDENTIFICATION: SEQUENCE ANALYSES AND REAL-TIME PCR ASSAYS. | Bowers HA et al. | โ | 2006 | โ |
| Rapid detection, serotyping and quantitation of dengue viruses by TaqMan real-time one-step RT-PCR. | Kong YY et al. | โ | 2006 | โ |
| Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA. | Lu YQ et al. | โ | 2006 | โ |
| Real-time assays with molecular beacons and other fluorescent nucleic acid hybridization probes. | Marras SA et al. | โ | 2006 | โ |
| Real-Time Fluorescent Polymerase Chain Reaction Detection of Phytophthora ramorum and Phytophthora pseudosyringae Using Mitochondrial Gene Regions. | Tooley PW et al. | โ | 2006 | โ |
| Real-time fluorogenic reverse transcription-PCR assays for detection of bacteriophage MS2. | O'Connell KP et al. | โ | 2006 | โ |
| Real-time PCR detection and quantification of vector trichodorid nematodes and Tobacco rattle virus. | Holeva R et al. | โ | 2006 | โ |
| Real-time polymerase chain reaction: a novel molecular diagnostic tool for equine infectious diseases. | Pusterla N et al. | โ | 2006 | โ |
| Real-time quantitative PCR assays for quantification of L1781 ACCase inhibitor resistance allele in leaf and seed pools of Lolium populations. | Kaundun SS et al. | โ | 2006 | โ |
| Relations of APOE promoter polymorphisms to LDL cholesterol and markers of subclinical atherosclerosis in young adults. | Viiri LE et al. | โ | 2006 | โ |
| Sterol regulatory element-binding protein 1 mediates liver X receptor-beta-induced increases in insulin secretion and insulin messenger ribonucleic acid levels. | Zitzer H et al. | โ | 2006 | โ |
| Suppression of Mycobacterium tuberculosis induced reactive oxygen species (ROS) and TNF-alpha mRNA expression in human monocytes by allicin. | Hasan N et al. | โ | 2006 | โ |
| Surface plasmon resonance imaging on a microchip for detection of DNA-modified gold nanoparticles deposited onto the surface in a non-cross-linking configuration. | Sato Y et al. | โ | 2006 | โ |
| Targeted gene-expression analysis by genome-controlled reverse transcription-PCR. | Stenman J et al. | โ | 2006 | โ |
| T-bet polymorphisms are associated with asthma and airway hyperresponsiveness. | Raby BA et al. | โ | 2006 | โ |
| The association of prothrombin A19911G polymorphism with plasma prothrombin activity and venous thrombosis: results of the MEGA study, a large population-based case-control study. | Chinthammitr Y et al. | โ | 2006 | โ |
| The real-time polymerase chain reaction. | Kubista M et al. | โ | 2006 | โ |
| The use of real-time PCR methods in DNA sequence variation analysis. | Gibson NJ | โ | 2006 | โ |
| Towards computing with proteins. | Unger R et al. | โ | 2006 | โ |
| Typing of single nucleotide polymorphisms by MALDI mass spectrometry: principles and diagnostic applications. | Sauer S | โ | 2006 | โ |
| Variable populations within variable populations: quantifying mitochondrial heteroplasmy in natural populations of the gynodioecious plant Silene vulgaris. | Welch ME et al. | โ | 2006 | โ |
| Adenovirus infection after pediatric bone marrow transplantation: is treatment always necessary? | Walls T et al. | โ | 2005 | โ |
| Advances in real-time PCR: application to clinical laboratory diagnostics. | Kaltenboeck B et al. | โ | 2005 | โ |
| A method for determining zygosity of transgenic zebrafish by TaqMan real-time PCR. | Ji W et al. | โ | 2005 | โ |
| An examination of MS candidate genes identified as differentially regulated in multiple sclerosis plaque tissue, using absolute and comparative real-time Q-PCR analysis. | Tajouri L et al. | โ | 2005 | โ |
| A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs. | Decaro N et al. | โ | 2005 | โ |
| Attempted replication of reported chronic obstructive pulmonary disease candidate gene associations. | Hersh CP et al. | โ | 2005 | โ |
| Basic principles of real-time quantitative PCR. | Arya M et al. | โ | 2005 | โ |
| BCRABL transcript detection by quantitative real-time PCR : are correlated results possible from homebrew assays? | Fossey SC et al. | โ | 2005 | โ |
