Chronic ethanol consumption enhances internalization of alpha1 subunit-containing GABAA receptors in cerebral cortex.
paper
Cited
Public
Unavailable
- Authors
- Kumar, S; Kralic, J E; O'Buckley, T K; Grobin, A C; Morrow, A L
- Year
- 2003
- Journal
- Journal of neurochemistry
- PMID
- 12859683
- DOI
- 10.1046/j.1471-4159.2003.01894.x
The molecular mechanisms that underlie ethanol dependence involve alterations in the functional properties and subunit expression of GABAA receptors. Chronic ethanol exposure decreases GABAA receptor alpha1 subunits and increases alpha4 subunit levels in cerebral cortical membranes. This study explored the effect of chronic ethanol exposure on internalization of GABAA/benzodiazepine receptors. Chronic ethanol exposure increased alpha1 subunit levels by 46 +/- 12% and [3H]flunitrazepam binding by 35 +/- 9% in the clathrin-coated vesicle (CCV) fraction. There was a corresponding 34 +/- 8% decrease in alpha1 peptide expression and 37 +/- 6% decrease in [3H]flunitrazepam binding in the synaptic fraction. Chronic ethanol consumption also increased the alpha1 subunit immunoprecipitate in the cytosolic fraction (77 +/- 22%), measured by western blot analysis. Moreover, co-immunoprecipitation of both clathrin and adaptin-alpha with alpha1 subunits was increased in the cytosolic fraction, suggesting that alpha1 subunit endocytosis is enhanced by chronic ethanol consumption. In contrast, alpha4 subunit peptide levels were not altered in the CCV fraction despite a 39 +/- 13% increase in peptide levels in the synaptic fraction of cortex. Moreover, acute ethanol exposure did not alter alpha1 subunit peptide expression or [3H]flunitrazepam binding in the synaptic or CCV fractions. These results suggest that chronic ethanol consumption selectively increases internalization of alpha1 subunit-containing GABAA receptors in cerebral cortex.
No figures extracted from this document.
No chunks β full text not yet ingested.
No entities extracted from this document yet.
No uploaded files.
Not in any collection.
No citations found.
In this knowledge base
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| Modulatory effects of GLT-1 enhancer, MC-100093, on neuroinflammatory factors in mesocorticolimbic brain regions of female P rats exposed chronically to ethanol. | Bhowmik KK et al. | β | 2026 | β |
| Multi-omics and electrophysiological examination of GABA<sub>A</sub> receptors in the dorsolateral prefrontal cortex of humans with alcohol use disorder. | Granchi J et al. | β | 2026 | β |
| Altered <i>glr-1</i> expression is linked to EtOH cessation-mediated behavior deficits in <i>C. elegans</i> | Brandel-Ankrapp K et al. | β | 2025 | β |
| Promising strategies for the prevention of alcohol-related brain damage through optimised management of acute alcohol withdrawal: A focussed literature review. | Quelch D et al. | β | 2025 | β |
| Synaptic Effects Induced by Alcohol. | Lovinger DM et al. | β | 2025 | β |
| In the fast lane: Receptor trafficking during status epilepticus. | Naylor DE | β | 2023 | β |
| Neurosteroids (allopregnanolone) and alcohol use disorder: From mechanisms to potential pharmacotherapy. | Gatta E et al. | β | 2022 | β |
| Prospective DNA Methylation Analysis of the CpG GABRA2 Receptor Subunit in Alcohol Dependence during Detoxification. | Preuss UW et al. | β | 2022 | β |
| Sex differences in GABA<sub>A</sub> receptor subunit transcript expression are mediated by genotype in subjects with alcohol-related cirrhosis of the liver. | Ashton MK et al. | β | 2022 | β |
| Correlations between subunits of GABA<sub>A</sub> and NMDA receptors after chronic alcohol treatment or withdrawal, and the effect of taurine in the hippocampus of rats. | Hansen AW et al. | β | 2020 | β |
| Regulation of GABA<sub>A</sub> Receptor Subunit Expression in Substance Use Disorders. | Barker JS et al. | β | 2020 | β |
