Methods for making induced pluripotent stem cells: reprogramming à la carte.
- Authors
- González, Federico; Boué, Stéphanie; Izpisúa Belmonte, Juan Carlos
- Year
- 2011
- Journal
- Nature reviews. Genetics
- PMID
- 21339765
- DOI
- 10.1038/nrg2937
Pluripotent stem-cell lines can be obtained through the reprogramming of somatic cells from different tissues and species by ectopic expression of defined factors. In theory, these cells--known as induced pluripotent stem cells (iPSCs)--are suitable for various purposes, including disease modelling, autologous cell therapy, drug or toxicity screening and basic research. Recent methodological improvements are increasing the ease and efficiency of reprogramming, and reducing the genomic modifications required to complete the process. However, depending on the downstream applications, certain technologies have advantages over others. Here, we provide a comprehensive overview of the existing reprogramming approaches with the aim of providing readers with a better understanding of the reprogramming process and a basis for selecting the most suitable method for basic or clinical applications.
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In this knowledge base
| Title | Year | PMID |
|---|---|---|
| Using human stem cells as a model system to understand the neural mechanisms of alcohol use disorders: Current status and outlook. | 2019 | 30087005 |
External
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| Human pluripotent stem cell based islet models for diabetes research. | Balboa D et al. | — | 2015 | → |
| Improved approach for chondrogenic differentiation of human induced pluripotent stem cells. | Nejadnik H et al. | — | 2015 | → |
| Induced pluripotent stem cell technology for modelling and therapy of cerebellar ataxia. | Watson LM et al. | — | 2015 | → |
| Minicircle DNA vectors for gene therapy: advances and applications. | Gaspar V et al. | — | 2015 | → |
| Modeling the epigenetic attractors landscape: toward a post-genomic mechanistic understanding of development. | Davila-Velderrain J et al. | — | 2015 | → |
| Modelling sarcomeric cardiomyopathies in the dish: from human heart samples to iPSC cardiomyocytes. | Eschenhagen T et al. | — | 2015 | → |
| Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. | Diecke S et al. | — | 2015 | → |
| Perspectives of induced pluripotent stem cells for cardiovascular system regeneration. | Csöbönyeiová M et al. | — | 2015 | → |
| Prmt5 is required for germ cell survival during spermatogenesis in mice. | Wang Y et al. | — | 2015 | → |
| Prolonged proteasome inhibition cyclically upregulates Oct3/4 and Nanog gene expression, but reduces induced pluripotent stem cell colony formation. | Floyd ZE et al. | — | 2015 | → |
| Protein arginine methyltransferase 5 (Prmt5) is required for germ cell survival during mouse embryonic development. | Wang Y et al. | — | 2015 | → |
| Reprogramming barriers and enhancers: strategies to enhance the efficiency and kinetics of induced pluripotency. | Ebrahimi B | — | 2015 | → |
| Restoration of Physiologically Responsive Low-Density Lipoprotein Receptor-Mediated Endocytosis in Genetically Deficient Induced Pluripotent Stem Cells. | Ramakrishnan VM et al. | — | 2015 | → |
| The unforeseen challenge: from genotype-to-phenotype in cell populations. | Braun E | — | 2015 | → |
| An integration-defective lentivirus-based resource for site-specific targeting of an edited safe-harbour locus in the human genome. | Torres R et al. | — | 2014 | → |
| Application of stems cells in wound healing--an update. | Teng M et al. | — | 2014 | → |
| CD44 is a negative cell surface marker for pluripotent stem cell identification during human fibroblast reprogramming. | Quintanilla RH et al. | — | 2014 | → |
| Critical lysine residues of Klf4 required for protein stabilization and degradation. | Lim KH et al. | — | 2014 | → |
| Dedifferentiation and reprogramming: origins of cancer stem cells. | Friedmann-Morvinski D et al. | — | 2014 | → |
