Effects of Fatty Acids on CYP2A5 and Nrf2 Expression in Mouse Primary Hepatocytes.
- Authors
- Cui, Yizhe; Wang, Qiuju; Yi, Xing; Zhang, Xiuying
- Year
- 2016
- Journal
- Biochemical genetics
- PMID
- 26423681
- DOI
- 10.1007/s10528-015-9697-6
Abnormal fatty acid metabolism is observed throughout nonalcoholic fatty liver disease (NAFLD) pathogenesis, and fatty acid storage is an important inducing factor in insulin resistance, lipid oxidation, hepatic cell damage, and inflammation. During NAFLD pathogenesis, changes in blood and liver contents of different fatty acid types also vary. Cytochrome P450 2A5 (CYP2A5), an important enzyme in mouse liver, metabolizes many drugs and activates multiple pro-carcinogens with widely varying structures. According to the changes in liver fatty acid profiles observed in NAFLD animal models developed in our laboratory and others, saturated (PA/palmitic, and SA/stearic acids) and unsaturated (OA/oleic, LA/linoleic, ALA/α-linolenic and AA/arachidonic acids) fatty acids were selected to induce mouse primary hepatocytes, at concentrations under 1 mM, as detected by MTT assay. After 24 h treatment with various fatty acid concentrations and types, CYP2A5 mRNA and protein amounts, and enzyme activity were determined by real-time PCR, Western blot, and Coumarin 7-hydroxylation, respectively. Meanwhile, Nrf2 mRNA and protein levels were evaluated by real-time PCR and Western blot. The results indicated that saturated fatty acids are more potent in inducing CYP2A5 than unsaturated ones, except arachidonic acid. In addition, the changes in CYP2A5 expression were consistent with the alterations observed in Nrf2 expression, indicating that Nrf2 might play a regulatory role in CYP2A5 expression.
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External
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| Loss of NRF2 accelerates cognitive decline, exacerbates mitochondrial dysfunction, and is required for the cognitive enhancing effects of Centella asiatica during aging. | Zweig JA et al. | — | 2021 | → |
| CYP2A6 is associated with obesity: studies in human samples and a high fat diet mouse model. | Wang K et al. | — | 2019 | → |
| NDP-MSH reduces oxidative damage induced by palmitic acid in primary astrocytes. | RamÃrez D et al. | — | 2019 | → |
| Cytochrome P450s and Alcoholic Liver Disease. | Lu Y et al. | — | 2018 | → |
| Resveratrol-loaded PLGA nanoparticles: enhanced stability, solubility and bioactivity of resveratrol for non-alcoholic fatty liver disease therapy. | Wan S et al. | — | 2018 | → |
| Chronic administration of saturated fats and fructose differently affect SREBP activity resulting in different modulation of Nrf2 and Nlrp3 inflammasome pathways in mice liver. | Nigro D et al. | — | 2017 | → |
| Comparison between conjugated linoleic acid and essential fatty acids in preventing oxidative stress in bovine mammary epithelial cells. | Basiricò L et al. | — | 2017 | → |
| High Fat Diet-Induced Hepatic 18-Carbon Fatty Acids Accumulation Up-Regulates CYP2A5/CYP2A6 via NF-E2-Related Factor 2. | Wang XH et al. | — | 2017 | → |
| Non-alcoholic fatty liver disease (NAFLD) - pathogenesis, classification, and effect on drug metabolizing enzymes and transporters. | Cobbina E et al. | — | 2017 | → |
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| Resveratrol Ameliorates Palmitate-Induced Inflammation in Skeletal Muscle Cells by Attenuating Oxidative Stress and JNK/NF-κB Pathway in a SIRT1-Independent Mechanism. | Sadeghi A et al. | — | 2017 | → |
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| Induction of Nrf2/ARE-mediated cytoprotective genes by red ginseng oil through ASK1-MKK4/7-JNK and p38 MAPK signaling pathways in HepG2 cells. | Bak MJ et al. | — | 2016 | → |