Stoichiometry of a ligand-gated ion channel determined by fluorescence energy transfer.
paper
Cited
Public
Unavailable
- Authors
- Farrar, S J; Whiting, P J; Bonnert, T P; McKernan, R M
- Year
- 1999
- Journal
- The Journal of biological chemistry
- PMID
- 10187791
- DOI
- 10.1074/jbc.274.15.10100
We have developed a method to determine the stoichiometry of subunits within an oligomeric cell surface receptor using fluorescently tagged antibodies to the individual subunits and measuring energy transfer between them. Anti-c-Myc monoclonal antibody (mAb 9-E10) derivatized with a fluorophore (europium cryptate, EuK) was used to individually label c-Myc-tagged alpha1-, beta2-, or gamma2-subunits of the hetero-oligomeric gamma-aminobutyric acid (GABAA) receptor in intact cells. The maximal fluorescent signal derived from the alpha1(c-Myc)beta2gamma2 and the alpha1beta2(c-Myc)gamma2 receptors was twice that obtained with alpha1beta2gamma2(c-Myc), suggesting that there are 2x alpha-, 2x beta-, and 1x gamma-subunits in a receptor monomer. This observation was extended using fluorescence energy transfer. Receptors were half-maximally saturated with EuK-anti-c-Myc mAb, and the remaining alpha1(c-Myc) subunits were labeled with excess anti-c-Myc mAb derivatized with the fluorescence energy acceptor, XL665. On exposure to laser light, energy transfer from EuK to XL665 occurred with alpha1(c-Myc)beta2gamma2 and alpha1beta2(c-Myc)gamma2, but no significant energy transfer was observed with alpha1beta2gamma2(c-Myc) receptors, indicating the absence of a second gamma-subunit in a receptor monomer. We confirm that the GABAA receptor subtype, alpha1beta2gamma2, is composed of two copies each of the alpha- and beta-subunits and one copy of the gamma-subunit (i.e. (alpha1)2(beta2)2(gamma2)1) and conclude that this method would have general applicability to other multisubunit cell surface proteins.
No figures extracted from this document.
No chunks β full text not yet ingested.
No entities extracted from this document yet.
No uploaded files.
Not in any collection.
No citations found.
In this knowledge base
| Title | Year | PMID |
|---|---|---|
| Altering the relative abundance of GABA A receptor subunits changes GABA- and ethanol-responses in Xenopus oocytes. | 2009 | 19382902 |
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Effects of progestogen neurosteroids on locomotor activity in zebrafish embryos and larvae. | Mathouchanh M et al. | β | 2025 | β |
| GABA<sub>A</sub> receptor Ο forms channels that stimulate ERK through a G-protein-dependent pathway. | Wang Y et al. | β | 2025 | β |
| Propofol reduces human TRPA1 activity in a warm environment. | Suda C et al. | β | 2025 | β |
| Physiological Function of RDL1 and RDL2 Subunits of the Ionotropic GABA Receptor in the <i>Spodoptera litura</i> with the CRISPR/Cas9 System <i>In Vivo</i>. | Jia ZQ et al. | β | 2023 | β |
| Correlation of receptor density and mRNA expression patterns in the human cerebral cortex. | MurgaΕ‘ M et al. | β | 2022 | β |
| GABA receptor associated protein changes the electrostatic environment around the GABA type A receptor. | Irwin BWJ et al. | β | 2022 | β |
| Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters. | George S et al. | β | 2022 | β |
| Characterization of zebrafish GABA<sub>A</sub> receptor subunits. | Sadamitsu K et al. | β | 2021 | β |
| Correlation of receptor density and mRNA expression patterns in the human cerebral cortex | Matej M et al. | β | 2021 | β |
| Mechanisms of GABAergic and cholinergic neurotransmission in auditory thalamus: Impact of aging. | Richardson BD et al. | β | 2021 | β |
| Development of 1,3-thiazole analogues of imidazopyridines as potent positive allosteric modulators of GABA<sub>A</sub> receptors. | Tikhonova TA et al. | β | 2020 | β |
| GABA<sub>A</sub> Receptor Ξ²<sub>2</sub>E155 Residue Located at the Agonist-Binding Site Is Involved in the Receptor Gating. | Jatczak-Εliwa M et al. | β | 2020 | β |