| Derivation of transgene-free human induced pluripotent stem cells from human peripheral T cells in defined culture conditions. | Kishino Y et al. | — | 2014 | → |
| Epigenetic landscapes explain partially reprogrammed cells and identify key reprogramming genes. | Lang AH et al. | — | 2014 | → |
| Epigenetic state network approach for describing cell phenotypic transitions. | Wang P et al. | — | 2014 | → |
| Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells. | Kadari A et al. | — | 2014 | → |
| Generation and characterization of functional cardiomyocytes derived from human T cell-derived induced pluripotent stem cells. | Seki T et al. | — | 2014 | → |
| Generation of induced pluripotent stem cells from virus-free in vivo reprogramming of BALB/c mouse liver cells. | de Lázaro I et al. | — | 2014 | → |
| Generation of mouse induced pluripotent stem cells by protein transduction. | Nemes C et al. | — | 2014 | → |
| Generation of thyroid follicular cells from pluripotent stem cells: potential for regenerative medicine. | Sewell W et al. | — | 2014 | → |
| Induced pluripotent stem cell-derived cardiomyocytes for drug development and toxicity testing. | Sinnecker D et al. | — | 2014 | → |
| Induced pluripotent stem (iPS) cells: a new source for cell-based therapeutics? | de Lázaro I et al. | — | 2014 | → |
| MicroRNAs: modulators of cell identity, and their applications in tissue engineering. | Ribeiro AO et al. | — | 2014 | → |
| Natural killer cells for cancer immunotherapy: pluripotent stem cells-derived NK cells as an immunotherapeutic perspective. | Eguizabal C et al. | — | 2014 | → |
| Nonviral delivery for reprogramming to pluripotency and differentiation. | Park HJ et al. | — | 2014 | → |
| OCT4: dynamic DNA binding pioneers stem cell pluripotency. | Jerabek S et al. | — | 2014 | → |
| ONSL and OSKM cocktails act synergistically in reprogramming human somatic cells into induced pluripotent stem cells. | Jung L et al. | — | 2014 | → |
| Optimized Generation of Functional Neutrophils and Macrophages from Patient-Specific Induced Pluripotent Stem Cells: Ex Vivo Models of X(0)-Linked, AR22(0)- and AR47(0)- Chronic Granulomatous Diseases. | Brault J et al. | — | 2014 | → |
| PRMT5 is required for human embryonic stem cell proliferation but not pluripotency. | Gkountela S et al. | — | 2014 | → |
| Recent technological updates and clinical applications of induced pluripotent stem cells. | Diecke S et al. | — | 2014 | → |
| Research highlights: Microtechnologies for engineering the cellular environment. | Tseng P et al. | — | 2014 | → |
| Second generation codon optimized minicircle (CoMiC) for nonviral reprogramming of human adult fibroblasts. | Diecke S et al. | — | 2014 | → |
| Stem cells: are we ready for therapy? | Schroeder IS | — | 2014 | → |
| Stem cells: potential and challenges for kidney repair. | Herrera M et al. | — | 2014 | → |
| The aging signature: a hallmark of induced pluripotent stem cells? | Rohani L et al. | — | 2014 | → |
| A comparison of pluripotency and differentiation status of four mesenchymal adult stem cells. | Adegani FJ et al. | — | 2013 | → |
| Ancestral gene and "complementary" antibody dominate early ontogeny. | Arend P | — | 2013 | → |
| Characterization of the early events leading to totipotency in an Arabidopsis protoplast liquid culture by temporal transcript profiling. | Chupeau MC et al. | — | 2013 | → |
| Combining small molecules for cell reprogramming through an interatomic analysis. | Feltes BC et al. | — | 2013 | → |
| Concealing cellular defects in pluripotent stem cells. | Zhang W et al. | — | 2013 | → |
| Concise review: carbon nanotechnology: perspectives in stem cell research. | Pryzhkova MV | — | 2013 | → |
| Concise review: the dynamics of induced pluripotency and its behavior captured in gene network motifs. | Muraro MJ et al. | — | 2013 | → |
| Design and generation of recombinant rabies virus vectors. | Osakada F et al. | — | 2013 | → |
| Development of a new approach to aid in visual identification of murine iPS colonies using a fuzzy logic decision support system. | Bassaneze V et al. | — | 2013 | → |
