Skin toxicity determined in vitro by three-dimensional, native-state histoculture.
- Authors
- Li, L N; Margolis, L B; Hoffman, R M
- Year
- 1991
- Journal
- Proceedings of the National Academy of Sciences of the United States of America
- PMID
- 1848016
- DOI
- 10.1073/pnas.88.5.1908
- PMCID
- PMC51135
We describe a gel-supported in vitro system for culturing skin samples in a three-dimensional native state. All the cell types of skin remain viable and maintain their native architecture for at least 10 days. The culture system is used for toxicity measurements by ascertaining cell viability using two fluorescent dyes: 2',7'-bis-(2-carboxyethyl)-5-(and -6)carboxyfluorescein acetoxymethyl ester, specific for living cells, and propidium iodide, specific for dead cells. Cell staining with the dyes is measured throughout the tissue block by confocal scanning fluorescence microscopy. The dose-response to three agents--ethanol, doxorubicin, and sodium hypochlorite--is shown and, in the case of sodium hypochlorite, compared to in vivo skin toxicity with a high correlation. We also demonstrate that the end point of [3H]thymidine incorporation measured by histological autoradiography can be used to measure toxicity. Our results with the [3H]thymidine end point demonstrate that the hair follicle cells are the most sensitive to doxorubicin. The native-state model for skin may be an effective replacement for animal systems and superior to the dispersed skin cell systems used previously. It can allow rapid, inexpensive measurements of the effect of manufactured products, drugs, and pollutants on skin.
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External
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|---|---|---|---|---|
| Skin Irritation Testing beyond Tissue Viability: Fucoxanthin Effects on Inflammation, Homeostasis, and Metabolism. | Spagolla Napoleão Tavares R et al. | — | 2020 | → |
| Using human stem cells as a model system to understand the neural mechanisms of alcohol use disorders: Current status and outlook. | Scarnati MS et al. | — | 2019 | → |
| 3D Sponge-Matrix Histoculture: An Overview. | Hoffman RM | — | 2018 | → |
| Icotinib inhibits EGFR signaling and alleviates psoriasis-like symptoms in animal models. | Tan F et al. | — | 2018 | → |
| Ex vivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis. | Lebedeva A et al. | — | 2017 | → |
| Introduction to Hair-Follicle-Associated Pluripotent Stem Cells. | Hoffman RM | — | 2016 | → |
| Comparison of label-free and GFP multiphoton imaging of hair follicle-associated pluripotent (HAP) stem cells in mouse whiskers. | Uchugonova A et al. | — | 2015 | → |
| Histone H3 Phosphorylation in Human Skin Histoculture as a Tool to Evaluate Patient's Response to Antiproliferative Drugs. | Ugarte F et al. | — | 2015 | → |
| Pumpless microfluidic platform for drug testing on human skin equivalents. | Abaci HE et al. | — | 2015 | → |
| Skin equivalents: skin from reconstructions as models to study skin development and diseases. | Ali N et al. | — | 2015 | → |
| Nestin-expressing hair follicle-accessible pluripotent stem cells for nerve and spinal cord repair. | Hoffman RM | — | 2014 | → |
| Ex vivo evaluation of imatinib mesylate for induction of cell death on canine neoplastic mast cells with mutations in c-Kit exon 11 via apoptosis. | Rossi G et al. | — | 2013 | → |
| The bulge area is the origin of nestin-expressing pluripotent stem cells of the hair follicle. | Uchugonova A et al. | — | 2011 | → |
| [Development of alternative to animal experiment in evaluation of skin irritation caused by alcohol-based hand rubs]. | Yamamoto N et al. | — | 2010 | → |
| A human folliculoid microsphere assay for exploring epithelial- mesenchymal interactions in the human hair follicle. | Havlickova B et al. | — | 2009 | → |
| In vitro and in vivo models of acute alcohol exposure. | Dolganiuc A et al. | — | 2009 | → |
| The hair follicle and its stem cells as drug delivery targets. | Hoffman RM | — | 2006 | → |
| Replication of Aleutian mink disease parvovirus in mink lymph node histocultures. | Jensen KT et al. | — | 2000 | → |
| Genistein inhibits the growth of human-patient BPH and prostate cancer in histoculture. | Geller J et al. | — | 1998 | → |
| Topical liposome targeting of dyes, melanins, genes, and proteins selectively to hair follicles. | Hoffman RM | — | 1998 | → |
| Comparison of androgen-independent growth and androgen-dependent growth in BPH and cancer tissue from the same radical prostatectomies in sponge-gel matrix histoculture. | Geller J et al. | — | 1997 | → |
| Chondrocyte survival in cryopreserved osteochondral articular cartilage. | Ohlendorf C et al. | — | 1996 | → |
| Confocal microscopy of cells implanted into tissue blocks: cell migration in long-term histocultures. | Margolis LB et al. | — | 1995 | → |
| Dye-coupling in three-dimensional histoculture of rat lingual frenulum. | Margolis L et al. | — | 1995 | → |
| Model of selective gene therapy of hair growth: liposome targeting of the active Lac-Z gene to hair follicles of histocultured skin. | Li L et al. | — | 1995 | → |
| Syncytium formation in cultured human lymphoid tissue: fusion of implanted HIV glycoprotein 120/41-expressing cells with native CD4+ cells. | Margolis LB et al. | — | 1995 | → |
| The feasibility of targeted selective gene therapy of the hair follicle. | Li L et al. | — | 1995 | → |
| Growth of wool follicles in culture. | Bond JJ et al. | — | 1994 | → |
| High efficiency liposome-mediated transfection of the tyrosinase gene to cultured cells: a model for the gene therapy of hair color restoration. | Li L et al. | — | 1994 | → |
| Histoculture radiometric in vitro hair growth assay. | Li L et al. | — | 1993 | → |
| Liposomes can specifically target entrapped melanin to hair follicles in histocultured skin. | Li L et al. | — | 1993 | → |
| Liposome targeting of high molecular weight DNA to the hair follicles of histocultured skin: a model for gene therapy of the hair growth processes. | Li L et al. | — | 1993 | → |
| Hair growth in vitro from histocultured skin. | Li L et al. | — | 1992 | → |
| Hair shaft elongation, follicle growth, and spontaneous regression in long-term, gelatin sponge-supported histoculture of human scalp skin. | Li L et al. | — | 1992 | → |
| Long-term passage of human tissues in vitro as three-dimensional histolines. | Slocum HK et al. | — | 1992 | → |
| Product-delivering liposomes specifically target hair follicles in histocultured intact skin. | Li L et al. | — | 1992 | → |
| Skin histoculture assay for studying the hair cycle. | Li L et al. | — | 1992 | → |
| Hair growth and hair follicle cell proliferation in histocultured mouse skin. | Li L et al. | — | 1991 | → |
| In vivo growth patterns of human lung tumors in three-dimensional histoculture. | Baibakov B et al. | — | 1991 | → |