| Mutations of Ξ±<sub>1</sub>F45 residue of GABA<sub>A</sub> receptor loop G reveal its involvement in agonist binding and channel opening/closing transitions. | Brodzki M et al. | β | 2020 | β |
| Extrasynaptic Ξ΄-GABA<sub>A</sub> receptors are high-affinity muscimol receptors. | Benkherouf AY et al. | β | 2019 | β |
| Ξ-Aminobutyric acid in adult brain: an update. | Siucinska E | β | 2019 | β |
| Comparison of Ξ±Ξ²Ξ΄ and Ξ±Ξ²Ξ³ GABA<sub>A</sub> receptors: Allosteric modulation and identification of subunit arrangement by site-selective general anesthetics. | Feng HJ et al. | β | 2018 | β |
| Cryo-EM structure of the benzodiazepine-sensitive Ξ±1Ξ²1Ξ³2S tri-heteromeric GABA<sub>A</sub> receptor in complex with GABA. | Phulera S et al. | β | 2018 | β |
| Developmental Changes in Serotonergic Modulation of GABAergic Synaptic Transmission and Postsynaptic GABA<sub>A</sub> Receptor Composition in the Cerebellar Nuclei. | Saitow F et al. | β | 2018 | β |
| Expression and purification of a functional heteromeric GABAA receptor for structural studies. | Claxton DP et al. | β | 2018 | β |
| GABA beyond the synapse: defining the subtype-specific pharmacodynamics of non-synaptic GABA<sub>A</sub> receptors. | Lagrange AH et al. | β | 2018 | β |
| Neurotransmitter receptors as signaling platforms in anterior pituitary cells. | ZemkovΓ‘ H et al. | β | 2018 | β |
| NK1R/5-HT1AR interaction is related to the regulation of melanogenesis. | Wu H et al. | β | 2018 | β |
| Nuclear Respiratory Factor 1 (NRF-1) Controls the Activity Dependent Transcription of the GABA-A Receptor Beta 1 Subunit Gene in Neurons. | Li Z et al. | β | 2018 | β |
| Synthesis of C-14 labeled GABA<sub>A</sub> Ξ±2/Ξ±3 selective partial agonists and the investigation of late-occurring and long-circulating metabolites of GABA<sub>A</sub> receptor modulator AZD7325. | Artelsmair M et al. | β | 2018 | β |
| Binding site opening by loop C shift and chloride ion-pore interaction in the GABA<sub>A</sub> receptor model. | MichaΕowski MA et al. | β | 2017 | β |
| GABA<sub>A</sub> Receptors and the Diversity in their Structure and Pharmacology. | Chua HC et al. | β | 2017 | β |
| Inhibitory synapse deficits caused by familial Ξ±1 GABA<sub>A</sub> receptor mutations in epilepsy. | Chen X et al. | β | 2017 | β |
| Low Expression in Xenopus Oocytes and Unusual Functional Properties of Ξ±1Ξ²2Ξ³2 GABAA Receptors with Non-Conventional Subunit Arrangement. | Baur R et al. | β | 2017 | β |
| Role of the amygdala GABA-A receptors in ACPA-induced deficits during conditioned fear learning. | Nasehi M et al. | β | 2017 | β |
| Towards a Better Understanding of GABAergic Remodeling in Alzheimer's Disease. | Govindpani K et al. | β | 2017 | β |
| Ξ± subunits in GABA<sub>A</sub> receptors are dispensable for GABA and diazepam action. | Wongsamitkul N et al. | β | 2017 | β |
| A new chromanone derivative isolated from Hypericum lissophloeus (Hypericaceae) potentiates GABAA receptor currents in a subunit specific fashion. | Crockett S et al. | β | 2016 | β |
| Comparison of kinetic and pharmacological profiles of recombinant Ξ±1Ξ³2L and Ξ±1Ξ²2Ξ³2L GABAA receptors - A clue to the role of intersubunit interactions. | Brodzki M et al. | β | 2016 | β |
| Functional sites involved in modulation of the GABAA receptor channel by the intravenous anesthetics propofol, etomidate and pentobarbital. | Maldifassi MC et al. | β | 2016 | β |
| Novel positive allosteric modulators of GABAA receptors with anesthetic activity. | Maldifassi MC et al. | β | 2016 | β |
| Revealing Higher Order Protein Structure Using Mass Spectrometry. | Chait BT et al. | β | 2016 | β |
| RNA Editing: A Contributor to Neuronal Dynamics in the Mammalian Brain. | Behm M et al. | β | 2016 | β |
| Toward Understanding Functional Properties and Subunit Arrangement of Ξ±4Ξ²2Ξ΄ Ξ³-Aminobutyric Acid, Type A (GABAA) Receptors. | Wongsamitkul N et al. | β | 2016 | β |