| Changes in gene expression profiles of multiple myeloma cells induced by arsenic trioxide (ATO): possible mechanisms to explain ATO resistance in vivo. | Zhou P et al. | โ | 2005 | โ |
| Characterization of virus isolates by particle-associated nucleic acid PCR. | Stang A et al. | โ | 2005 | โ |
| Chemokine gene expression during allograft rejection: comparison of two quantitative PCR techniques. | Carvalho-Gaspar M et al. | โ | 2005 | โ |
| Cis acting expression loci in multiple sclerosis. | Ban M et al. | โ | 2005 | โ |
| Comprehensive algorithm for quantitative real-time polymerase chain reaction. | Zhao S et al. | โ | 2005 | โ |
| Current and developing technologies for monitoring agents of bioterrorism and biowarfare. | Lim DV et al. | โ | 2005 | โ |
| Detection and identification of Candida species associated with Candida vaginitis by real-time PCR and pyrosequencing. | Trama JP et al. | โ | 2005 | โ |
| Detection of the Pinewood Nematode, Bursaphelenchus xylophilus, Using a Real-Time Polymerase Chain Reaction Assay. | Cao AX et al. | โ | 2005 | โ |
| Developmental validation of the quantifiler real-time PCR kits for the quantification of human nuclear DNA samples. | Green RL et al. | โ | 2005 | โ |
| Development of a novel Ag-specific immunotherapy using CpG oligodeoxynucleotides in a new, unique mouse cutaneous eosinophilic inflammation model. | Kootiratrakarn T et al. | โ | 2005 | โ |
| Development of a real-time RT-PCR assay for detection and quantitation of parainfluenza virus 3. | Hu A et al. | โ | 2005 | โ |
| Differentiation between amplicon polymerization and nonspecific products in SYBR green I real-time polymerase chain reaction. | Weng T et al. | โ | 2005 | โ |
| DigiTag assay for multiplex single nucleotide polymorphism typing with high success rate. | Nishida N et al. | โ | 2005 | โ |
| Elevated serum tumor necrosis factor alpha and ferritin may contribute to the insulin resistance found in HCV positive Egyptian patients. | Elsammak M et al. | โ | 2005 | โ |
| Evaluation of a panel of tumor markers for molecular detection of circulating cancer cells in women with suspected breast cancer. | Reinholz MM et al. | โ | 2005 | โ |
| Exploiting molecular methods to explore endodontic infections: Part 1--current molecular technologies for microbiological diagnosis. | Siqueira JF et al. | โ | 2005 | โ |
| Fifty years of molecular (DNA/RNA) diagnostics. | Gingeras TR et al. | โ | 2005 | โ |
| Fluorescence based strategies for genetic analysis. | Ranasinghe RT et al. | โ | 2005 | โ |
| Forced intercalation probes (FIT Probes): thiazole orange as a fluorescent base in peptide nucleic acids for homogeneous single-nucleotide-polymorphism detection. | Kรถhler O et al. | โ | 2005 | โ |
| Four-color multiplex reverse transcription polymerase chain reaction--overcoming its limitations. | Persson K et al. | โ | 2005 | โ |
| Genetic analysis and attribution of microbial forensics evidence. | Budowle B et al. | โ | 2005 | โ |
| Genotyping Common Bean for the Potyvirus Resistance Alleles I and bc-1(2) with a Multiplex Real-Time Polymerase Chain Reaction Assay. | Vandemark GJ et al. | โ | 2005 | โ |
| Genotyping--from genomic DNA to genotype in a single tube. | Strerath M et al. | โ | 2005 | โ |
| Haplotypes of the WNK1 gene associate with blood pressure variation in a severely hypertensive population from the British Genetics of Hypertension study. | Newhouse SJ et al. | โ | 2005 | โ |
| High throughput, nanoliter quantitative PCR. | Brenan C et al. | โ | 2005 | โ |
| High-throughput qualitative multiplex 5' nuclease assay using post-only PCR analysis. | Antonishyn NA et al. | โ | 2005 | โ |
| HLA class II allele control of HPV load in carcinoma in situ of the cervix uteri. | Beskow AH et al. | โ | 2005 | โ |
| Identification and quantitation of species in complex DNA mixtures by real-time polymerase chain reaction. | Lรณpez-Andreo M et al. | โ | 2005 | โ |
| Increased expression of MIP-3alpha/CCL20 in peripheral blood mononuclear cells from patients with ulcerative colitis and its down-regulation by sulfasalazine and glucocorticoid treatment. | Lee HJ et al. | โ | 2005 | โ |
