Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture.
paper
Cited
Public
Unavailable
- Authors
- CΓ‘ceres, A; Banker, G A; Binder, L
- Year
- 1986
- Journal
- The Journal of neuroscience : the official journal of the Society for Neuroscience
- PMID
- 3514816
- DOI
- 10.1523/JNEUROSCI.06-03-00714.1986
- PMCID
- PMC6568475
In dissociated-cell cultures prepared from the embryonic rat hippocampus, neurons establish both axons and dendrites, which differ in geometry, in ultrastructure, and in synaptic polarity. We have used immunocytochemistry with monoclonal antibodies to study the regional distribution of beta-tubulin and micro-tubule-associated protein 2 (MAP2) in hippocampal cultures and their localization during early stages of axonal and dendritic development. After development for a week or more in culture, when axons and dendrites were well-differentiated, the distribution of these two proteins was quite different. Beta-tubulin was present throughout the nerve cell, in soma, dendrites, and axon. It was also present in all classes of non-neuronal cells, astrocytes, fibroblasts, and a presumptive glial progenitor cell. In contrast, MAP2 was preferentially localized to nerve cells; within neurons, MAP2 was present in soma and dendrites, but little or no immunostaining was detectable in axons. Both beta-tubulin and MAP2 were present in nerve cells at the time of plating. From the earliest stages of process extension, beta-tubulin was present in all neuronal processes, both axons and dendrites. Surprisingly, MAP2 was also initially present in both axons and dendrites, extending as far as the axonal growth cone. With subsequent development, MAP2 staining was selectively lost from the axon so that after 1 week in vitro little or no axonal staining remained. Taken together with earlier results (CΓ‘ceres et al., 1984a), these data indicate that the establishment of neuronal polarity, as manifested by the molecular differentiation of the axonal and dendritic cytoskeleton, occurs largely under endogenous control, even under culture conditions in which cell interactions are greatly restricted.(ABSTRACT TRUNCATED AT 250 WORDS)
No figures extracted from this document.
No chunks β full text not yet ingested.
No entities extracted from this document yet.
No uploaded files.
Not in any collection.
No citations found.
In this knowledge base
| Title | Year | PMID |
|---|---|---|
| Alcohol reverses the effects of KCNJ6 (GIRK2) noncoding variants on excitability of human glutamatergic neurons. | 2023 | 36207584 |
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Soluble Ξ²-Amyloid Oligomers Selectively Upregulate TRPC3 in Excitatory Neurons via Calcineurin-Coupled NFAT. | Wang Z et al. | β | 2025 | β |
| Improved immunostaining of nanostructures and cells in human brain specimens through expansion-mediated protein decrowding. | Valdes PA et al. | β | 2024 | β |
| Kinesin Regulation in the Proximal Axon is Essential for Dendrite-selective Transport. | Mendoza CS et al. | β | 2024 | β |
| Magnesium vs. sodium alginate as precursors of calcium alginate: Mechanical differences and advantages in the development of functional neuronal networks. | Della Rosa G et al. | β | 2024 | β |
| TrkB-mediated neuroprotection in female hippocampal neurons is autonomous, estrogen receptor alpha-dependent, and eliminated by testosterone: a proposed model for sex differences in neonatal hippocampal neuronal injury. | Chanana V et al. | β | 2024 | β |
| Alcohol reverses the effects of KCNJ6 (GIRK2) noncoding variants on excitability of human glutamatergic neurons. | Popova D et al. | β | 2023 | β |
| Clathrin-nanoparticles deliver BDNF to hippocampus and enhance neurogenesis, synaptogenesis and cognition in HIV/neuroAIDS mouse model. | Vitaliano GD et al. | β | 2022 | β |
| Establishing neuronal polarity: microtubule regulation during neurite initiation. | Higgs VE et al. | β | 2022 | β |
| Neurons: The Interplay between Cytoskeleton, Ion Channels/Transporters and Mitochondria. | Alberti P et al. | β | 2022 | β |
| Perspectives on Mechanisms Supporting Neuronal Polarity From Small Animals to Humans. | Wilson C et al. | β | 2022 | β |
| Reassessment of Neuronal Tau Distribution in Adult Human Brain and Implications for Tau Pathobiology. | Paterno G et al. | β | 2022 | β |
| The role of the meningeal lymphatic system in local meningeal inflammation and trigeminal nociception. | Mikhailov N et al. | β | 2022 | β |
| Amyloidogenic Processing of Amyloid Precursor Protein Drives Stretch-Induced Disruption of Axonal Transport in hiPSC-Derived Neurons. | Chaves RS et al. | β | 2021 | β |
| A Pictorial History of the Neuronal Cytoskeleton. | Leterrier C | β | 2021 | β |
| Decrowding Expansion Pathology: Unmasking Previously Invisible Nanostructures and Cells in Intact Human Brain Pathology Specimens | Valdes PA et al. | β | 2021 | β |
| Inheritance and flexibility of cell polarity: a clue for understanding human brain development and evolution. | Kalebic N et al. | β | 2021 | β |
| New insights on epigenetic mechanisms supporting axonal development: histone marks and miRNAs. | Wilson C et al. | β | 2021 | β |
| Non-microtubule tubulin-based backbone and subordinate components of postsynaptic density lattices. | Suzuki T et al. | β | 2021 | β |
| Understanding, engineering, and modulating the growth of neural networks: An interdisciplinary approach. | Raj V et al. | β | 2021 | β |
| 3-D multi-electrode arrays detect early spontaneous electrophysiological activity in 3-D neuronal-astrocytic co-cultures. | Vernekar VN et al. | β | 2020 | β |
| Arginase Inhibition Supports Survival and Differentiation of Neuronal Precursors in Adult Alzheimer's Disease Mice. | Polis B et al. | β | 2020 | β |
| Protocol for Evaluating Neuronal Polarity in Murine Models. | Wilson C et al. | β | 2020 | β |