| To what extent is it possible to dissociate the anxiolytic and sedative/hypnotic properties of GABAA receptors modulators? | Chagraoui A et al. | β | 2016 | β |
| Zolpidem is a potent stoichiometry-selective modulator of Ξ±1Ξ²3 GABAA receptors: evidence of a novel benzodiazepine site in the Ξ±1-Ξ±1 interface. | Che Has AT et al. | β | 2016 | β |
| GABA receptor subunit distribution and FMRP-mGluR5 signaling abnormalities in the cerebellum of subjects with schizophrenia, mood disorders, and autism. | Fatemi SH et al. | β | 2015 | β |
| Electroneutral absorption of NaCl by the aldosterone-sensitive distal nephron: implication for normal electrolytes homeostasis and blood pressure regulation. | Eladari D et al. | β | 2014 | β |
| Moderate concentrations of 4-O-methylhonokiol potentiate GABAA receptor currents stronger than honokiol. | Baur R et al. | β | 2014 | β |
| Phosphorylation of GABAA receptors influences receptor trafficking and neurosteroid actions. | Comenencia-Ortiz E et al. | β | 2014 | β |
| Relative positioning of classical benzodiazepines to the Ξ³2-subunit of GABAA receptors. | Middendorp SJ et al. | β | 2014 | β |
| The role of ovarian hormone-derived neurosteroids on the regulation of GABAA receptors in affective disorders. | MacKenzie G et al. | β | 2014 | β |
| Unexpected Properties of Ξ΄-Containing GABAA Receptors in Response to Ligands Interacting with the Ξ±+ Ξ²- Site. | Mirheydari P et al. | β | 2014 | β |
| Do N-arachidonyl-glycine (NA-glycine) and 2-arachidonoyl glycerol (2-AG) share mode of action and the binding site on the Ξ²2 subunit of GABAA receptors? | Baur R et al. | β | 2013 | β |
| GABA<sub>A</sub> Receptors of Cerebellar Granule Cells in Culture: Interaction with Benzodiazepines. | Cupello A et al. | β | 2013 | β |
| Identification of novel positive allosteric modulators and null modulators at the GABAA receptor Ξ±+Ξ²- interface. | Varagic Z et al. | β | 2013 | β |
| Molecular analysis of the site for 2-arachidonylglycerol (2-AG) on the Ξ²β subunit of GABA(A) receptors. | Baur R et al. | β | 2013 | β |
| Transcriptional changes in rat Ξ±-motoneurons resulting from increased physical activity. | Woodrow L et al. | β | 2013 | β |
| What is special about the adolescent (JME) brain? | Craiu D | β | 2013 | β |
| Aging impairs the control of prefrontal cortex on the release of corticosterone in response to stress and on memory consolidation. | Garrido P et al. | β | 2012 | β |
| A novel GABA(A) receptor pharmacology: drugs interacting with the Ξ±(+) Ξ²(-) interface. | Sieghart W et al. | β | 2012 | β |
| Influence of GABA(A) receptor Ξ± subunit isoforms on the benzodiazepine binding site. | LΓΌscher BP et al. | β | 2012 | β |
| Orai1 determines calcium selectivity of an endogenous TRPC heterotetramer channel. | Cioffi DL et al. | β | 2012 | β |
| Adenosine-to-inosine RNA editing affects trafficking of the gamma-aminobutyric acid type A (GABA(A)) receptor. | Daniel C et al. | β | 2011 | β |
| Augmentation of Tonic GABA(A) Inhibition in Absence Epilepsy: Therapeutic Value of Inverse Agonists at Extrasynaptic GABA(A) Receptors. | Errington AC et al. | β | 2011 | β |
| Differential localization of GABA(A) receptor subunits in relation to rat striatopallidal and pallidopallidal synapses. | Gross A et al. | β | 2011 | β |
| Different residues in the GABAA receptor benzodiazepine binding pocket mediate benzodiazepine efficacy and binding. | Morlock EV et al. | β | 2011 | β |
| Emerging themes in GABAergic synapse development. | Kuzirian MS et al. | β | 2011 | β |
| GABA(A) receptor and glycine receptor activation by paracrine/autocrine release of endogenous agonists: more than a simple communication pathway. | Le-Corronc H et al. | β | 2011 | β |
| GABA Potency at GABA(A) Receptors Found in Synaptic and Extrasynaptic Zones. | Mortensen M et al. | β | 2011 | β |
| Implications for treatment: GABAA receptors in aging, Down syndrome and Alzheimer's disease. | Rissman RA et al. | β | 2011 | β |