| Influence of alcohol dehydrogenase 1C polymorphism on the alcohol-cardiovascular disease association (from the Framingham Offspring Study). | Djoussรฉ L et al. | โ | 2005 | โ |
| Interfamilial characterization of a region of the ZFX and ZFY genes facilitates sex determination in cetaceans and other mammals. | Morin PA et al. | โ | 2005 | โ |
| KIT overexpression and amplification in gastrointestinal stromal tumors (GISTs). | Tabone S et al. | โ | 2005 | โ |
| Mechanisms of combined treatment with celiprolol and candesartan for ventricular remodeling in experimental heart failure. | Mori T et al. | โ | 2005 | โ |
| Microarray analysis of postictal transcriptional regulation of neuropeptides. | Wilson DN et al. | โ | 2005 | โ |
| Miniaturization in functional genomics and proteomics. | Sauer S et al. | โ | 2005 | โ |
| Molecular assembly of superquenchers in signaling molecular interactions. | Yang CJ et al. | โ | 2005 | โ |
| Molecular basis of action of HyBeacon fluorogenic probes: a spectroscopic and molecular dynamics study. | Marks AH et al. | โ | 2005 | โ |
| Non-cross-linking gold nanoparticle aggregation as a detection method for single-base substitutions. | Sato K et al. | โ | 2005 | โ |
| On-site DNA extraction and real-time PCR for detection of Phytophthora ramorum in the field. | Tomlinson JA et al. | โ | 2005 | โ |
| Overexpression of the TGF-beta antagonist Smad7 in endometrial cancer. | Dowdy SC et al. | โ | 2005 | โ |
| Parameters involved in the conversion of real-time PCR assays from the ABI prism 7700 to the Cepheid SmartCycler II. | Bentley HA et al. | โ | 2005 | โ |
| Phylogenetic analysis and real time PCR detection of a presumbably undescribed Peronospora species on sweet basil and sage. | Belbahri L et al. | โ | 2005 | โ |
| PieceMaker: selection of DNA fragments for selector-guided multiplex amplification. | Stenberg J et al. | โ | 2005 | โ |
| Plasma hydroxy-metronidazole/metronidazole ratio in hepatitis C virus-induced liver disease. | Marchioretto MA et al. | โ | 2005 | โ |
| Prevalence, quantification and typing of adenoviruses detected in river and treated drinking water in South Africa. | van Heerden J et al. | โ | 2005 | โ |
| Prognostic significance of BACH2 expression in diffuse large B-cell lymphoma: a study of the Osaka Lymphoma Study Group. | Sakane-Ishikawa E et al. | โ | 2005 | โ |
| Prognostic significance of CD40 expression in malignant lymphoma developing in rheumatoid arthritis. | Sasaki T et al. | โ | 2005 | โ |
| Protective effect of matrix metalloproteinase inhibitors against epidermal basement membrane damage: skin equivalents partially mimic photoageing process. | Amano S et al. | โ | 2005 | โ |
| Quantifying mixed populations of drug-resistant human immunodeficiency virus type 1. | Moser MJ et al. | โ | 2005 | โ |
| Quantitation of feline leukaemia virus viral and proviral loads by TaqMan real-time polymerase chain reaction. | Tandon R et al. | โ | 2005 | โ |
| Quantitative analysis of laminin 5 gene expression in human keratinocytes. | Akutsu N et al. | โ | 2005 | โ |
| Quantitative analysis of multi-species oral biofilms by TaqMan Real-Time PCR. | Suzuki N et al. | โ | 2005 | โ |
| Quantitative PCR-enhanced immunoassay for measurement of enteroviral immunoglobulin M antibody and diagnosis of aseptic meningitis. | Elfaitouri A et al. | โ | 2005 | โ |
| Quantitative real-time PCR assays for detection of human adenoviruses and identification of serotypes 40 and 41. | Jothikumar N et al. | โ | 2005 | โ |
| Quantitative real-time PCR for cancer detection: the lymphoma case. | Stรฅhlberg A et al. | โ | 2005 | โ |
| Quantitative real-time reverse transcription-PCR analysis of deformed wing virus infection in the honeybee (Apis mellifera L.). | Chen YP et al. | โ | 2005 | โ |
| Quenching of CdSe quantum dot emission, a new approach for biosensing. | Dyadyusha L et al. | โ | 2005 | โ |
| Real-time PCR detection of Biscogniauxia mediterranea in symptomless oak tissue. | Luchi N et al. | โ | 2005 | โ |
| Real-time PCR for the detection of Escherichia coli O157:H7 in dairy and cattle wastewater. | Spano G et al. | โ | 2005 | โ |
