Participation of endocannabinoids in rapid suppression of stress responses by glucocorticoids in neonates.
- Authors
- Buwembo, A; Long, H; Walker, C-D
- Year
- 2013
- Journal
- Neuroscience
- PMID
- 23131711
- DOI
- 10.1016/j.neuroscience.2012.10.057
In adult rodents, endocannabinoids (eCBs) regulate fast glucocorticoid (GC) feedback in the hypothalamus-pituitary adrenal (HPA) axis, acting as retrograde messengers that bind to cannabinoid receptors (CB1R) and inhibit glutamate release from presynaptic CRH neurons in the paraventricular nucleus of the hypothalamus (PVN). During the first two weeks of life, rat pups exhibit significant CRH and ACTH responses to stress although the adrenal GC output remains reduced. At the same time, pups also display increased sensitivity to GC feedback, but it is unclear whether eCBs play a role in mediating fast GC feedback in neonatal life. In our studies, we examined the role of eCBs in the rapid suppression of anoxia-induced ACTH release and determined whether eCB action could be modulated by the levels of circulating GCs present at the time of stress. PND8 pups were subjected to 3-min anoxia with AM251, a CB1R blocker, injected 30 min prior to stress onset. The effects of either metyrapone (MET) (a steroidogenic 11 beta-hydroxylase blocker) or methylprednisolone (PRED) (a synthetic GC) pretreatment on AM251 effect and the stress response were evaluated. Treatment with AM251 before stress onset tended to increase overall ACTH and CORT secretion, and also delayed the return to baseline ACTH. The AM251 effect on ACTH in PND8 pups was lost in MET-treated pups, who exhibited high basal and stimulated ACTH release and no CORT response to stress. Methylprednisolone suppressed ACTH stress responses although AM251 still delayed restoration of ACTH levels to the baseline. This suggests that the eCB effect on ACTH secretion in neonates is most evident when there is a dynamic fluctuation of corticosterone levels. Interestingly, AM251 increased basal and stimulated corticosterone secretion in all treatments including MET, suggestive of a direct action of CB1R blockade on adrenal steroidogenesis.
No figures extracted from this document.
No chunks — full text not yet ingested.
No entities extracted from this document yet.
No uploaded files.
No citations found.
In this knowledge base
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Corticosterone effects induced by stress and immunity and inflammation: mechanisms of communication. | Xu J et al. | — | 2025 | → |
| Dynamic effects of chronic unpredictable mild stress on the hippocampal transcriptome in rats. | Li F et al. | — | 2022 | → |
| Memory Consolidation Depends on Endogenous Hippocampal Levels of Anandamide: CB1 and M4, but Possibly not TRPV1 Receptors Mediate AM404 effects. | Scienza-Martin K et al. | — | 2022 | → |
| Early life stress and development of the endocannabinoid system: A bidirectional process in programming future coping. | Goldstein Ferber S et al. | — | 2021 | → |
| Is PTSD-Phenotype Associated with HPA-Axis Sensitivity?: The Endocannabinoid System in Modulating Stress Response in Rats. | Danan D et al. | — | 2021 | → |
| Prenatal tobacco and marijuana co-use: Sex-specific influences on infant cortisol stress response. | Stroud LR et al. | — | 2020 | → |
| Early life stress alters the developmental trajectory of corticolimbic endocannabinoid signaling in male rats. | Hill MN et al. | — | 2019 | → |
| Endocannabinoids in brain plasticity: Cortical maturation, HPA axis function and behavior. | Dow-Edwards D et al. | — | 2017 | → |
| Endocannabinoids, through opioids and prostaglandins, contribute to fever induced by key pyrogenic mediators. | Fraga D et al. | — | 2016 | → |
| Genetic and Environmental Factors Associated with Cannabis Involvement. | Bogdan R et al. | — | 2016 | → |
| Inhibition of anandamide hydrolysis dampens the neuroendocrine response to stress in neonatal rats subjected to suboptimal rearing conditions. | McLaughlin RJ et al. | — | 2016 | → |
| Monoacylglycerol lipase (MGLL) polymorphism rs604300 interacts with childhood adversity to predict cannabis dependence symptoms and amygdala habituation: Evidence from an endocannabinoid system-level analysis. | Carey CE et al. | — | 2015 | → |
| The consequences of pain in early life: injury-induced plasticity in developing pain pathways. | Schwaller F et al. | — | 2014 | → |