MAGI-1 acts as a scaffolding molecule for NGF receptor-mediated signaling pathway.
- Authors
- Ito, Hidenori; Morishita, Rika; Iwamoto, Ikuko; Mizuno, Makoto; Nagata, Koh-ichi
- Year
- 2013
- Journal
- Biochimica et biophysica acta
- PMID
- 23769981
- DOI
- 10.1016/j.bbamcr.2013.06.005
We have recently found that the membrane-associated guanylate kinase with inverted organization-1 (MAGI-1) was enriched in rat nervous tissues such as the glomeruli in olfactory bulb of adult rats and dorsal root entry zone in spinal cord of embryonic rats. In addition, we revealed the localization of MAGI-1 in the growth cone of the primary cultured rat dorsal root ganglion cells. These results point out the possibility that MAGI-1 is involved in the regulation of neurite extension or guidance. In this study, we attempted to reveal the physiological role(s) of MAGI-1 in neurite extension. We found that RNA interference (RNAi)-mediated knockdown of MAGI-1 caused inhibition of nerve growth factor (NGF)-induced neurite outgrowth in PC12 rat pheochromocytoma cells. To clarify the involvement of MAGI-1 in NGF-mediated signal pathway, we tried to identify binding partners for MAGI-1 and identified p75 neurotrophin receptor (p75NTR), a low affinity NGF receptor, and Shc, a phosphotyrosine-binding adaptor. These three proteins formed an immunocomplex in PC12 cells. Knockdown as well as overexpression of MAGI-1 caused suppression of NGF-stimulated activation of the Shc-ERK pathway, which is supposed to play important roles in neurite outgrowth of PC12 cells. These results indicate that MAGI-1 may act as a scaffolding molecule for NGF receptor-mediated signaling pathway.
No figures extracted from this document.
No chunks — full text not yet ingested.
No entities extracted from this document yet.
No uploaded files.
No citations found.
In this knowledge base
| Title | Year | PMID |
|---|---|---|
| Meta-analysis of Genome-wide Association Studies for Neuroticism, and the Polygenic Association With Major Depressive Disorder. | 2015 | 25993607 |
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| A Potential Role for MAGI-1 in the Bi-Directional Relationship Between Major Depressive Disorder and Cardiovascular Disease. | Banerjee P et al. | — | 2024 | → |
| The magic of MAGI-1: A scaffolding protein with multi signalosomes and functional plasticity. | Excoffon KJDA et al. | — | 2022 | → |
| A New Story of the Three Magi: Scaffolding Proteins and lncRNA Suppressors of Cancer. | Kotelevets L et al. | — | 2021 | → |
| Novel phospho-switch function of delta-catenin in dendrite development. | Baumert R et al. | — | 2020 | → |
| Meta-analysis of Genome-wide Association Studies for Neuroticism, and the Polygenic Association With Major Depressive Disorder. | Genetics of Personality Consortium et al. | — | 2015 | → |
| Role of p75 neurotrophin receptor in stem cell biology: more than just a marker. | Tomellini E et al. | — | 2014 | → |