| Real-time polymerase chain reaction for detection and quantification of bacteria in periodontal patients. | Nonnenmacher C et al. | โ | 2005 | โ |
| Real-time polymerase chain reaction quantification of human cytomegalovirus and Epstein-Barr virus in periodontal pockets and the adjacent gingiva of periodontitis lesions. | Kubar A et al. | โ | 2005 | โ |
| Real-Time Polymerase Chain Reaction Quantification of Phytophthora capsici in Different Pepper Genotypes. | Silvar C et al. | โ | 2005 | โ |
| Real-time quantitative PCR analysis of temporal-spatial alterations in gene expression after spinal cord contusion. | Wu X et al. | โ | 2005 | โ |
| Real Time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus. | Boxus M et al. | โ | 2005 | โ |
| Role of the 2 adenine (g.11293_11294insAA) insertion polymorphism in the 3' untranslated region of the factor VII (FVII) gene: molecular characterization of a patient with severe FVII deficiency. | Peyvandi F et al. | โ | 2005 | โ |
| Role of the MEOX2 homeobox gene in neurovascular dysfunction in Alzheimer disease. | Wu Z et al. | โ | 2005 | โ |
| Sequential expression of three known protective genes in cardiac biopsies after transplantation. | Souza AI et al. | โ | 2005 | โ |
| Single nucleotide polymorphisms: discovery, detection and analysis. | Ronaghi M et al. | โ | 2005 | โ |
| Single-quantum-dot-based DNA nanosensor. | Zhang CY et al. | โ | 2005 | โ |
| SNPs in forensic genetics: a review on SNP typing methodologies. | Sobrino B et al. | โ | 2005 | โ |
| Species-specific detection and quantification of toxic marine dinoflagellates Alexandrium tamarense and A. catenella by Real-time PCR assay. | Hosoi-Tanabe S et al. | โ | 2005 | โ |
| Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR. | May JP et al. | โ | 2005 | โ |
| Synthesis of a new pair of fluorescence resonance energy transfer donor and acceptor dyes and its use in a protease assay. | Kainmรผller EK et al. | โ | 2005 | โ |
| Systems biology in drug safety and metabolism: integration of microarray, real-time PCR and enzyme approaches. | Yengi LG | โ | 2005 | โ |
| TaqMan real-time PCR for the detection and quantitation of pork in meat mixtures. | Rodrรญguez MA et al. | โ | 2005 | โ |
| The association of the apolipoprotein E gene promoter polymorphisms and haplotypes with serum lipid and lipoprotein concentrations. | Viiri LE et al. | โ | 2005 | โ |
| The proton pump inhibitor lansoprazole inhibits rhinovirus infection in cultured human tracheal epithelial cells. | Sasaki T et al. | โ | 2005 | โ |
| Tissue-specific transcriptome responses in rats with early streptozotocin-induced diabetes. | Knoll KE et al. | โ | 2005 | โ |
| Topical antisense oligonucleotide therapy against LIX, an enterocyte-expressed CXC chemokine, reduces murine colitis. | Kwon JH et al. | โ | 2005 | โ |
| Transfer and evaluation of an automated, low-cost real-time reverse transcription-PCR test for diagnosis and monitoring of human immunodeficiency virus type 1 infection in a West African resource-limited setting. | Rouet F et al. | โ | 2005 | โ |
| Trends in dengue diagnosis. | Teles FR et al. | โ | 2005 | โ |
| Two for typing: homogeneous combined single-nucleotide polymorphism scanning and genotyping. | von Ahsen N | โ | 2005 | โ |
| Ultraconserved regions in multiple sclerosis. | Ban M et al. | โ | 2005 | โ |
| Update on the laboratory diagnosis and monitoring of HIV infection. | Constantine NT et al. | โ | 2005 | โ |
| Use of sequence data from rainbow trout and Atlantic salmon for SNP detection in Pacific salmon. | Smith CT et al. | โ | 2005 | โ |
| Varicella-zoster virus infection of human dorsal root ganglia in vivo. | Zerboni L et al. | โ | 2005 | โ |
| A comparison between SNaPshot, pyrosequencing, and biplex invader SNP genotyping methods: accuracy, cost, and throughput. | Pati N et al. | โ | 2004 | โ |
| Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. | Yamauchi Y et al. | โ | 2004 | โ |
| A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real-time PCR. | Purfield A et al. | โ | 2004 | โ |
| A real-time PCR assay for DNA-methylation using methylation-specific blockers. | Cottrell SE et al. | โ | 2004 | โ |