| Neurosteroids and GABA-A Receptor Function. | Wang M | β | 2011 | β |
| The dynamic modulation of GABA(A) receptor trafficking and its role in regulating the plasticity of inhibitory synapses. | Vithlani M et al. | β | 2011 | β |
| The GABAA receptor alpha+beta- interface: a novel target for subtype selective drugs. | Ramerstorfer J et al. | β | 2011 | β |
| Benzodiazepine modulation of the rat GABAA receptor Ξ±4Ξ²3Ξ³2L subtype expressed in Xenopus oocytes. | You H et al. | β | 2010 | β |
| New insights into the molecular mechanisms of general anaesthetics. | Chau PL | β | 2010 | β |
| Stoichiometry of expressed alpha(4)beta(2)delta gamma-aminobutyric acid type A receptors depends on the ratio of subunit cDNA transfected. | Wagoner KR et al. | β | 2010 | β |
| Altering the relative abundance of GABA A receptor subunits changes GABA- and ethanol-responses in Xenopus oocytes. | Hurley JH et al. | β | 2009 | β |
| Effects of the beta-amino acid antagonist TAG on thalamocortical inhibition. | Mathers DA et al. | β | 2009 | β |
| Homology modeling of human alpha 1 beta 2 gamma 2 and house fly beta 3 GABA receptor channels and Surflex-docking of fipronil. | Cheng J et al. | β | 2009 | β |
| Mechanism of bis(7)-tacrine inhibition of GABA-activated current in cultured rat hippocampal neurons. | Zhou L et al. | β | 2009 | β |
| Relative positioning of diazepam in the benzodiazepine-binding-pocket of GABA receptors. | Tan KR et al. | β | 2009 | β |
| Atomic force microscopy reveals the stoichiometry and subunit arrangement of the alpha4beta3delta GABA(A) receptor. | Barrera NP et al. | β | 2008 | β |
| Determination of the architecture of ionotropic receptors using AFM imaging. | Barrera NP et al. | β | 2008 | β |
| GABA(A) Receptor Dynamics and Constructing GABAergic Synapses. | Tretter V et al. | β | 2008 | β |
| GAB(A) receptors present higher affinity and modified subunit composition in spinal motor neurons from a genetic model of amyotrophic lateral sclerosis. | Carunchio I et al. | β | 2008 | β |
| Molecular, pharmacological and functional properties of GABA(A) receptors in anterior pituitary cells. | Zemkova HW et al. | β | 2008 | β |
| Neurochemical and physiological correlates of a critical period of respiratory development in the rat. | Wong-Riley MT et al. | β | 2008 | β |
| The subunit arrangement and assembly of ionotropic receptors. | Barrera NP et al. | β | 2008 | β |
| Allosteric uncoupling and up-regulation of benzodiazepine and GABA recognition sites following chronic diazepam treatment of HEK 293 cells stably transfected with alpha1beta2gamma2S subunits of GABA (A) receptors. | PericiΔ D et al. | β | 2007 | β |
| A single point mutation of the GABA(A) receptor alpha5-subunit confers fluoxetine sensitivity. | Derry JM et al. | β | 2007 | β |
| Emerging roles for ubiquitin and protein degradation in neuronal function. | Yi JJ et al. | β | 2007 | β |
| Enhanced macroscopic desensitization shapes the response of alpha4 subtype-containing GABAA receptors to synaptic and extrasynaptic GABA. | Lagrange AH et al. | β | 2007 | β |
| Exploring ligand recognition and ion flow in comparative models of the human GABA type A receptor. | Mokrab Y et al. | β | 2007 | β |
| GABA(A) receptors in aging and Alzheimer's disease. | Rissman RA et al. | β | 2007 | β |
| GABAA receptors in the thalamus: alpha4 subunit expression and alcohol sensitivity. | Jia F et al. | β | 2007 | β |
| Identification of a domain in the delta subunit (S238-V264) of the alpha4beta3delta GABAA receptor that confers high agonist sensitivity. | You H et al. | β | 2007 | β |
| Novel 3-iodo-8-ethoxypyrazolo[5,1-c][1,2,4]benzotriazine 5-oxide as promising lead for design of alpha5-inverse agonist useful tools for therapy of mnemonic damage. | Guerrini G et al. | β | 2007 | β |
| Postnatal alterations of the inhibitory synaptic responses recorded from cortical pyramidal neurons in the Lis1/sLis1 mutant mouse. | ValdΓ©s-SΓ‘nchez L et al. | β | 2007 | β |