| Reelin Counteracts Chondroitin Sulfate Proteoglycan-Mediated Cortical Dendrite Growth Inhibition. | Zluhan E et al. | β | 2020 | β |
| Targeting chondroitinase ABC to axons enhances the ability of chondroitinase to promote neurite outgrowth and sprouting. | Day P et al. | β | 2020 | β |
| Toward Spontaneous Neuronal Differentiation of SH-SY5Y Cells Using Novel Three-Dimensional Electropolymerized Conductive Scaffolds. | Dominguez-Alfaro A et al. | β | 2020 | β |
| Tuning Neuronal Circuit Formation in 3D Polymeric Scaffolds by Introducing Graphene at the Bio/Material Interface. | Rauti R et al. | β | 2020 | β |
| Chemically Cross-Linked Carbon Nanotube Films Engineered to Control Neuronal Signaling. | BarrejΓ³n M et al. | β | 2019 | β |
| Influence of Nanomolar Deltamethrin on the Hallmarks of Primary Cultured Cortical Neuronal Network and the Role of Ryanodine Receptors. | Zheng J et al. | β | 2019 | β |
| Multifunctionalized hydrogels foster hNSC maturation in 3D cultures and neural regeneration in spinal cord injuries. | Marchini A et al. | β | 2019 | β |
| Somatostatin-Mediated Changes in Microtubule-Associated Proteins and Retinoic Acid-Induced Neurite Outgrowth in SH-SY5Y Cells. | Paik S et al. | β | 2019 | β |
| Acute connexin43 temporal and spatial expression in response to ischemic stroke. | Freitas-Andrade M et al. | β | 2018 | β |
| Effect of sevoflurane on the ATPase activity of hippocampal neurons in a rat model of cerebral ischemia-reperfusion injury via the cAMP-PKA signaling pathway. | Liu TJ et al. | β | 2018 | β |
| Human-Derived Neurons and Neural Progenitor Cells in High Content Imaging Applications. | Harrill JA | β | 2018 | β |
| In Vitro Effects of Chronic Spirolide Treatment on Human Neuronal Stem Cell Differentiation and Cholinergic System Development. | Boente-Juncal A et al. | β | 2018 | β |
| Tauroursodeoxycholic Acid Enhances Mitochondrial Biogenesis, Neural Stem Cell Pool, and Early Neurogenesis in Adult Rats. | Soares R et al. | β | 2018 | β |
| The Development of Neuronal Polarity: A Retrospective View. | Banker G | β | 2018 | β |
| Transdifferentiation of Human Circulating Monocytes Into Neuronal-Like Cells in 20 Days and Without Reprograming. | Bellon A et al. | β | 2018 | β |
| Long-term impact of intrauterine neuroinflammation and treatment with magnesium sulphate and betamethasone: Sex-specific differences in a preterm labor murine model. | Thagard AS et al. | β | 2017 | β |
| Modulation of Synaptic Plasticity by Exercise Training as a Basis for Ischemic Stroke Rehabilitation. | Nie J et al. | β | 2017 | β |
| Stable cell lines of human SH-SY5Y uniformly expressing wild-type or mutant-type FERM domain containing 7 gene. | Pu J et al. | β | 2017 | β |
| Axonal elongation and dendritic branching is enhanced by adenosine A2A receptors activation in cerebral cortical neurons. | Ribeiro FF et al. | β | 2016 | β |
| Effects of 60-GHz millimeter waves on neurite outgrowth in PC12 cells using high-content screening. | Haas AJ et al. | β | 2016 | β |
| Microtubule Dynamics in Neuronal Development, Plasticity, and Neurodegeneration. | Penazzi L et al. | β | 2016 | β |
| The neuritogenic and neuroprotective potential of senegenin against AΞ²-induced neurotoxicity in PC 12 cells. | Jesky R et al. | β | 2016 | β |
| The Subcellular Dynamics of the Gs-Linked Receptor GPR3 Contribute to the Local Activation of PKA in Cerebellar Granular Neurons. | Miyagi T et al. | β | 2016 | β |
| Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth. | De Zio D et al. | β | 2015 | β |
| E2F1 and E2F2 induction in response to DNA damage preserves genomic stability in neuronal cells. | Castillo DS et al. | β | 2015 | β |
| Extracellular and Intracellular Signaling for Neuronal Polarity. | Namba T et al. | β | 2015 | β |
| From 2D to 3D: novel nanostructured scaffolds to investigate signalling in reconstructed neuronal networks. | Bosi S et al. | β | 2015 | β |
| Importance of being Nernst: Synaptic activity and functional relevance in stem cell-derived neurons. | Bradford AB et al. | β | 2015 | β |
| Integrating Image-Based High-Content Screening with Mouse Models Identifies 5-Hydroxydecanoate as a Neuroprotective Drug for Paclitaxel-Induced Neuropathy. | Chen LH et al. | β | 2015 | β |
| Neurological morphofunctional differentiation induced by REAC technology in PC12. A neuro protective model for Parkinson's disease. | Maioli M et al. | β | 2015 | β |
| PKA Inhibitor H89 (N-[2-p-bromocinnamylamino-ethyl]-5-isoquinolinesulfonamide) Attenuates Synaptic Dysfunction and Neuronal Cell Death following Ischemic Injury. | Song J et al. | β | 2015 | β |
| Ultrasoft Alginate Hydrogels Support Long-Term Three-Dimensional Functional Neuronal Networks. | Palazzolo G et al. | β | 2015 | β |
| Intrinsically active and pacemaker neurons in pluripotent stem cell-derived neuronal populations. | Illes S et al. | β | 2014 | β |
| JIP1 mediates anterograde transport of Rab10 cargos during neuronal polarization. | Deng CY et al. | β | 2014 | β |
| Neuronal polarization in vivo: Growing in a complex environment. | Funahashi Y et al. | β | 2014 | β |
| Tropomyosins induce neuritogenesis and determine neurite branching patterns in B35 neuroblastoma cells. | Curthoys NM et al. | β | 2014 | β |
| Accelerated tau pathology with synaptic and neuronal loss in a novel triple transgenic mouse model of Alzheimer's disease. | Saul A et al. | β | 2013 | β |
| Frizzled-5 receptor is involved in neuronal polarity and morphogenesis of hippocampal neurons. | Slater PG et al. | β | 2013 | β |
| Involvement of Ξ±2-antiplasmin in dendritic growth of hippocampal neurons. | Kawashita E et al. | β | 2013 | β |
| Protein phosphatases regulate the growth of developing neurites. | Monroe JD et al. | β | 2013 | β |
| Proteolytically inactive insulin-degrading enzyme inhibits amyloid formation yielding non-neurotoxic aΞ² peptide aggregates. | de Tullio MB et al. | β | 2013 | β |