| A second-generation anthrax "smoke detector". | Lester ED et al. | โ | 2004 | โ |
| Association of vitamin D receptor gene polymorphisms with childhood and adult asthma. | Raby BA et al. | โ | 2004 | โ |
| Association study of dopamine transporter gene and schizophrenia in Korean population using multiple single nucleotide polymorphism markers. | Jeong SH et al. | โ | 2004 | โ |
| Balanced-PCR amplification allows unbiased identification of genomic copy changes in minute cell and tissue samples. | Wang G et al. | โ | 2004 | โ |
| Changes in aquaporin expression in the inner ear of the rat after i.p. injection of steroids. | Fukushima M et al. | โ | 2004 | โ |
| Characterization and applications of CataCleave probe in real-time detection assays. | Harvey JJ et al. | โ | 2004 | โ |
| Cloning of the gene encoding urease subunit A in Helicobacter pylori. | Shi L et al. | โ | 2004 | โ |
| Combining genome-wide and targeted gene expression profiling in drug discovery: microarrays and real-time PCR. | Brazeau DA | โ | 2004 | โ |
| Common variants in the 5' region of the leptin gene are associated with body mass index in men from the National Heart, Lung, and Blood Institute Family Heart Study. | Jiang Y et al. | โ | 2004 | โ |
| Comparison of plasma Epstein-Barr virus (EBV) DNA levels and serum EBV immunoglobulin A/virus capsid antigen antibody titers in patients with nasopharyngeal carcinoma. | Shao JY et al. | โ | 2004 | โ |
| Comparison of real-time PCR methods for detection of Salmonella enterica and Escherichia coli O157:H7, and introduction of a general internal amplification control. | Klerks MM et al. | โ | 2004 | โ |
| Complex haplotype structure of the human GNAS gene identifies a recombination hotspot centred on a single nucleotide polymorphism widely used in association studies. | Yang W et al. | โ | 2004 | โ |
| Cytomegalovirus infection in organ-transplant recipients: diagnostic value of pp65 antigen test, qualitative polymerase chain reaction (PCR) and quantitative Taqman PCR. | Meyer-Koenig U et al. | โ | 2004 | โ |
| Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement. | Ward CL et al. | โ | 2004 | โ |
| Detection and Quantification of Fusarium solani f. sp. glycines in Soybean Roots with Real-Time Quantitative Polymerase Chain Reaction. | Gao X et al. | โ | 2004 | โ |
| Detection of Australasian Flavivirus encephalitic viruses using rapid fluorogenic TaqMan RT-PCR assays. | Pyke AT et al. | โ | 2004 | โ |
| Detection of genetically modified organisms in foods by DNA amplification techniques. | Garcรญa-Caรฑas V et al. | โ | 2004 | โ |
| Detection of influenza B in clinical specimens: comparison of high throughput RT-PCR and culture confirmation. | Cheng SM et al. | โ | 2004 | โ |
| Detection of mRNA by in situ hybridization and RT-PCR. | Ransick A | โ | 2004 | โ |
| Development and evaluation of an efficient 3'-noncoding region based SARS coronavirus (SARS-CoV) RT-PCR assay for detection of SARS-CoV infections. | Houng HS et al. | โ | 2004 | โ |
| Development of a real-time RT-PCR assay for the detection of potato spindle tuber viroid. | Boonham N et al. | โ | 2004 | โ |
| Differential gene expression of TGF beta inducible early gene (TIEG), Smad7, Smad2 and Bard1 in normal and malignant breast tissue. | Reinholz MM et al. | โ | 2004 | โ |
| Distinct pattern of gene expression in pyothorax-associated lymphoma (PAL), a lymphoma developing in long-standing inflammation. | Nishiu M et al. | โ | 2004 | โ |
| DNA amplification method tolerant to sample degradation. | Wang G et al. | โ | 2004 | โ |
| Dynamic EBV gene loads in renal, hepatic, and cardiothoracic transplant recipients as determined by real-time PCR light cycler. | Leung E et al. | โ | 2004 | โ |
| Elevated expression of valosin-containing protein (p97) is associated with poor prognosis of prostate cancer. | Tsujimoto Y et al. | โ | 2004 | โ |
| Emerging genetic technologies in clinical and research settings. | Cashion AK et al. | โ | 2004 | โ |
| Energy substrate modulates mitochondrial structure and oxidative capacity in cancer cells. | Rossignol R et al. | โ | 2004 | โ |