| Disease-corrected hepatocyte-like cells from familial hypercholesterolemia-induced pluripotent stem cells. | Fattahi F et al. | — | 2013 | → |
| Does the adult stroma contain stem cells? | Schäfer R | — | 2013 | → |
| Efficient generation of human iPSCs by a synthetic self-replicative RNA. | Yoshioka N et al. | — | 2013 | → |
| Efficient production of retroviruses using PLGA/bPEI-DNA nanoparticles and application for reprogramming somatic cells. | Seo EJ et al. | — | 2013 | → |
| Engineered MSCs from Patient-Specific iPS Cells. | Eberle I et al. | — | 2013 | → |
| Generating insulin-producing cells for diabetic therapy: existing strategies and new development. | Shen J et al. | — | 2013 | → |
| Generation and characterization of transgene-free human induced pluripotent stem cells and conversion to putative clinical-grade status. | Awe JP et al. | — | 2013 | → |
| Generation of induced pluripotent stem cells from human foetal fibroblasts using the Sleeping Beauty transposon gene delivery system. | Davis RP et al. | — | 2013 | → |
| Gene regulatory networks mediating canonical Wnt signal-directed control of pluripotency and differentiation in embryo stem cells. | Zhang X et al. | — | 2013 | → |
| Global transcriptional analysis of nuclear reprogramming in the transition from MEFs to iPSCs. | Dong F et al. | — | 2013 | → |
| Hematopoietic specification from human pluripotent stem cells: current advances and challenges toward de novo generation of hematopoietic stem cells. | Slukvin II | — | 2013 | → |
| Homologous recombination DNA repair genes play a critical role in reprogramming to a pluripotent state. | González F et al. | — | 2013 | → |
| Human induced pluripotent stem cells as a tool to model a form of Leber congenital amaurosis. | Lustremant C et al. | — | 2013 | → |
| Human kidney cell reprogramming: applications for disease modeling and personalized medicine. | O'Neill AC et al. | — | 2013 | → |
| In-a-dish: induced pluripotent stem cells as a novel model for human diseases. | Beltrão-Braga PC et al. | — | 2013 | → |
| Induced pluripotency and direct reprogramming: a new window for treatment of neurodegenerative diseases. | Li R et al. | — | 2013 | → |
| Induced pluripotent stem cell reprogramming by integration-free Sendai virus vectors from peripheral blood of patients with craniometaphyseal dysplasia. | Chen IP et al. | — | 2013 | → |
| Induced Pluripotent Stem Cells and Disorders of the Nervous System: Progress, Problems, and Prospects. | Faiz M et al. | — | 2013 | → |
| In vivo cell reprogramming towards pluripotency by virus-free overexpression of defined factors. | Yilmazer A et al. | — | 2013 | → |
| In vivo reprogramming of adult somatic cells to pluripotency by overexpression of Yamanaka factors. | Yilmazer A et al. | — | 2013 | → |
| Is aging a barrier to reprogramming? Lessons from induced pluripotent stem cells. | Phanthong P et al. | — | 2013 | → |
| Isogenic human pluripotent stem cell pairs reveal the role of a KCNH2 mutation in long-QT syndrome. | Bellin M et al. | — | 2013 | → |
| Learning the molecular mechanisms of the reprogramming factors: let's start from microRNAs. | Yang CS et al. | — | 2013 | → |
| New opportunities: harnessing induced pluripotency for discovery in diabetes and metabolism. | Teo AK et al. | — | 2013 | → |
| Pluripotent stem cell models of human heart disease. | Moretti A et al. | — | 2013 | → |
| Progress in the reprogramming of somatic cells. | Ma T et al. | — | 2013 | → |
| Review of transplantation of neural stem/progenitor cells for spinal cord injury. | Mothe AJ et al. | — | 2013 | → |
| RNA-based tools for nuclear reprogramming and lineage-conversion: towards clinical applications. | Bernal JA | — | 2013 | → |
| Sleeping Beauty transposon-based system for cellular reprogramming and targeted gene insertion in induced pluripotent stem cells. | Grabundzija I et al. | — | 2013 | → |
| Stress cycles in stem cells/iPSCs development: implications for tissue repair. | Grafi G | — | 2013 | → |
| Technological overview of iPS induction from human adult somatic cells. | Bayart E et al. | — | 2013 | → |