| Short- and long-term effects of LRRK2 on axon and dendrite growth. | Sepulveda B et al. | β | 2013 | β |
| Simultaneous generation of gradients with gradually changed slope in a microfluidic device for quantifying axon response. | Xiao RR et al. | β | 2013 | β |
| FERM domain containing protein 7 (FRMD7) upregulates the expression of neuronal cytoskeletal proteins and promotes neurite outgrowth in Neuro-2a cells. | Pu J et al. | β | 2012 | β |
| Melatonin increases dendritogenesis in the hilus of hippocampal organotypic cultures. | DomΓnguez-Alonso A et al. | β | 2012 | β |
| Transferability of a modified embryonic stem cell test using a new endpoint for developmental neurotoxicity. | Baek DH et al. | β | 2012 | β |
| Cdk5-mediated phosphorylation of Axin directs axon formation during cerebral cortex development. | Fang WQ et al. | β | 2011 | β |
| Structural model for p75(NTR)-TrkA intracellular domain interaction: a combined FRET and bioinformatics study. | Iacaruso MF et al. | β | 2011 | β |
| Synaptic integration of newly generated neurons in rat dissociated hippocampal cultures. | Cheyne JE et al. | β | 2011 | β |
| While p73 is essential, p63 is completely dispensable for the development of the central nervous system. | Holembowski L et al. | β | 2011 | β |
| Compression alters kinase and phosphatase activity and tau and MAP2 phosphorylation transiently while inducing the fast adaptive dendritic remodeling of underlying cortical neurons. | Chen LJ et al. | β | 2010 | β |
| ENA/VASP downregulation triggers cell death by impairing axonal maintenance in hippocampal neurons. | Franco DL et al. | β | 2010 | β |
| Genetically encoded dendritic marker sheds light on neuronal connectivity in Drosophila. | NicolaΓ― LJ et al. | β | 2010 | β |
| Quantitative assessment of neurite outgrowth in human embryonic stem cell-derived hN2 cells using automated high-content image analysis. | Harrill JA et al. | β | 2010 | β |
| Structure of pleiotrophin- and hepatocyte growth factor-binding sulfated hexasaccharide determined by biochemical and computational approaches. | Li F et al. | β | 2010 | β |
| Trafficking guidance receptors. | Winckler B et al. | β | 2010 | β |
| A mouse model for visualization of GABA(B) receptors. | Casanova E et al. | β | 2009 | β |
| Regulation of mouse embryonic stem cell neural differentiation by retinoic acid. | Kim M et al. | β | 2009 | β |
| Subventricular zone neural progenitors from rapid brain autopsies of elderly subjects with and without neurodegenerative disease. | Leonard BW et al. | β | 2009 | β |
| The neurogenic basic helix-loop-helix transcription factor NeuroD6 concomitantly increases mitochondrial mass and regulates cytoskeletal organization in the early stages of neuronal differentiation. | Kathleen Baxter K et al. | β | 2009 | β |
| Tubulin tyrosination navigates the kinesin-1 motor domain to axons. | Konishi Y et al. | β | 2009 | β |
| Development of an artificial neuronal network with post-mitotic rat fetal hippocampal cells by polyethylenimine. | Liu B et al. | β | 2008 | β |
| HMWMAP2: new perspectives on a pathway to dendritic identity. | Farah CA et al. | β | 2008 | β |
| Model systems for experimental studies: retinal ganglion cells in culture. | Goodyear E et al. | β | 2008 | β |
| Mtap2 is a constituent of the protein network that regulates twik-related K+ channel expression and trafficking. | Sandoz G et al. | β | 2008 | β |
| Plasticity of polarization: changing dendrites into axons in neurons integrated in neuronal circuits. | Gomis-RΓΌth S et al. | β | 2008 | β |
| Abeta1-42 stimulates actin polymerization in hippocampal neurons through Rac1 and Cdc42 Rho GTPases. | Mendoza-Naranjo A et al. | β | 2007 | β |
| Changes in microtubule-associated protein-2 (MAP2) expression during development and after status epilepticus in the immature rat hippocampus. | Jalava NS et al. | β | 2007 | β |
| Induction of axon and dendrite formation during early RGC-5 cell differentiation. | Lieven CJ et al. | β | 2007 | β |
| Neuritogenic activity of chondroitin/dermatan sulfate hybrid chains of embryonic pig brain and their mimicry from shark liver. Involvement of the pleiotrophin and hepatocyte growth factor signaling pathways. | Li F et al. | β | 2007 | β |
| Peroxisome proliferator-activated receptor-alpha is required for the neurotrophic effect of oleic acid in neurons. | Bento-Abreu A et al. | β | 2007 | β |
| Activation of N-methyl-D-aspartate receptor induces a shift of drebrin distribution: disappearance from dendritic spines and appearance in dendritic shafts. | Sekino Y et al. | β | 2006 | β |
| Amelioration of cognitive impairment and changes in microtubule-associated protein 2 after transient global cerebral ischemia are influenced by complex environment experience. | Briones TL et al. | β | 2006 | β |
| Expression of D2 receptor isoforms in cultured neurons reveals equipotent autoreceptor function. | Jomphe C et al. | β | 2006 | β |
| Morphological integration and functional assessment of transplanted neural progenitor cells in healthy and acute ischemic rat eyes. | Grozdanic SD et al. | β | 2006 | β |
| NMDA receptor activation induces translocation and activation of Rac in mouse hippocampal area CA1. | Tejada-Simon MV et al. | β | 2006 | β |
| Time frame of mitral cell development in the mice olfactory bulb. | Blanchart A et al. | β | 2006 | β |
| Differential targeting and functional specialization of sodium channels in cultured cerebellar granule cells. | Osorio N et al. | β | 2005 | β |
| Enhanced neurite outgrowth of rat neural cortical cells on surface-modified films of poly(lactic-co-glycolic acid). | Han DW et al. | β | 2005 | β |
| Expression of the neuronal calcium sensor protein NCS-1 in the developing mouse olfactory pathway. | Treloar HB et al. | β | 2005 | β |
