Human 1A6/DRIM, the homolog of yeast Utp20, functions in the 18S rRNA processing.
- Authors
- Wang, You; Liu, Jiangying; Zhao, Hong; Lü, Wenqing; Zhao, Jun; Yang, Lin; Li, Ning; Du, Xiaojuan; Ke, Yang
- Year
- 2007
- Journal
- Biochimica et biophysica acta
- PMID
- 17498821
- DOI
- 10.1016/j.bbamcr.2007.04.002
1A6/DRIM is a nucleolar protein with a nucleolar targeting sequence in its 3'-terminus. Bioinformatic analysis indicated that human 1A6/DRIM shares 23% identity and 43% similarity with yeast Utp20, which has been reported as a component of U3 snoRNA protein complex and has been implicated in 18S rRNA processing. In the present study, we found, by utilizing RT-PCR with RNA extracted from anti-1A6/DRIM immunoprecipitates and Northern blotting, that 1A6/DRIM is associated with U3 snoRNA. Pulse-chase labeling assays showed that silencing of 1A6/DRIM expression in HeLa cells resulted in a delayed 18S rRNA processing. Furthermore, immunoprecipitations revealed that 1A6/DRIM was also associated with fibrillarin, another U3 RNP component in HeLa cells. These results indicate that 1A6/DRIM is involved in 18S rRNA processing and is the bona fide mammalian Utp20.
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