| Therapeutic potential of human-induced pluripotent stem cell-derived endothelial cells in a bleomycin-induced scleroderma mouse model. | Azhdari M et al. | — | 2013 | → |
| Therapeutic potential of human induced pluripotent stem cell-derived mesenchymal stem cells in mice with lethal fulminant hepatic failure. | Moslem M et al. | — | 2013 | → |
| The ROSA26-iPSC mouse: a conditional, inducible, and exchangeable resource for studying cellular (De)differentiation. | Haenebalcke L et al. | — | 2013 | → |
| To clone or not to clone? Induced pluripotent stem cells can be generated in bulk culture. | Willmann CA et al. | — | 2013 | → |
| Urine as a source of stem cells. | Benda C et al. | — | 2013 | → |
| Will brain cells derived from induced pluripotent stem cells or directly converted from somatic cells (iNs) be useful for schizophrenia research? | Filippich C et al. | — | 2013 | → |
| Zinc finger nuclease-expressing baculoviral vectors mediate targeted genome integration of reprogramming factor genes to facilitate the generation of human induced pluripotent stem cells. | Phang RZ et al. | — | 2013 | → |
| Zscan4 promotes genomic stability during reprogramming and dramatically improves the quality of iPS cells as demonstrated by tetraploid complementation. | Jiang J et al. | — | 2013 | → |
| Advances and applications of induced pluripotent stem cells. | Pietronave S et al. | — | 2012 | → |
| Advances in stem cell therapy for spinal cord injury. | Mothe AJ et al. | — | 2012 | → |
| A new efficient protocol for directed differentiation of retinal pigmented epithelial cells from normal and retinal disease induced pluripotent stem cells. | Zahabi A et al. | — | 2012 | → |
| An update review of stem cell applications in burns and wound care. | Huang L et al. | — | 2012 | → |
| Concise review: Cord blood banking, transplantation and induced pluripotent stem cell: success and opportunities. | Rao M et al. | — | 2012 | → |
| Concise review: Embryonic stem cells versus induced pluripotent stem cells: the game is on. | Puri MC et al. | — | 2012 | → |
| Concise review: Induced pluripotent stem cell-derived mesenchymal stem cells: progress toward safe clinical products. | Jung Y et al. | — | 2012 | → |
| Defective antiviral responses of induced pluripotent stem cells to baculoviral vector transduction. | Chen GY et al. | — | 2012 | → |
| Delivering factors for reprogramming a somatic cell to pluripotency. | Um SH | — | 2012 | → |
| Development of Sendai virus vectors and their potential applications in gene therapy and regenerative medicine. | Nakanishi M et al. | — | 2012 | → |
| Efficient iPS cell production with the MyoD transactivation domain in serum-free culture. | Hirai H et al. | — | 2012 | → |
| Enhancers: emerging roles in cell fate specification. | Ong CT et al. | — | 2012 | → |
| From understanding the development landscape of the canonical fate-switch pair to constructing a dynamic landscape for two-step neural differentiation. | Qiu X et al. | — | 2012 | → |
| Fully functional hair follicle regeneration through the rearrangement of stem cells and their niches. | Toyoshima KE et al. | — | 2012 | → |
| Generation and applications of human pluripotent stem cells induced into neural lineages and neural tissues. | Martinez Y et al. | — | 2012 | → |
| Human pluripotent stem cells for modeling toxicity. | Sison-Young RL et al. | — | 2012 | → |
| Identification of Oct4-activating compounds that enhance reprogramming efficiency. | Li W et al. | — | 2012 | → |
| Induced pluripotent stem cell modeling of complex genetic diseases. | Hinson JT et al. | — | 2012 | → |
| Induced pluripotent stem cells: the new patient? | Bellin M et al. | — | 2012 | → |
| Inducing pluripotency using in vivo gene therapy. | Gardlik R | — | 2012 | → |
| Inhibition of glycogen synthase kinase-3 promotes efficient derivation of pluripotent stem cells from neonatal mouse testis. | Moraveji SF et al. | — | 2012 | → |
| Lineage conversion methodologies meet the reprogramming toolbox. | Sancho-Martinez I et al. | — | 2012 | → |