| Genetic analysis of the neuronal and ubiquitous AP-3 adaptor complexes reveals divergent functions in brain. | Seong E et al. | β | 2005 | β |
| PI3K activation by IGF-1 is essential for the regulation of membrane expansion at the nerve growth cone. | Laurino L et al. | β | 2005 | β |
| Stimulation of production of glial cell line-derived neurotrophic factor and nitric oxide by lipopolysaccharide with different dose-responsiveness in cultured rat macrophages. | Hashimoto M et al. | β | 2005 | β |
| Synaptic localization of a functional NADPH oxidase in the mouse hippocampus. | Tejada-Simon MV et al. | β | 2005 | β |
| Acute physiological response of mammalian central neurons to axotomy: ionic regulation and electrical activity. | Mandolesi G et al. | β | 2004 | β |
| The neurotrophic effect of oleic acid includes dendritic differentiation and the expression of the neuronal basic helix-loop-helix transcription factor NeuroD2. | RodrΓguez-RodrΓguez RA et al. | β | 2004 | β |
| 22R-hydroxycholesterol and 9-cis-retinoic acid induce ATP-binding cassette transporter A1 expression and cholesterol efflux in brain cells and decrease amyloid beta secretion. | Koldamova RP et al. | β | 2003 | β |
| Functional synapse formation among rat cortical neurons grown on three-dimensional collagen gels. | O'Shaughnessy TJ et al. | β | 2003 | β |
| Inverse expression of olfactory cell adhesion molecule in a subset of olfactory axons and a subset of mitral/tufted cells in the developing rat main olfactory bulb. | Treloar HB et al. | β | 2003 | β |
| Microtubule-associated protein 2 (MAP2) associates with the NMDA receptor and is spatially redistributed within rat hippocampal neurons after oxygen-glucose deprivation. | Buddle M et al. | β | 2003 | β |
| Morphology of neurons cultured from subdivisions of the mouse cochlear nucleus. | Fitzakerley JL et al. | β | 2003 | β |
| Neurotrophins facilitate synthesis of choline acetyltransferase and tyrosine hydroxylase in cultured mouse neural stem cells independently of their neuronal differentiation. | Ito H et al. | β | 2003 | β |
| Oversulfated dermatan sulfate exhibits neurite outgrowth-promoting activity toward embryonic mouse hippocampal neurons: implications of dermatan sulfate in neuritogenesis in the brain. | Hikino M et al. | β | 2003 | β |
| Subcellular distribution of histamine, GABA and galanin in tuberomamillary neurons in vitro. | Kukko-Lukjanov TK et al. | β | 2003 | β |
| The role of microtubule-associated protein 2c in the reorganization of microtubules and lamellipodia during neurite initiation. | Dehmelt L et al. | β | 2003 | β |
| Alterations to neuronal polarity following permanent axotomy: a quantitative analysis of changes to MAP2a/b and GAP-43 distributions in axotomized motoneurons in the adult cat. | MacDermid V et al. | β | 2002 | β |
| Contact with astroglial membranes induces axonal and dendritic growth of human CNS model neurons and affects the distribution of the growth-associated proteins MAP1B and GAP43. | Piontek J et al. | β | 2002 | β |
| Copper/zinc superoxide dismutase overexpression promotes survival of cortical neurons exposed to neurotoxins in vitro. | Borg J et al. | β | 2002 | β |
| Novel activities of pro-IGF-I E peptides: regulation of morphological differentiation and anchorage-independent growth in human neuroblastoma cells. | Kuo YH et al. | β | 2002 | β |
| Tau function and dysfunction in neurons: its role in neurodegenerative disorders. | Avila J et al. | β | 2002 | β |
| The role of the cytoskeleton in the life cycle of viruses and intracellular bacteria: tracks, motors, and polymerization machines. | Bearer EL et al. | β | 2002 | β |
| A conserved alpha-herpesvirus protein necessary for axonal localization of viral membrane proteins. | Tomishima MJ et al. | β | 2001 | β |
| Administration of FGF-2 to embryonic mouse brain induces hydrocephalic brain morphology and aberrant differentiation of neurons in the postnatal cerebral cortex. | Ohmiya M et al. | β | 2001 | β |
| Agrin differentially regulates the rates of axonal and dendritic elongation in cultured hippocampal neurons. | Mantych KB et al. | β | 2001 | β |
| Calcium-dependent translocation of synaptotagmin to the plasma membrane in the dendrites of developing neurones. | Schwab Y et al. | β | 2001 | β |
| Emergence of axons from distal dendrites of adult mammalian neurons following a permanent axotomy. | Rose PK et al. | β | 2001 | β |
| Evidence for the involvement of Tiam1 in axon formation. | Kunda P et al. | β | 2001 | β |
| Evidence for the role of MAP1B in axon formation. | Gonzalez-Billault C et al. | β | 2001 | β |
| Inhibition of neuronal maturation in primary hippocampal neurons from tau deficient mice. | Dawson HN et al. | β | 2001 | β |
| Microtubule-associated protein 2 phosphorylation is decreased in the human epileptic temporal lobe cortex. | SΓ‘nchez C et al. | β | 2001 | β |
| Plasma membrane ganglioside sialidase regulates axonal growth and regeneration in hippocampal neurons in culture. | Rodriguez JA et al. | β | 2001 | β |
| Sustained phosphorylation of mitogen-activated protein kinase is required for basic fibroblast growth factor-mediated axonal branch formation in cultured rat hippocampal neurons. | Abe K et al. | β | 2001 | β |
| Differential effects of NgCAM and N-cadherin on the development of axons and dendrites by cultured hippocampal neurons. | Esch T et al. | β | 2000 | β |
| Differential neurite growth on astrocyte substrates: interspecies facilitation in green fluorescent protein-transfected rat and human neurons. | van den Pol AN et al. | β | 2000 | β |
| Differentiated neurons retain the capacity to generate axons from dendrites. | Bradke F et al. | β | 2000 | β |
| Drosophila Futsch regulates synaptic microtubule organization and is necessary for synaptic growth. | Roos J et al. | β | 2000 | β |