| Proximity-accelerated chemical coupling reaction in the benzodiazepine-binding site of gamma-aminobutyric acid type A receptors: superposition of different allosteric modulators. | Tan KR et al. | β | 2007 | β |
| Spontaneous cross-link of mutated alpha1 subunits during GABA(A) receptor assembly. | Sarto-Jackson I et al. | β | 2007 | β |
| The cellular, molecular and ionic basis of GABA(A) receptor signalling. | Farrant M et al. | β | 2007 | β |
| Two neighboring residues of loop A of the alpha1 subunit point towards the benzodiazepine binding site of GABAA receptors. | Tan KR et al. | β | 2007 | β |
| 2,3,7-Trisubstituted pyrazolo[1,5-d][1,2,4]triazines: functionally selective GABAA alpha3-subtype agonists. | Carling RW et al. | β | 2006 | β |
| A GABA(A) receptor of defined subunit composition and positioning: concatenation of five subunits. | Baur R et al. | β | 2006 | β |
| CaMK-II modulation of GABA(A) receptors expressed in HEK293, NG108-15 and rat cerebellar granule neurons. | Houston CM et al. | β | 2006 | β |
| Developmental changes in the expression of GABAA receptor subunits alpha1, alpha2, and alpha3 in brain stem nuclei of rats. | Liu Q et al. | β | 2006 | β |
| Identification of amino acid residues important for assembly of GABA receptor alpha1 and gamma2 subunits. | Sarto-Jackson I et al. | β | 2006 | β |
| Imidazo[1,2-a]pyrazin-8-ones, imidazo[1,2-d][1,2,4]triazin-8-ones and imidazo[2,1-f][1,2,4]triazin-8-ones as alpha2/alpha3 subtype selective GABA A agonists for the treatment of anxiety. | Goodacre SC et al. | β | 2006 | β |
| Imidazo[1,2-a]pyrimidines as functionally selective and orally bioavailable GABA(A)alpha2/alpha3 binding site agonists for the treatment of anxiety disorders. | Goodacre SC et al. | β | 2006 | β |
| Local and global ligand-induced changes in the structure of the GABA(A) receptor. | Muroi Y et al. | β | 2006 | β |
| Molecular organization and assembly of the central inhibitory postsynapse. | Arancibia-Carcamo IL et al. | β | 2006 | β |
| Pregnenolone sulphate and Zn2+ inhibit recombinant rat GABA(A) receptor through different channel property. | Wang MD et al. | β | 2006 | β |
| Sites in TM2 and 3 are critical for alcohol-induced conformational changes in GABA receptors. | Jung S et al. | β | 2006 | β |
| Structure, pharmacology, and function of GABAA receptor subtypes. | Sieghart W | β | 2006 | β |
| 7-(1,1-Dimethylethyl)-6-(2-ethyl-2H-1,2,4-triazol-3-ylmethoxy)-3-(2-fluorophenyl)-1,2,4-triazolo[4,3-b]pyridazine: a functionally selective gamma-aminobutyric acid(A) (GABA(A)) alpha2/alpha3-subtype selective agonist that exhibits potent anxiolytic activity but is not sedating in animal models. | Carling RW et al. | β | 2005 | β |
| Atomic force microscopy imaging demonstrates that P2X2 receptors are trimers but that P2X6 receptor subunits do not oligomerize. | Barrera NP et al. | β | 2005 | β |
| Atomic force microscopy reveals the stoichiometry and subunit arrangement of 5-HT3 receptors. | Barrera NP et al. | β | 2005 | β |
| Benzodiazepines affect channel opening of GABA A receptors induced by either agonist binding site. | Baur R et al. | β | 2005 | β |
| Conformational changes at benzodiazepine binding sites of GABA(A) receptors detected with a novel technique. | Berezhnoy D et al. | β | 2005 | β |
| Consequence of the presence of two different beta subunit isoforms in a GABA(A) receptor. | Boulineau N et al. | β | 2005 | β |
| Differential GABAA receptor clustering determines GABA synapse plasticity in rat oxytocin neurons around parturition and the onset of lactation. | Koksma JJ et al. | β | 2005 | β |
| Dynamic mobility of functional GABAA receptors at inhibitory synapses. | Thomas P et al. | β | 2005 | β |
| Enhancement of benzodiazepine binding sites following chronic treatment with flumazenil. | PericiΔ D et al. | β | 2005 | β |
