Evaluation of the glutamate decarboxylase genes Gad1 and Gad2 as candidate genes for acute ethanol withdrawal severity in mice.
- Authors
- Fehr, Christoph; Rademacher, Brooks L S; Buck, Kari J
- Year
- 2003
- Journal
- Progress in neuro-psychopharmacology & biological psychiatry
- PMID
- 12691782
- DOI
- 10.1016/S0278-5846(03)00034-4
Previous studies in crosses between the C57BL/6J (B6) and the DBA/2J (D2) mice have implicated a role of the genes encoding for the 67- and 65-kDa isoforms of the glutamate decarboxylase (Gad1 and Gad2) in the manifestation and severity of multiple ethanol-related traits such as acute ethanol withdrawal severity [Buck, K.J., Metten, P., Belknap, J.K., Crabbe, J.C., 1997. Quantitative trait loci involved in genetic predisposition to acute alcohol withdrawal in mice. J. Neurosci. 17, 3946-3955], ethanol preference [Phillips, T.J., Belknap, J.K., Buck, K.J., Cunningham, C.L., 1998. Genes on mouse chromosomes 2 and 9 determine variation in ethanol consumption. Mamm. Genome 9, 936-941] and ethanol-induced locomotion [Demarest, K., McCaughran Jr., J., Mahjubi, E., Cipp, L., Hitzemann, R., 1999. Identification of an acute ethanol response quantitative trait locus on mouse chromosome 2. J. Neurosci. 19, 549-561]. Strain-specific sequencing experiments as well as gene expression studies in drug-naive and ethanol-treated D2 and B6 mice were carried out. The Gad1 sequence was similar, the Gad2 cDNA carried only a silent polymorphism (1017 G>C) between both strains. In addition, no significant GAD65 or GAD67 expression differences were detected in either drug-nai;ve or acute ethanol withdrawn animals by Western blot experiments. Therefore, these results do not support the hypothesis of an involvement of Gad1 or Gad2 in the pathophysiology of acute ethanol withdrawal severity and the other ethanol related traits.
No figures extracted from this document.
No chunks — full text not yet ingested.
No entities extracted from this document yet.
No uploaded files.
No citations found.
In this knowledge base
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Genetic Inactivation of the Serotonin Transporter Dysregulates Expression of Neurotransmission Genes and Genome-Wide DNA Methylation Levels in the Medial Prefrontal Cortex of Male Rats During Postnatal Development. | Kroeze Y et al. | — | 2025 | → |
| GABAergic mechanisms in alcohol dependence. | Uusi-Oukari M et al. | — | 2024 | → |
| Thermal antinociceptive responses to alcohol in DBA/2J and C57BL/6J inbred male and female mouse strains. | White A et al. | — | 2023 | → |
| Analyses of differentially expressed genes after exposure to acute stress, acute ethanol, or a combination of both in mice. | Baker JA et al. | — | 2017 | → |
| Identifying genetic variation for alcohol dependence. | Agrawal A et al. | — | 2012 | → |
| Mice lacking Gad2 show altered behavioral effects of ethanol, flurazepam and gabaxadol. | Blednov YA et al. | — | 2010 | → |
| Associations of glutamate decarboxylase genes with initial sensitivity and age-at-onset of alcohol dependence in the Irish Affected Sib Pair Study of Alcohol Dependence. | Kuo PH et al. | — | 2009 | → |
| Linkage scan for quantitative traits identifies new regions of interest for substance dependence in the Collaborative Study on the Genetics of Alcoholism (COGA) sample. | Agrawal A et al. | — | 2008 | → |
| Glutamate decarboxylase genes and alcoholism in Han Taiwanese men. | Loh el-W et al. | — | 2006 | → |
| Isolation and confirmation of a calcium excretion quantitative trait locus on chromosome 1 in genetic hypercalciuric stone-forming congenic rats. | Hoopes RR et al. | — | 2006 | → |
| Genetic correlational analyses of ethanol reward and aversion phenotypes in short-term selected mouse lines bred for ethanol drinking or ethanol-induced conditioned taste aversion. | Phillips TJ et al. | — | 2005 | → |
| The syntaxin binding protein 1 gene (Stxbp1) is a candidate for an ethanol preference drinking locus on mouse chromosome 2. | Fehr C et al. | — | 2005 | → |
| Transcriptome analysis of frontal cortex in alcohol-preferring and nonpreferring rats. | Worst TJ et al. | — | 2005 | → |