| Evidence for the involvement of KIF4 in the anterograde transport of L1-containing vesicles. | Peretti D et al. | β | 2000 | β |
| Exogenous BDNF, NT-3 and NT-4 differentially regulate neurite outgrowth in cultured hippocampal neurons. | Labelle C et al. | β | 2000 | β |
| Functional expression of cell surface cannabinoid CB(1) receptors on presynaptic inhibitory terminals in cultured rat hippocampal neurons. | Irving AJ et al. | β | 2000 | β |
| Preferential infection of neuronal and astroglia cells by Akabane virus in primary cultures of fetal bovine brain. | Kitani H et al. | β | 2000 | β |
| Q- and L-type calcium channels control the development of calbindin phenotype in hippocampal pyramidal neurons in vitro. | Boukhaddaoui H et al. | β | 2000 | β |
| Receptor-mediated internalization of somatostatin in rat cortical and hippocampal neurons. | Stroh T et al. | β | 2000 | β |
| Synapse formation proceeds independently of dendritic elongation in cultured hippocampal neurons. | Holgado A et al. | β | 2000 | β |
| The developmental potentials of the caudalmost part of the neural crest are restricted to melanocytes and glia. | Catala M et al. | β | 2000 | β |
| Compartmental organization of the olfactory bulb glomerulus. | Kasowski HJ et al. | β | 1999 | β |
| Gangliosides GM1 and GD1b are not polarized in mature hippocampal neurons. | Shogomori H et al. | β | 1999 | β |
| Glomerular formation in the developing rat olfactory bulb. | Treloar HB et al. | β | 1999 | β |
| Kir 4.1 channel expression in neuroblastomaxglioma hybrid NG108-15 cell line. | Ma W et al. | β | 1999 | β |
| Laminar boundaries persist in the hippocampal dentate molecular layer of the mutant Shaking Rat Kawasaki despite aberrant granule cell migration. | Woodhams PL et al. | β | 1999 | β |
| Recombinant adenovirus is an appropriate vector for endocytotic protein trafficking studies in cultured neurons. | Yuan H et al. | β | 1999 | β |
| Activity-dependent regulation of dendritic BC1 RNA in hippocampal neurons in culture. | Muslimov IA et al. | β | 1998 | β |
| Basic fibroblast growth factor reduces lactic acid-induced neuronal injury in rat hippocampal neurons. | Himmelseher S et al. | β | 1998 | β |
| Central neuronal synapse formation on micropatterned surfaces. | Ma W et al. | β | 1998 | β |
| Changes in dendritic structure and function following hippocampal lesions: correlations with developmental events? | Bulinski JC et al. | β | 1998 | β |
| Developmental regulation and PKC dependence of Alzheimer's-type tau phosphorylations in cultured fetal rat hippocampal neurons. | Combs CK et al. | β | 1998 | β |
| Early synaptogenesis in vitro: role of axon target distance. | van den Pol AN et al. | β | 1998 | β |
| Input summation by cultured pyramidal neurons is linear and position-independent. | Cash S et al. | β | 1998 | β |
| Morphological consequences of altered calcium-dependent transmembrane signaling on the development of cultured cerebellar Purkinje neurons. | Reitstetter R et al. | β | 1998 | β |
| Neuronal expression of the glutamate transporter GLT-1 in hippocampal microcultures. | Mennerick S et al. | β | 1998 | β |
| Rabies virus entry into cultured rat hippocampal neurons. | Lewis P et al. | β | 1998 | β |
| Regulation of hippocampal synaptic plasticity by the tyrosine kinase receptor, REK7/EphA5, and its ligand, AL-1/Ephrin-A5. | Gao WQ et al. | β | 1998 | β |
| Suppression of radixin and moesin alters growth cone morphology, motility, and process formation in primary cultured neurons. | Paglini G et al. | β | 1998 | β |
| Toxicity of cholesterol oxides on cultured neuroretinal cells. | Chang JY et al. | β | 1998 | β |
| Unique responses of differentiating neuronal growth cones to inhibitory cues presented by oligodendrocytes. | Shibata A et al. | β | 1998 | β |
| Cytomechanics of neurite outgrowth from chick brain neurons. | Chada S et al. | β | 1997 | β |
| Delayed localization of synelfin (synuclein, NACP) to presynaptic terminals in cultured rat hippocampal neurons. | Withers GS et al. | β | 1997 | β |
| Differential distribution of stathmin and SCG10 in developing neurons in culture. | Di Paolo G et al. | β | 1997 | β |
| Dlx-2 homeobox gene controls neuronal differentiation in primary cultures of developing basal ganglia. | Ding M et al. | β | 1997 | β |
| Effects of basic fibroblast growth factor on hippocampal neurons after axonal injury. | Himmelseher S et al. | β | 1997 | β |
| Expression and distribution of IGF-1 receptors containing a beta-subunit variant (betagc) in developing neurons. | Mascotti F et al. | β | 1997 | β |
| GABA immunoreactivity in hypothalamic neurons and growth cones in early development in vitro before synapse formation. | van den Pol AN | β | 1997 | β |
| Low density lipoprotein receptor-related protein is expressed early and becomes restricted to a somatodendritic domain during neuronal differentiation in culture. | Brown MD et al. | β | 1997 | β |
| Modulation of neurite branching by protein phosphorylation in cultured rat hippocampal neurons. | Audesirk G et al. | β | 1997 | β |
| Striatal target-induced axonal branching of dopaminergic mesencephalic neurons in culture via diffusible factors. | Manier M et al. | β | 1997 | β |
| Trafficking of cell-surface beta-amyloid precursor protein: evidence that a sorting intermediate participates in synaptic vesicle recycling. | Marquez-Sterling NR et al. | β | 1997 | β |
| A spatial gradient of tau protein phosphorylation in nascent axons. | Mandell JW et al. | β | 1996 | β |
| Characterization and ontogeny of synapse-associated proteins in the developing facial and hypoglossal motor nuclei of the Brazilian opossum. | Swanson JJ et al. | β | 1996 | β |
| Ependymal/subependymal zone cells of postnatal and adult songbird brain generate both neurons and nonneuronal siblings in vitro and in vivo. | Goldman SA et al. | β | 1996 | β |