| Enhanced relative expression of glutamate receptor 1 flip AMPA receptor subunits in hippocampal astrocytes of epilepsy patients with Ammon's horn sclerosis. | Seifert G et al. | โ | 2004 | โ |
| Evaluation of five diagnostic methods for the detection and quantification of Myxobolus cerebralis. | Kelley GO et al. | โ | 2004 | โ |
| Expression level of valosin-containing protein (p97) is associated with prognosis of esophageal carcinoma. | Yamamoto S et al. | โ | 2004 | โ |
| Four-color multiplex 5' nuclease assay for the simultaneous detection of the factor V Leiden and the prothrombin G20210A mutations. | Ugozzoli LA et al. | โ | 2004 | โ |
| Gene expression profiles in end-stage human idiopathic dilated cardiomyopathy: altered expression of apoptotic and cytoskeletal genes. | Yung CK et al. | โ | 2004 | โ |
| Gene expression profiling in the myelodysplastic syndromes using cDNA microarray technology. | Pellagatti A et al. | โ | 2004 | โ |
| Genomic organization, biology, and diagnosis of Taura syndrome virus and yellowhead virus of penaeid shrimp. | Dhar AK et al. | โ | 2004 | โ |
| High-throughput genotyping of human platelet antigens using the 5'-nuclease assay and minor groove binder probe technology. | Higgins M et al. | โ | 2004 | โ |
| Homogeneous time-resolved fluorescence quenching assay (TruPoint) for nucleic acid detection. | Ylikoski A et al. | โ | 2004 | โ |
| Improved grading of breast adenocarcinomas based on genomic instability. | Kronenwett U et al. | โ | 2004 | โ |
| Inhibition of real-time RT-PCR quantification due to tissue-specific contaminants. | Tichopad A et al. | โ | 2004 | โ |
| Ligation detection reaction-TaqMan procedure for single nucleotide polymorphism detection on genomic DNA. | Borodina TA et al. | โ | 2004 | โ |
| Measurement of relative copy number of CDKN2A/ARF and CDKN2B in bladder cancer by real-time quantitative PCR and multiplex ligation-dependent probe amplification. | Aveyard JS et al. | โ | 2004 | โ |
| Microarray-based AMASE as a novel approach for mutation detection. | Kรคller M et al. | โ | 2004 | โ |
| Multiplex analysis of the most common mutations related to hereditary haemochromatosis: two methods combining specific amplification with capillary electrophoresis. | Gรณmez-Llorente C et al. | โ | 2004 | โ |
| Multiplexed real-time PCR using universal reporters. | Rickert AM et al. | โ | 2004 | โ |
| Multiplex real-time PCR detection of fumonisin-producing and trichothecene-producing groups of Fusarium species. | Bluhm BH et al. | โ | 2004 | โ |
| Mutations of the p53 gene and loss of heterozygosity at chromosome 17p13.1 are associated with increased survivin expression in breast cancer. | Tsuji N et al. | โ | 2004 | โ |
| New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples. | Gueimonde M et al. | โ | 2004 | โ |
| Nucleic acid analysis using an expanded genetic alphabet to quench fluorescence. | Sherrill CB et al. | โ | 2004 | โ |
| p38 Mitogen-Activated protein kinase mediates dual role of ultraviolet B radiation in induction of maturation and apoptosis of monocyte-derived dendritic cells. | Nakagawa S et al. | โ | 2004 | โ |
| Prognostic value of ERM gene expression in human primary breast cancers. | Chotteau-Leliรจvre A et al. | โ | 2004 | โ |
| Quantification of Gram-negative sulphate-reducing bacteria in rice field soil by 16S rRNA gene-targeted real-time PCR. | Stubner S | โ | 2004 | โ |
| Quantification of maternal microchimerism by HLA-specific real-time polymerase chain reaction: studies of healthy women and women with scleroderma. | Lambert NC et al. | โ | 2004 | โ |
| Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR. | Decaro N et al. | โ | 2004 | โ |
| Quantitative analysis of pro-inflammatory cytokine mRNA expression in Theileria annulata-infected cell lines derived from resistant and susceptible cattle. | McGuire K et al. | โ | 2004 | โ |
| Quantitative assessment of disease involvement by follicular lymphoma using real-time polymerase chain reaction measurement of t(14;18)-carrying cells. | Lee WI et al. | โ | 2004 | โ |