| Functional and structural analysis of the GABAA receptor alpha 1 subunit during channel gating and alcohol modulation. | Jung S et al. | β | 2005 | β |
| Influence of the point mutation alpha-1-H101R on the assembly of gamma-aminobutyric acid type A receptors. | Baur R et al. | β | 2005 | β |
| Mice lacking the major adult GABAA receptor subtype have normal number of synapses, but retain juvenile IPSC kinetics until adulthood. | Bosman LW et al. | β | 2005 | β |
| Novel plant substances acting as beta subunit isoform-selective positive allosteric modulators of GABAA receptors. | Baur R et al. | β | 2005 | β |
| Proton modulation of recombinant GABA(A) receptors: influence of GABA concentration and the beta subunit TM2-TM3 domain. | Wilkins ME et al. | β | 2005 | β |
| Variations on an inhibitory theme: phasic and tonic activation of GABA(A) receptors. | Farrant M et al. | β | 2005 | β |
| 3,4-Dihydronaphthalen-1(2H)-ones: novel ligands for the benzodiazepine site of alpha5-containing GABAA receptors. | Szekeres HJ et al. | β | 2004 | β |
| 3-phenyl-6-(2-pyridyl)methyloxy-1,2,4-triazolo[3,4-a]phthalazines and analogues: high-affinity gamma-aminobutyric acid-A benzodiazepine receptor ligands with alpha 2, alpha 3, and alpha 5-subtype binding selectivity over alpha 1. | Carling RW et al. | β | 2004 | β |
| Alcoholism: genes and mechanisms. | Oroszi G et al. | β | 2004 | β |
| An orally bioavailable, functionally selective inverse agonist at the benzodiazepine site of GABAA alpha5 receptors with cognition enhancing properties. | Chambers MS et al. | β | 2004 | β |
| Atomic force microscopy and drug discovery. | Edwardson JM et al. | β | 2004 | β |
| Brain regional heterogeneity of pH effects on GABA(A) receptor-associated [35s]TBPS binding. | Uusi-Oukari M et al. | β | 2004 | β |
| Chronic exposure of cells expressing recombinant GABAA receptors to benzodiazepine antagonist flumazenil enhances the maximum number of benzodiazepine binding sites. | PericiΔ D et al. | β | 2004 | β |
| Compounds exhibiting selective efficacy for different beta subunits of human recombinant gamma-aminobutyric acid A receptors. | Smith AJ et al. | β | 2004 | β |
| Functional mapping of GABA A receptor subtypes in the amygdala. | Marowsky A et al. | β | 2004 | β |
| GABAA receptor structure-function studies: a reexamination in light of new acetylcholine receptor structures. | Akabas MH | β | 2004 | β |
| Gating allosterism at a single class of etomidate sites on alpha1beta2gamma2L GABA A receptors accounts for both direct activation and agonist modulation. | RΓΌsch D et al. | β | 2004 | β |
| Genetic manipulations of GABAA receptor in mice make inhibition exciting. | Vicini S et al. | β | 2004 | β |
| Identification of a residue in the gamma-aminobutyric acid type A receptor alpha subunit that differentially affects diazepam-sensitive and -insensitive benzodiazepine site binding. | Derry JM et al. | β | 2004 | β |
| Mutation of glutamate 155 of the GABAA receptor beta2 subunit produces a spontaneously open channel: a trigger for channel activation. | Newell JG et al. | β | 2004 | β |
| On the benzodiazepine binding pocket in GABAA receptors. | Berezhnoy D et al. | β | 2004 | β |
| Positioning of the alpha-subunit isoforms confers a functional signature to gamma-aminobutyric acid type A receptors. | Minier F et al. | β | 2004 | β |
| Regulation of GABAA receptor trafficking, channel activity, and functional plasticity of inhibitory synapses. | LΓΌscher B et al. | β | 2004 | β |
| Salicylidene salicylhydrazide, a selective inhibitor of beta 1-containing GABAA receptors. | Thompson SA et al. | β | 2004 | β |
| Chronic ethanol consumption enhances internalization of alpha1 subunit-containing GABAA receptors in cerebral cortex. | Kumar S et al. | β | 2003 | β |
| Constitutive GABAA receptor endocytosis is dynamin-mediated and dependent on a dileucine AP2 adaptin-binding motif within the beta 2 subunit of the receptor. | Herring D et al. | β | 2003 | β |
| Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9) and in HEK293 cells. Analysis using co-immunoprecipitation and time-resolved fluorescence resonance energy transfer. | Gazi L et al. | β | 2003 | β |
| Formation and plasticity of GABAergic synapses: physiological mechanisms and pathophysiological implications. | Fritschy JM et al. | β | 2003 | β |
| GABA(A) receptor composition is determined by distinct assembly signals within alpha and beta subunits. | Bollan K et al. | β | 2003 | β |
| GABARAP: lessons for synaptogenesis. | Coyle JE et al. | β | 2003 | β |
| Identification of an amino acid sequence within GABA(A) receptor beta3 subunits that is important for receptor assembly. | Ehya N et al. | β | 2003 | β |
| Individual properties of the two functional agonist sites in GABA(A) receptors. | Baumann SW et al. | β | 2003 | β |
| Intact synaptic GABAergic inhibition and altered neurosteroid modulation of thalamic relay neurons in mice lacking delta subunit. | Porcello DM et al. | β | 2003 | β |
| Major differences in inhibitory synaptic transmission onto two neocortical interneuron subclasses. | Bacci A et al. | β | 2003 | β |
| On high- and low-affinity agonist sites in GABAA receptors. | Baur R et al. | β | 2003 | β |
| Zinc-mediated inhibition of GABA(A) receptors: discrete binding sites underlie subtype specificity. | Hosie AM et al. | β | 2003 | β |
| Assembly and cell surface expression of homomeric and heteromeric 5-HT3 receptors: the role of oligomerization and chaperone proteins. | Boyd GW et al. | β | 2002 | β |
| Direct visualization of ligand-protein interactions using atomic force microscopy. | Neish CS et al. | β | 2002 | β |
| Electrophysiological actions of gamma-aminobutyric acid and clomethiazole on recombinant GABA(A) receptors. | Nelson RM et al. | β | 2002 | β |
| Forced subunit assembly in alpha1beta2gamma2 GABAA receptors. Insight into the absolute arrangement. | Baumann SW et al. | β | 2002 | β |
| Functional consequences of the loss of high affinity agonist binding to gamma-aminobutyric acid type A receptors. Implications for receptor desensitization. | Newell JG et al. | β | 2002 | β |
| GABA(A) receptor beta 2 Tyr97 and Leu99 line the GABA-binding site. Insights into mechanisms of agonist and antagonist actions. | Boileau AJ et al. | β | 2002 | β |
| GABAergic innervation organizes synaptic and extrasynaptic GABAA receptor clustering in cultured hippocampal neurons. | Christie SB et al. | β | 2002 | β |
| Glycine and GABA(A) receptor subunits on Renshaw cells: relationship with presynaptic neurotransmitters and postsynaptic gephyrin clusters. | Geiman EJ et al. | β | 2002 | β |
| Homologous sites of GABA(A) receptor alpha(1), beta(3) and gamma(2) subunits are important for assembly. | Sarto I et al. | β | 2002 | β |
| Ivermectin and nodulisporic acid receptors in Drosophila melanogaster contain both gamma-aminobutyric acid-gated Rdl and glutamate-gated GluCl alpha chloride channel subunits. | Ludmerer SW et al. | β | 2002 | β |
| Neonatal development of the rat visual cortex: synaptic function of GABAA receptor alpha subunits. | Bosman LW et al. | β | 2002 | β |
| Overexpression of the GABA(A) receptor epsilon subunit results in insensitivity to anaesthetics. | Thompson SA et al. | β | 2002 | β |
| Principles and biophysical applications of lanthanide-based probes. | Selvin PR | β | 2002 | β |
| Structure of GABARAP in two conformations: implications for GABA(A) receptor localization and tubulin binding. | Coyle JE et al. | β | 2002 | β |
| The relative amount of cRNA coding for gamma2 subunits affects stimulation by benzodiazepines in GABA(A) receptors expressed in Xenopus oocytes. | Boileau AJ et al. | β | 2002 | β |
| Time resolved amplification of cryptate emission: a versatile technology to trace biomolecular interactions. | Bazin H et al. | β | 2002 | β |
| Tracazolate reveals a novel type of allosteric interaction with recombinant gamma-aminobutyric acid(A) receptors. | Thompson SA et al. | β | 2002 | β |