| Expression and polarization of VGF in developing hippocampal neurons. | Benson DL et al. | β | 1996 | β |
| Expression of glucocorticoid and mineralocorticoid receptors in an immortalized hippocampal neuronal cell line. | Beaman-Hall CM et al. | β | 1996 | β |
| Ganglioside synthesis during the development of neuronal polarity. Major changes occur during axonogenesis and axon elongation, but not during dendrite growth or synaptogenesis. | Hirschberg K et al. | β | 1996 | β |
| Gephyrin accumulates at specific plasmalemma loci during neuronal maturation in vitro. | Colin I et al. | β | 1996 | β |
| Growth cone calcium elevation by GABA. | Obrietan K et al. | β | 1996 | β |
| No diffusion barrier at axon hillock. | Winckler B et al. | β | 1996 | β |
| Polarized expression of GABA transporters in Madin-Darby canine kidney cells and cultured hippocampal neurons. | Ahn J et al. | β | 1996 | β |
| Short exposure to methylazoxymethanol causes a long-term inhibition of axonal outgrowth from cultured embryonic rat hippocampal neurons. | Hoffman JR et al. | β | 1996 | β |
| The effect of basic fibroblast growth factor on glutamate-injured neuroarchitecture and arachidonic acid release in adult hippocampal neurons. | Himmelseher S et al. | β | 1996 | β |
| The effects of ketamine-isomers on neuronal injury and regeneration in rat hippocampal neurons. | Himmelseher S et al. | β | 1996 | β |
| Three distinct neuronal phenotypes exist in embryonic rat hippocampal neurons cultured in basic fibroblast growth factor. | Eubanks JH et al. | β | 1996 | β |
| Translational machinery in dendrites of hippocampal neurons in culture. | Tiedge H et al. | β | 1996 | β |
| An increase in phosphorylation of microtubule-associated protein 2 accompanies dendrite extension during the differentiation of cultured hippocampal neurones. | DΓez-Guerra FJ et al. | β | 1995 | β |
| A regulatory role for sphingolipids in neuronal growth. Inhibition of sphingolipid synthesis and degradation have opposite effects on axonal branching. | Schwarz A et al. | β | 1995 | β |
| Astroglia demonstrate regional differences in their ability to maintain primary dendritic outgrowth from mouse cortical neurons in vitro. | Le Roux PD et al. | β | 1995 | β |
| beta-amyloid fibrils induce tau phosphorylation and loss of microtubule binding. | Busciglio J et al. | β | 1995 | β |
| Cloning of AL-1, a ligand for an Eph-related tyrosine kinase receptor involved in axon bundle formation. | Winslow JW et al. | β | 1995 | β |
| Control of neuronal morphology in vitro: interplay between adhesive substrate forces and molecular instruction. | Lochter A et al. | β | 1995 | β |
| High potassium promotes differentiation of retinal neurons but does not favor rod differentiation. | Araki M et al. | β | 1995 | β |
| Long-term bFGF neuronal culture: reintroduction into serum medium yields neurons and non-neuronal cells with neuronal characteristics. | Eubanks JH et al. | β | 1995 | β |
| Polyamines promote regeneration of injured axons of cultured rat hippocampal neurons. | Chu PJ et al. | β | 1995 | β |
| Regionally specific properties of midbrain glia: I. Interactions with midbrain neurons. | Garcia-Abreu J et al. | β | 1995 | β |
| Selective enhancement of axonal branching of cultured dentate gyrus neurons by neurotrophic factors. | Patel MN et al. | β | 1995 | β |
| Synaptogenesis and distribution of presynaptic axonal varicosities in low density primary cultures of neocortex: an immunocytochemical study utilizing synaptic vesicle-specific antibodies, and an electrophysiological examination utilizing whole cell recording. | Lowenstein PR et al. | β | 1995 | β |
| Transport of dendritic microtubules establishes their nonuniform polarity orientation. | Sharp DJ et al. | β | 1995 | β |
| Accumulation of delta 2-tubulin, a major tubulin variant that cannot be tyrosinated, in neuronal tissues and in stable microtubule assemblies. | Paturle-LafanechΓ¨re L et al. | β | 1994 | β |
| A comparison of the developing dopamine neuron phenotype in cultures of embryonic rat mesencephalon and hypothalamus. | Kapatos G et al. | β | 1994 | β |
| Characterization of basic fibroblast growth factor-mediated acceleration of axonal branching in cultured rat hippocampal neurons. | Aoyagi A et al. | β | 1994 | β |
| Developing rat pineal cells manifest potential of neuronal differentiation in vitro. | Araki M et al. | β | 1994 | β |
| Distribution and trafficking of JHM coronavirus structural proteins and virions in primary neurons and the OBL-21 neuronal cell line. | Pasick JM et al. | β | 1994 | β |
| Diverse distribution and function of fibrous microtubule-associated proteins in the nervous system. | Schoenfeld TA et al. | β | 1994 | β |
| Expression of Bcl-2 protein in murine neural cells in culture. | Mizuguchi M et al. | β | 1994 | β |
| Myelin basic protein mediates extracellular signals that regulate microtubule stability in oligodendrocyte membrane sheets. | Dyer CA et al. | β | 1994 | β |
| Neurotrophin-3 enhances neurite outgrowth in cultured hippocampal pyramidal neurons. | Morfini G et al. | β | 1994 | β |
| Polarized distribution of the trans-Golgi network marker TGN38 during the in vitro development of neocortical neurons: effects of nocodazole and brefeldin A. | Lowenstein PR et al. | β | 1994 | β |
| The axonal gamma-aminobutyric acid transporter GAT-1 is sorted to the apical membranes of polarized epithelial cells. | Pietrini G et al. | β | 1994 | β |
| Effects of selective inhibition of protein kinase C, cyclic AMP-dependent protein kinase, and Ca(2+)-calmodulin-dependent protein kinase on neurite development in cultured rat hippocampal neurons. | Cabell L et al. | β | 1993 | β |
| Even in culture, oligodendrocytes myelinate solely axons. | Lubetzki C et al. | β | 1993 | β |