| Quantitative detection of Escherichia coli from urine of patients with bacteriuria by real-time PCR. | Hinata N et al. | โ | 2004 | โ |
| Rapid detection of Oenococcus oeni in wine by real-time quantitative PCR. | Pinzani P et al. | โ | 2004 | โ |
| Real-time Fluorescent PCR Techniques to Study Microbial-Host Interactions. | Mackay IM et al. | โ | 2004 | โ |
| Real-time genotyping with oligonucleotide probes containing locked nucleic acids. | Ugozzoli LA et al. | โ | 2004 | โ |
| Real-time nucleic acid-based detection methods for pathogenic bacteria in food. | McKillip JL et al. | โ | 2004 | โ |
| Real-time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters. | Audemard C et al. | โ | 2004 | โ |
| Real-time PCR in the microbiology laboratory. | Mackay IM | โ | 2004 | โ |
| Real-time quantitative assay of telomerase activity using the duplex scorpion primer. | Huang Y et al. | โ | 2004 | โ |
| Real time quantitative PCR as a method to evaluate xenotropic murine leukemia virus removal during pharmaceutical protein purification. | Shi L et al. | โ | 2004 | โ |
| Real-time quantitative PCR-based system for determining transgene copy number in transgenic animals. | Ballester M et al. | โ | 2004 | โ |
| Real-time turbidimetry of LAMP reaction for quantifying template DNA. | Mori Y et al. | โ | 2004 | โ |
| Refinement of single-nucleotide polymorphism genotyping methods on human genomic DNA: amplifluor allele-specific polymerase chain reaction versus ligation detection reaction-TaqMan. | Rickert AM et al. | โ | 2004 | โ |
| Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms. | Petersen K et al. | โ | 2004 | โ |
| Single-cell gene expression analysis: implications for neurodegenerative and neuropsychiatric disorders. | Ginsberg SD et al. | โ | 2004 | โ |
| Single-nucleotide-specific PNA-peptide ligation on synthetic and PCR DNA templates. | Ficht S et al. | โ | 2004 | โ |
| Smallpox and pan-orthopox virus detection by real-time 3'-minor groove binder TaqMan assays on the roche LightCycler and the Cepheid smart Cycler platforms. | Kulesh DA et al. | โ | 2004 | โ |
| Specific inhibition of cyclin-dependent kinase 4/6 by PD 0332991 and associated antitumor activity in human tumor xenografts. | Fry DW et al. | โ | 2004 | โ |
| Stable siRNA-mediated silencing of ATM alters the transcriptional profile of HeLa cells. | Chen S et al. | โ | 2004 | โ |
| Structure of primate and rodent orthologs of the prostate cancer susceptibility gene ELAC2. | Dumont M et al. | โ | 2004 | โ |
| Survivin as a predictor of cis-diamminedichloroplatinum sensitivity in gastric cancer patients. | Nakamura M et al. | โ | 2004 | โ |
| Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells. | Asanuma K et al. | โ | 2004 | โ |
| TaqMan real-time polymerase chain reaction assay for the correlation of Treponema denticola numbers with the severity of periodontal disease. | Yoshida A et al. | โ | 2004 | โ |
| Technology platforms for pharmacogenomic diagnostic assays. | Koch WH | โ | 2004 | โ |
| The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism. | Kushiro T et al. | โ | 2004 | โ |
| The AtPPT1 gene encoding 4-hydroxybenzoate polyprenyl diphosphate transferase in ubiquinone biosynthesis is required for embryo development in Arabidopsis thaliana. | Okada K et al. | โ | 2004 | โ |
| The behavioral and neuroanatomical effects of IB4-saporin treatment in rat models of nociceptive and neuropathic pain. | Tarpley JW et al. | โ | 2004 | โ |
| The candidate oncogene ZNF217 is frequently amplified in colon cancer. | Rooney PH et al. | โ | 2004 | โ |
| The reliable detection of Barley yellow and mild mosaic viruses using real-time PCR (TaqMan). | Mumford R et al. | โ | 2004 | โ |
| The slow skeletal muscle troponin T gene is expressed in developing and diseased human heart. | Barton PJ et al. | โ | 2004 | โ |
| The slow skeletal muscle troponin T gene is expressed in developing and diseased human heart. | Barton PJ et al. | โ | 2004 | โ |
| Tissue-specific gene silencing mediated by a naturally occurring chalcone synthase gene cluster in Glycine max. | Tuteja JH et al. | โ | 2004 | โ |