| miRNAs in ESC differentiation. | Berardi E et al. | — | 2012 | → |
| Neurovascular pathophysiology in cerebral ischemia, dementia and the ageing brain - current trends in basic, translational and clinical research. | Boltze J et al. | — | 2012 | → |
| Novel live alkaline phosphatase substrate for identification of pluripotent stem cells. | Singh U et al. | — | 2012 | → |
| Progress made in the reprogramming field: new factors, new strategies and a new outlook. | Hussein SM et al. | — | 2012 | → |
| Small molecules, big roles -- the chemical manipulation of stem cell fate and somatic cell reprogramming. | Zhang Y et al. | — | 2012 | → |
| Standardization of pluripotent stem cell cultures for toxicity testing. | Pistollato F et al. | — | 2012 | → |
| The epigenome in early vertebrate development. | Bogdanović O et al. | — | 2012 | → |
| The gene expression profiles of induced pluripotent stem cells (iPSCs) generated by a non-integrating method are more similar to embryonic stem cells than those of iPSCs generated by an integrating method. | Liu Y et al. | — | 2012 | → |
| The roles of the reprogramming factors Oct4, Sox2 and Klf4 in resetting the somatic cell epigenome during induced pluripotent stem cell generation. | Schmidt R et al. | — | 2012 | → |
| The stability of the induced epigenetic programs. | Barrero MJ | — | 2012 | → |
| Time to reconsider stem cell induction strategies. | Denker HW | — | 2012 | → |
| Using human pluripotent stem cells to study post-transcriptional mechanisms of neurodegenerative diseases. | Patani R et al. | — | 2012 | → |
| Vascular differentiation from embryonic stem cells: novel technologies and therapeutic promises. | Descamps B et al. | — | 2012 | → |
| Zscan4 transiently reactivates early embryonic genes during the generation of induced pluripotent stem cells. | Hirata T et al. | — | 2012 | → |
| Assessment of research models for testing gene-environment interactions. | Inselman AL et al. | — | 2011 | → |
| Bmi1 puSHHes reprogramming. | Li H et al. | — | 2011 | → |
| Capturing Alzheimer's disease genomes with induced pluripotent stem cells: prospects and challenges. | Israel MA et al. | — | 2011 | → |
| Cell-based therapeutics for liver disorders. | Vosough M et al. | — | 2011 | → |
| Concise review: alchemy of biology: generating desired cell types from abundant and accessible cells. | Pournasr B et al. | — | 2011 | → |
| Constructing and deconstructing stem cell models of neurological disease. | Han SS et al. | — | 2011 | → |
| Diseases in a dish: modeling human genetic disorders using induced pluripotent cells. | Tiscornia G et al. | — | 2011 | → |
| Energy metabolism in nuclear reprogramming. | Folmes CD et al. | — | 2011 | → |
| Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease. | Rada-Iglesias A et al. | — | 2011 | → |
| Human induced pluripotent stem cells differentiation into oligodendrocyte progenitors and transplantation in a rat model of optic chiasm demyelination. | Pouya A et al. | — | 2011 | → |
| Investigating monogenic and complex diseases with pluripotent stem cells. | Zhu H et al. | — | 2011 | → |
| MicroRNAs and reprogramming. | Chang HM et al. | — | 2011 | → |
| miRNA screening reveals a new miRNA family stimulating iPS cell generation via regulation of Meox2. | Pfaff N et al. | — | 2011 | → |
| Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells. | Subramanyam D et al. | — | 2011 | → |
| Progress in understanding reprogramming to the induced pluripotent state. | Plath K et al. | — | 2011 | → |
| Stem cells: The growing pains of pluripotency. | Hayden EC | — | 2011 | → |
| Stem Cell Therapy: A New Treatment for Burns? | Arno A et al. | — | 2011 | → |
| The therapeutic potential of ΦC31 integrase as a gene therapy system. | Karow M et al. | — | 2011 | → |
| Transcriptomic analysis of pluripotent stem cells: insights into health and disease. | Yeo JC et al. | — | 2011 | → |
| MicroRNA gene regulatory pathways in the establishment and maintenance of ESC identity. | Martinez NJ et al. | — | 2010 | → |