| Glutamate neurotoxicity in mesencephalic dopaminergic neurons in culture. | Kikuchi S et al. | β | 1993 | β |
| Hippocampal growth cone responses to focally applied electric fields. | Davenport RW et al. | β | 1993 | β |
| Localization of the multiple calmodulin messenger RNAs in differentiated PC12 cells. | Zhang SP et al. | β | 1993 | β |
| NILE/L1 and NCAM-polysialic acid expression on growing axons of isolated neurons. | van den Pol AN et al. | β | 1993 | β |
| Okadaic acid induces early changes in microtubule-associated protein 2 and tau phosphorylation prior to neurodegeneration in cultured cortical neurons. | Arias C et al. | β | 1993 | β |
| Peripherin expression in hippocampal neurons induced by muscle soluble factor(s). | Djabali K et al. | β | 1993 | β |
| Preferential dendritic localization of pericentriolar material in hippocampal pyramidal neurons in culture. | Ferreira A et al. | β | 1993 | β |
| Process formation in Sf9 cells induced by the expression of a microtubule-associated protein 2C-like construct. | LeClerc N et al. | β | 1993 | β |
| The distribution of glutamate receptors in cultured rat hippocampal neurons: postsynaptic clustering of AMPA-selective subunits. | Craig AM et al. | β | 1993 | β |
| The segregation and expression of glutamate receptor subunits in cultured hippocampal neurons. | Eshhar N et al. | β | 1993 | β |
| A real-time analysis of growth cone-target cell interactions during the formation of stable contacts between hippocampal neurons in culture. | Cooper MW et al. | β | 1992 | β |
| Chronic treatment with sabeluzole protects cultured rat brain neurons from the neurotoxic effects of excitatory amino acids. | Pauwels PJ et al. | β | 1992 | β |
| Corticosterone accelerates hypoxia- and cyanide-induced ATP loss in cultured hippocampal astrocytes. | Tombaugh GC et al. | β | 1992 | β |
| Developmental differentiation of MAP2 expression in the central versus the peripheral and efferent projections of the inner ear. | Hafidi A et al. | β | 1992 | β |
| Expression of the class III beta-tubulin isotype in developing neurons in culture. | Ferreira A et al. | β | 1992 | β |
| Glucocorticoids exacerbate hypoxic and hypoglycemic hippocampal injury in vitro: biochemical correlates and a role for astrocytes. | Tombaugh GC et al. | β | 1992 | β |
| Immortal rat hippocampal cell lines exhibit neuronal and glial lineages and neurotrophin gene expression. | Eves EM et al. | β | 1992 | β |
| Isoforms of the Na,K-ATPase are present in both axons and dendrites of hippocampal neurons in culture. | Pietrini G et al. | β | 1992 | β |
| Laminin selectively enhances axonal growth and accelerates the development of polarity by hippocampal neurons in culture. | Lein PJ et al. | β | 1992 | β |
| Redistribution of B-50/growth-associated protein 43 during differentiation and maturation of rat hippocampal neurons in vitro. | van Lookeren Campagne M et al. | β | 1992 | β |
| Alpha 1-antichymotrypsin supports short-term survival of cerebral neurons in culture. | Mizuguchi M et al. | β | 1991 | β |
| Association of microtubule-associated protein (MAP1B) with growing axons in cultured hippocampal neurons. | Fischer I et al. | β | 1991 | β |
| Calpain-mediated proteolysis of microtubule associated proteins MAP1B and MAP2 in developing brain. | Fischer I et al. | β | 1991 | β |
| Evidence for calcium-reducing and excito-protective roles for the calcium-binding protein calbindin-D28k in cultured hippocampal neurons. | Mattson MP et al. | β | 1991 | β |
| Expression of a neuromodulin-beta-galactosidase fusion protein in primary cultured neurons and its accumulation in growth cones. | Liu YC et al. | β | 1991 | β |
| Isolation and characterization of mouse neural precursor cells in primary culture. | Kitani H et al. | β | 1991 | β |
| MAP5 in cultured hippocampal neurons: expression diminishes with time and growth cones are not immunostained. | Denny JB | β | 1991 | β |
| Microtubule dynamics in axons and dendrites. | Baas PW et al. | β | 1991 | β |
| Ontogeny of MAP2 and GFAP antigens in primary cultures of embryonic chick brain. Effect of substratum, oxygen tension, serum and Ara-C. | Bruinink A et al. | β | 1991 | β |
| Synchronous neurite branchings in single goldfish retinal ganglion cells. | Ishida AT et al. | β | 1991 | β |
| Tau protein and the establishment of an axonal morphology. | Kosik KS et al. | β | 1991 | β |
| Temporal pattern of survival and dendritic growth of fetal hippocampal cells transplanted into ischemic lesions of the adult rat hippocampus. | Katayama Y et al. | β | 1991 | β |
| Adhesion and the in vitro development of axons and dendrites. | Prochiantz A et al. | β | 1990 | β |
| Cell culture of cryopreserved human fetal cerebral cortical and hippocampal neurons: neuronal development and responses to trophic factors. | Mattson MP et al. | β | 1990 | β |
| Differential subcellular localization of particular mRNAs in hippocampal neurons in culture. | Kleiman R et al. | β | 1990 | β |
| Localized calcium influx orients axon formation in embryonic hippocampal pyramidal neurons. | Mattson MP et al. | β | 1990 | β |
| Nerve growth factor potentiates the neurotoxicity of beta amyloid. | Yankner BA et al. | β | 1990 | β |
| Neurotrophic and neurotoxic effects of amyloid beta protein: reversal by tachykinin neuropeptides. | Yankner BA et al. | β | 1990 | β |
| Phosphorylation of microtubule proteins in rat brain at different developmental stages: comparison with that found in neuronal cultures. | DΓaz-Nido J et al. | β | 1990 | β |
| Polarized sorting of viral glycoproteins to the axon and dendrites of hippocampal neurons in culture. | Dotti CG et al. | β | 1990 | β |
| An immunohistochemical characterization of the primitive and maturing neuroepithelial components in the OTT-6050 transplantable mouse teratoma. | Caccamo DV et al. | β | 1989 | β |