| Tryptophan scanning mutagenesis in TM4 of the GABA(A) receptor alpha1 subunit: implications for modulation by inhaled anesthetics and ion channel structure. | Jenkins A et al. | β | 2002 | β |
| alpha4beta3delta GABA(A) receptors characterized by fluorescence resonance energy transfer-derived measurements of membrane potential. | Adkins CE et al. | β | 2001 | β |
| Constructing inhibitory synapses. | Moss SJ et al. | β | 2001 | β |
| Detection and binding properties of GABA(A) receptor assembly intermediates. | Klausberger T et al. | β | 2001 | β |
| Evidence for distinct conformations of the two alpha 1 subunits in diazepam-bound GABA(A) receptors. | Williams DB et al. | β | 2001 | β |
| Evidence for the formation of functionally distinct alphabetagammaepsilon GABA(A) receptors. | Davies PA et al. | β | 2001 | β |
| GABA(A) receptor epsilon-subunit may confer benzodiazepine insensitivity to the caudal aspect of the nucleus tractus solitarii of the rat. | Kasparov S et al. | β | 2001 | β |
| Homogeneous time resolved fluorescence resonance energy transfer using rare earth cryptates as a tool for probing molecular interactions in biology. | Bazin H et al. | β | 2001 | β |
| Molecular determinants of desensitization and assembly of the chimeric GABA(A) receptor subunits (alpha1/gamma2) and (gamma2/alpha1) in combinations with beta2 and gamma2. | Elster L et al. | β | 2001 | β |
| Monitoring receptor oligomerization using time-resolved fluorescence resonance energy transfer and bioluminescence resonance energy transfer. The human delta -opioid receptor displays constitutive oligomerization at the cell surface, which is not regulated by receptor occupancy. | McVey M et al. | β | 2001 | β |
| Prevalence between different alpha subunits performing the benzodiazepine binding sites in native heterologous GABA(A) receptors containing the alpha2 subunit. | del RΓo JC et al. | β | 2001 | β |
| Protein mobility and GABA-induced conformational changes in GABA(A) receptor pore-lining M2 segment. | Horenstein J et al. | β | 2001 | β |
| The non-peptidyl fungal metabolite L-783,281 activates TRK neurotrophin receptors. | Wilkie N et al. | β | 2001 | β |
| Benzodiazepines induce a conformational change in the region of the gamma-aminobutyric acid type A receptor alpha(1)-subunit M3 membrane-spanning segment. | Williams DB et al. | β | 2000 | β |
| Development of a time-resolved fluorometric detection system using diffusion-enhanced energy transfer | Koresawa M et al. | β | 2000 | β |
| Fluorescence properties of pyrylretinol. | Das J et al. | β | 2000 | β |
| GABA potentiation: a logical pharmacological approach for the treatment of acute ischaemic stroke. | Green AR et al. | β | 2000 | β |
| Influence of subunit configuration on the interaction of picrotoxin-site ligands with recombinant GABA(A) receptors. | Bell-Horner CL et al. | β | 2000 | β |
| N-(indol-3-ylglyoxylyl)piperidines: high affinity agonists of human GABA-A receptors containing the alpha1 subunit. | Collins I et al. | β | 2000 | β |
| The actions of ether, alcohol and alkane general anaesthetics on GABAA and glycine receptors and the effects of TM2 and TM3 mutations. | Krasowski MD et al. | β | 2000 | β |
| Tryptophan scanning mutagenesis in TM2 of the GABA(A) receptor alpha subunit: effects on channel gating and regulation by ethanol. | Ueno S et al. | β | 2000 | β |
| Two conserved arginines in the extracellular N-terminal domain of the GABA(A) receptor alpha(5) subunit are crucial for receptor function. | Hartvig L et al. | β | 2000 | β |
| Tyrosine 62 of the gamma-aminobutyric acid type A receptor beta 2 subunit is an important determinant of high affinity agonist binding. | Newell JG et al. | β | 2000 | β |
| Gamma-aminobutyric acid increases the water accessibility of M3 membrane-spanning segment residues in gamma-aminobutyric acid type A receptors. | Williams DB et al. | β | 1999 | β |