The involvement of AMP-activated protein kinases in the anti-inflammatory effect of nicotine in vivo and in vitro.
- Authors
- Cheng, Pao-Yun; Lee, Yen-Mei; Law, Kwok-Keung; Lin, Chia-Wei; Yen, Mao-Hsiung
- Year
- 2007
- Journal
- Biochemical pharmacology
- PMID
- 17869227
- DOI
- 10.1016/j.bcp.2007.08.004
AMP-activated protein kinase (AMPK) is the downstream component of a kinase cascade that plays a pivotal role in energy homeostasis. AMPK has recently emerged as an attractive and novel target for inflammatory disorders. Thus, the aim of this study was to assess the role of AMPKalpha in the anti-inflammatory effect of nicotine in carrageenan-induced rat paw edema model and to evaluate the mechanism of nicotine-induced AMPKalpha phosphorylation in RAW 264.7 cells. The results indicate that nicotine alleviated paw edema and the activation of AMPKalpha involved in the anti-inflammatory effect of nicotine in vivo. In addition, nicotine was able to activate AMPKalpha phosphorylation in macrophages and this effect was mediated through nicotinic acetylcholine receptors. Furthermore, nicotine significantly induced the phosphorylation of Akt and the Ca(2+)/calmodulin-dependent protein kinase kinase (CaMKK) protein expression in macrophages. Wortmannin, a specific inhibitor of phosphotidylinositol 3-kinase (PI3K), suppressed nicotine-induced Akt and AMPKalpha phosphorylation. STO-609, a CaMKK inhibitor, not only inhibited the activation of AMPKalpha but also suppressed the phosphorylation of Akt induced by nicotine. In conclusion, both of CaMKK and PI3K/Akt pathways are involved in the nicotine-induced AMPKalpha phosphorylation in macrophages, and the interaction of CaMKK and Akt may exist. AMPKalpha is a novel and critical component of anti-inflammatory effect of nicotine.
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| 5'AMP-activated protein kinase: an emerging target of phytochemicals to treat chronic inflammatory diseases. | Abdul Khaliq H et al. | β | 2024 | β |
| Nicotine's effect on cognition, a friend or foe? | Wang Q et al. | β | 2023 | β |
| Acute Nicotine Treatment Alleviates LPS-Induced Impairment of Fear Memory Reconsolidation Through AMPK Activation and CRTC1 Upregulation in Hippocampus. | Shu H et al. | β | 2020 | β |
| Maternal high fat diet consumption reduces liver alpha7 nicotinic cholinergic receptor expression and impairs insulin signalling in the offspring. | Costa SO et al. | β | 2020 | β |
| Nicotine Attenuates Osteoarthritis Pain and Matrix Metalloproteinase-9 Expression via the Ξ±7 Nicotinic Acetylcholine Receptor. | Teng P et al. | β | 2019 | β |
| Pharmacological Effects and Regulatory Mechanisms of Tobacco Smoking Effects on Food Intake and Weight Control. | Hu T et al. | β | 2018 | β |
| Inhibitory effects of nicotine derived from cigarette smoke on thymic stromal lymphopoietin production in epidermal keratinocytes. | Dong J et al. | β | 2016 | β |
| Suppression of abdominal aortic aneurysm formation by AR-R17779, an agonist for the Ξ±7 nicotinic acetylcholine receptor. | Watanabe A et al. | β | 2016 | β |
| Genome-wide association study of nicotine dependence in American populations: identification of novel risk loci in both African-Americans and European-Americans. | Gelernter J et al. | β | 2015 | β |
| Regulation of Cigarette Smoke Induction of IL-8 in Macrophages by AMP-activated Protein Kinase Signaling. | Ko HK et al. | β | 2015 | β |
| The role of alpha-7 nicotinic receptors in food intake behaviors. | McFadden KL et al. | β | 2014 | β |
| Identification and characterization of poly(I:C)-induced molecular responses attenuated by nicotine in mouse macrophages. | Cui WY et al. | β | 2013 | β |
| Overexpression of MDR1 and survivin, and decreased Bim expression mediate multidrug-resistance in multiple myeloma cells. | Tsubaki M et al. | β | 2012 | β |
| Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance. | Wang X et al. | β | 2011 | β |
| Nonsteroidal anti-inflammatory drug flufenamic acid is a potent activator of AMP-activated protein kinase. | Chi Y et al. | β | 2011 | β |
| Reciprocal effects of Ξ±-lipoic acid on adenosine monophosphate-activated protein kinase activity in obesity induced by ovariectomy in rats. | Cheng PY et al. | β | 2011 | β |
| The role of hypothalamic AMP-activated protein kinase in ovariectomy-induced obesity in rats. | Tsai YC et al. | β | 2010 | β |
| Activation and signaling by the AMP-activated protein kinase in endothelial cells. | Fisslthaler B et al. | β | 2009 | β |
| AMP-activated protein kinase mediates the interferon-gamma-induced decrease in intestinal epithelial barrier function. | Scharl M et al. | β | 2009 | β |
| Elevated corticosterone associated with food deprivation upregulates expression in rat skeletal muscle of the mTORC1 repressor, REDD1. | McGhee NK et al. | β | 2009 | β |
| Flow activation of AMP-activated protein kinase in vascular endothelium leads to KrΓΌppel-like factor 2 expression. | Young A et al. | β | 2009 | β |
| The vagus nerve as a modulator of intestinal inflammation. | Van Der Zanden EP et al. | β | 2009 | β |
| Targeting AMPK: a new therapeutic opportunity in breast cancer. | Hadad SM et al. | β | 2008 | β |