| An immunohistochemical study of neuropeptides and neuronal cytoskeletal proteins in the neuroepithelial component of a spontaneous murine ovarian teratoma. Primitive neuroepithelium displays immunoreactivity for neuropeptides and neuron-associated beta-tubulin isotype. | Caccamo DV et al. | β | 1989 | β |
| Characteristics of microtubules at the different stages of neuronal differentiation and maturation. | Meininger V et al. | β | 1989 | β |
| Compartmentation of alpha-tubulin in neurons: identification of a somatodendritic-specific variant of alpha-tubulin. | Baas PW et al. | β | 1989 | β |
| Development and selective neurodegeneration in cell cultures from different hippocampal regions. | Mattson MP et al. | β | 1989 | β |
| Excitatory and inhibitory neurotransmitters in the generation and degeneration of hippocampal neuroarchitecture. | Mattson MP et al. | β | 1989 | β |
| Experimental observations on the development of polarity by hippocampal neurons in culture. | Goslin K et al. | β | 1989 | β |
| Immunocytochemical localization of microtubule-associated proteins 1A and 2 in the rat retina. | Okabe S et al. | β | 1989 | β |
| Influence of extracellular matrix proteins on the expression of neuronal polarity. | Chamak B et al. | β | 1989 | β |
| Localization and mobility of omega-conotoxin-sensitive Ca2+ channels in hippocampal CA1 neurons. | Jones OT et al. | β | 1989 | β |
| Microtubular reorganization and dendritic growth response in Alzheimer's disease. | McKee AC et al. | β | 1989 | β |
| Microtubule formation and neurite growth in cerebellar macroneurons which develop in vitro: evidence for the involvement of the microtubule-associated proteins, MAP-1a, HMW-MAP2 and Tau. | Ferreira A et al. | β | 1989 | β |
| Monoclonal antibody markers for amphibian oligodendrocytes and neurons. | Steen P et al. | β | 1989 | β |
| Neuroblastic differentiation potential of the human retinoblastoma cell lines Y-79 and WERI-Rb1 maintained in an organ culture system. An immunohistochemical, electron microscopic, and biochemical study. | Herman MM et al. | β | 1989 | β |
| Rapid turnover of microtubule-associated protein MAP2 in the axon revealed by microinjection of biotinylated MAP2 into cultured neurons. | Okabe S et al. | β | 1989 | β |
| Rearrangement of microtubule associated protein parallels the morphological transformation of neurons from dorsal root ganglion. | HernΓ‘ndez MA et al. | β | 1989 | β |
| Survival and growth of hippocampal neurons in defined medium at low density: advantages of a sandwich culture technique or low oxygen. | Brewer GJ et al. | β | 1989 | β |
| The establishment of polarity by hippocampal neurons: the relationship between the stage of a cell's development in situ and its subsequent development in culture. | Fletcher TL et al. | β | 1989 | β |
| The expression of acetylated microtubules during axonal and dendritic growth in cerebellar macroneurons which develop in vitro. | Ferreira A et al. | β | 1989 | β |
| Tyrosination state of tubulin and the activity of tubulin:tyrosine ligase and tubulin carboxypeptidase in the developing retina of the chick. | Arregui C et al. | β | 1989 | β |
| What distinguishes axons from dendrites? Neurons know more than we do. | Sargent PB | β | 1989 | β |
| Development of neuronal polarity: GAP-43 distinguishes axonal from dendritic growth cones. | Goslin K et al. | β | 1988 | β |
| Interactions between entorhinal axons and target hippocampal neurons: a role for glutamate in the development of hippocampal circuitry. | Mattson MP et al. | β | 1988 | β |
| Intracellular messengers in the generation and degeneration of hippocampal neuroarchitecture. | Mattson MP et al. | β | 1988 | β |
| Isolated hippocampal neurons in cryopreserved long-term cultures: development of neuroarchitecture and sensitivity to NMDA. | Mattson MP et al. | β | 1988 | β |
| Microtubule-associated protein 2 (MAP 2) immunoreactivity in human fetal neocortex. | Sims KB et al. | β | 1988 | β |
| Microtubule-associated proteins characteristic of embryonic brain are found in the adult mammalian retina. | Tucker RP et al. | β | 1988 | β |
| Morphological differentiation of embryonic rat sympathetic neurons in tissue culture. I. Conditions under which neurons form axons but not dendrites. | Bruckenstein DA et al. | β | 1988 | β |
| Neurotransmitters in the regulation of neuronal cytoarchitecture. | Mattson MP | β | 1988 | β |
| Polarity orientation of microtubules in hippocampal neurons: uniformity in the axon and nonuniformity in the dendrite. | Baas PW et al. | β | 1988 | β |
| Protein synthesis and processing in cytoplasmic microdomains beneath postsynaptic sites on CNS neurons. A mechanism for establishing and maintaining a mosaic postsynaptic receptive surface. | Steward O et al. | β | 1988 | β |
| The distribution of microtubule-associated protein 2 changes when dendritic growth is induced in rat sympathetic neurons in vitro. | Higgins D et al. | β | 1988 | β |
| Aluminum and Alzheimer's disease: perspectives for a cytoskeletal mechanism. | Bertholf RL | β | 1987 | β |
| An immunocytochemical analysis of the ontogeny of the microtubule-associated proteins MAP-2 and Tau in the nervous system of the rat. | Ferreira A et al. | β | 1987 | β |
| Changes in the number of chick ciliary ganglion neuron processes with time in cell culture. | Role LW et al. | β | 1987 | β |
| Role of microtubules in the cytoplasmic compartmentation of neurons. | Baas PW et al. | β | 1987 | β |
| Sequential appearance of cytoskeletal components during the early stages of neurite outgrowth from cerebellar granule cells in vitro. | Cambray-Deakin MA et al. | β | 1987 | β |
| The expression and distribution of the microtubule-associated proteins tau and microtubule-associated protein 2 in hippocampal neurons in the rat in situ and in cell culture. | Dotti CG et al. | β | 1987 | β |