| Type-specific associations of human papillomavirus load with risk of developing cervical carcinoma in situ. | Moberg M et al. | โ | 2004 | โ |
| Valosin-containing protein (p97) and Ki-67 expression is a useful marker in detecting malignant behavior of pancreatic endocrine neoplasms. | Yamamoto S et al. | โ | 2004 | โ |
| A homogeneous high-throughput genotyping method based on competitive hybridization. | Kiviniemi M et al. | โ | 2003 | โ |
| Angiotensin II receptor blockade prevents microangiopathy and preserves diastolic function in the diabetic rat heart. | Hayashi T et al. | โ | 2003 | โ |
| APC haploinsufficiency, but not CTNNB1 or CDH1 gene mutations, accounts for a fraction of familial adenomatous polyposis patients without APC truncating mutations. | Venesio T et al. | โ | 2003 | โ |
| Application of multivirus spike approach for viral clearance evaluation. | Valera CR et al. | โ | 2003 | โ |
| Brassinolide induces IAA5, IAA19, and DR5, a synthetic auxin response element in Arabidopsis, implying a cross talk point of brassinosteroid and auxin signaling. | Nakamura A et al. | โ | 2003 | โ |
| cDNA-arrays and real-time quantitative PCR techniques in the investigation of chronic Achilles tendinosis. | Alfredson H et al. | โ | 2003 | โ |
| Comparison of HSV-1 thymidine kinase-dependent and -independent inhibition of replication-competent adenoviral vectors by a panel of drugs. | Wildner O et al. | โ | 2003 | โ |
| Detection of Mycobacterium tuberculosis by real-time PCR using pan-mycobacterial primers and a pair of fluorescence resonance energy transfer probes specific for the M. tuberculosis complex. | Drosten C et al. | โ | 2003 | โ |
| Development and validation of a real-time PCR assay for the detection and quantitation of p53 recombinant adenovirus in clinical samples from patients treated with Ad5CMV-p53 (INGN 201). | Saulnier P et al. | โ | 2003 | โ |
| Development of a 5' nuclease-based real-time PCR assay for quantitative detection of cariogenic dental pathogens Streptococcus mutans and Streptococcus sobrinus. | Yoshida A et al. | โ | 2003 | โ |
| Development of Real-Time RT-PCR Assay for Detection of Prunus necrotic ringspot virus in Fruit Trees. | Marbot S et al. | โ | 2003 | โ |
| Evaluation of the performance of LNA and MGB probes in 5'-nuclease PCR assays. | Letertre C et al. | โ | 2003 | โ |
| Expression of the Arp11 gene suppresses the tumorigenicity of PC-14 human lung adenocarcinoma cells. | Shindo-Okada N et al. | โ | 2003 | โ |
| Herpes simplex virus and Meniere's disease. | Vrabec JT | โ | 2003 | โ |
| Human beta-Defensin-2 Induction in Human Foreskin Keratinocyte by Synergetic Stimulation with Foods and Escherichia Coli. | Nishimura E et al. | โ | 2003 | โ |
| Monitoring minimal residual disease in leukemia using real-time quantitative polymerase chain reaction for Wilms tumor gene (WT1). | Tamaki H et al. | โ | 2003 | โ |
| Multiplexed, real-time PCR for quantitative detection of human adenovirus. | Gu Z et al. | โ | 2003 | โ |
| Optimization of a 3'-minor groove binder-DNA probe targeting the uidA gene for rapid identification of Escherichia coli O157:H7 using real-time PCR. | Yoshitomi KJ et al. | โ | 2003 | โ |
| Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR. | Brouwer M et al. | โ | 2003 | โ |
| Quantification of free circulating DNA as a diagnostic marker in lung cancer. | Sozzi G et al. | โ | 2003 | โ |
| [Quantitative determination of CMV-DNA in saliva of patients with bone marrow and stem cell transplantation using TaqMan-PCR]. | Rhinow K et al. | โ | 2003 | โ |
| Quantitative RT-PCR in cirrhotic nodules reveals gene expression changes associated with liver carcinogenesis. | Colombat M et al. | โ | 2003 | โ |
| Real-time PCR based on SYBR-Green I fluorescence: an alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions. | Ponchel F et al. | โ | 2003 | โ |
| Real-time polymerase chain reaction. | Wilhelm J et al. | โ | 2003 | โ |
| Standardized determination of real-time PCR efficiency from a single reaction set-up. | Tichopad A et al. | โ | 2003 | โ |
| The use of hydrolysis and hairpin probes in real-time PCR. | McChlery SM et al. | โ | 2003 | โ |