Elevated prodynorphin expression associated with ethanol withdrawal convulsions.
- Authors
- Beadles-Bohling, A S; Crabbe, J C; Wiren, K M
- Year
- 2000
- Journal
- Neurochemistry international
- PMID
- 10871698
- DOI
- 10.1016/s0197-0186(00)00056-5
The hypothesis that kappa-opioid system activity may in part mediate convulsions exhibited during ethanol withdrawal was tested by exposing Withdrawal Seizure-Prone (WSP) and Withdrawal Seizure-Resistant (WSR) mice to chronic ethanol. Whole brain was harvested for RNA isolation and prodynorphin mRNA steady-state levels in whole brain were examined using Northern blot analysis. The data revealed significantly increased levels of prodynorphin mRNA expression in mice susceptible to ethanol withdrawal convulsions after withdrawal, with no corresponding increase in prodynorphin steady-state levels in mice resistant to ethanol withdrawal convulsions. These findings were not due to basal differences in prodynorphin expression between the WSP and WSR mice. To verify that the differences observed were not due to an ethanol-induced global alteration in gene transcription, mRNA levels of the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase were measured. Glyceraldehyde-3-phosphate dehydrogenase expression was unchanged following both chronic exposure to ethanol and chronic exposure followed by withdrawal. These results extend our understanding of prodynorphin's role in generalized seizure activity to include ethanol withdrawal-induced convulsions. Our findings suggest that prodynorphin expression is modulated during ethanol withdrawal convulsions, or alternatively, prodynorphin may mediate the severity of ethanol withdrawal convulsions.
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External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Role of the Dynorphin/Kappa Opioid Receptor System in the Motivational Effects of Ethanol. | Anderson RI et al. | — | 2017 | → |
| Neurochemical mechanisms of alcohol withdrawal. | Becker HC et al. | — | 2014 | → |
| Neuropeptide modulation of central amygdala neuroplasticity is a key mediator of alcohol dependence. | Gilpin NW et al. | — | 2012 | → |
| A regulatory variation in OPRK1, the gene encoding the kappa-opioid receptor, is associated with alcohol dependence. | Edenberg HJ et al. | — | 2008 | → |
| Dynorphin is a downstream effector of striatal BDNF regulation of ethanol intake. | Logrip ML et al. | — | 2008 | → |
| Anticonvulsive effects of kappa-opioid receptor modulation in an animal model of ethanol withdrawal. | Beadles-Bohling AS et al. | — | 2006 | → |
| Alcohol and gene expression in the central nervous system. | Worst TJ et al. | — | 2005 | → |
| Alcohol effects on central nervous system gene expression in genetic animal models. | McBride WJ et al. | — | 2005 | → |
| Alteration of kappa-opioid receptor system expression in distinct brain regions of a genetic model of enhanced ethanol withdrawal severity. | Beadles-Bohling AS et al. | — | 2005 | → |
| Increased sensitivity to NMDA is involved in alcohol-withdrawal induced cytotoxicity observed in primary cultures of cortical neurones chronically pre-treated with ethanol. | Nagy J et al. | — | 2002 | → |
| Ethanol-regulated gene expression of neuroendocrine specific protein in mice: brain region and genotype specificity. | Schafer GL et al. | — | 2001 | → |