| Chronic Ethanol Exposure and Withdrawal Impair Synaptic GABA<sub>A</sub> Receptor-Mediated Neurotransmission in Deep-Layer Prefrontal Cortex. | Hughes BA et al. | β | 2019 | β |
| Ethanol actions on the ventral tegmental area: novel potential targets on reward pathway neurons. | You C et al. | β | 2018 | β |
| Regulation of GABA<sub>A</sub> receptors by prolonged exposure to endogenous and exogenous ligands. | Gravielle MC | β | 2018 | β |
| Role of GABA<sub>A</sub> receptors in alcohol use disorders suggested by chronic intermittent ethanol (CIE) rodent model. | Olsen RW et al. | β | 2017 | β |
| Synaptic targets: Chronic alcohol actions. | Roberto M et al. | β | 2017 | β |
| Ethanol Regulation of Synaptic GABAA Ξ±4 Receptors Is Prevented by Protein Kinase A Activation. | Carlson SL et al. | β | 2016 | β |
| Regulation of Extrasynaptic GABAA Ξ±4 Receptors by Ethanol-Induced Protein Kinase A, but Not Protein Kinase C Activation in Cultured Rat Cerebral Cortical Neurons. | Carlson SL et al. | β | 2016 | β |
| Thinking after Drinking: Impaired Hippocampal-Dependent Cognition in Human Alcoholics and Animal Models of Alcohol Dependence. | Staples MC et al. | β | 2016 | β |
| Adolescent rats are resistant to the development of ethanol-induced chronic tolerance and ethanol-induced conditioned aversion. | Pautassi RM et al. | β | 2015 | β |
| Exogenous progesterone exacerbates running response of adolescent female mice to repeated food restriction stress by changing Ξ±4-GABAA receptor activity of hippocampal pyramidal cells. | Wable GS et al. | β | 2015 | β |
| Regulation of GABAARs by phosphorylation. | Nakamura Y et al. | β | 2015 | β |
| Alcohol use disorders and current pharmacological therapies: the role of GABA(A) receptors. | Liang J et al. | β | 2014 | β |
| Ethanol-induced plasticity of GABAA receptors in the basolateral amygdala. | Lindemeyer AK et al. | β | 2014 | β |
| Ethanol activation of protein kinase A regulates GABAA Ξ±1 receptor function and trafficking in cultured cerebral cortical neurons. | Carlson SL et al. | β | 2013 | β |
| Hyposensitivity to gamma-aminobutyric acid in the ventral tegmental area during alcohol withdrawal: reversal by histone deacetylase inhibitors. | Arora DS et al. | β | 2013 | β |
| Signaling pathways mediating alcohol effects. | Ron D et al. | β | 2013 | β |
| Synaptic effects induced by alcohol. | Lovinger DM et al. | β | 2013 | β |
| The impairing effect of acute ethanol on spreading depression is antagonized by astaxanthin in rats of 2 young-adult ages. | Abadie-Guedes R et al. | β | 2012 | β |
| Effect of acute soman exposure on GABA(A) receptors in rat hippocampal slices and cultured hippocampal neurons. | Wang Y et al. | β | 2011 | β |
| Neuroimaging insights into the role of cortical GABA systems and the influence of nicotine on the recovery from alcohol dependence. | Cosgrove KP et al. | β | 2011 | β |
| Ethanol reduces GABAA alpha1 subunit receptor surface expression by a protein kinase Cgamma-dependent mechanism in cultured cerebral cortical neurons. | Kumar S et al. | β | 2010 | β |
| Ethanol self-administration modulation of NMDA receptor subunit and related synaptic protein mRNA expression in prefrontal cortical fields in cynomolgus monkeys. | Acosta G et al. | β | 2010 | β |
| Wnt-5a modulates recycling of functional GABAA receptors on hippocampal neurons. | Cuitino L et al. | β | 2010 | β |
| Abuse Potential of Soma: the GABA(A) Receptor as a Target. | Gonzalez LA et al. | β | 2009 | β |
| Alcohol-induced tolerance and physical dependence in mice with ethanol insensitive alpha1 GABA A receptors. | Werner DF et al. | β | 2009 | β |
| Altering the relative abundance of GABA A receptor subunits changes GABA- and ethanol-responses in Xenopus oocytes. | Hurley JH et al. | β | 2009 | β |
| Expression levels of the alpha4 subunit of the GABA(A) receptor in differentiated neuroblastoma cells are correlated with GABA-gated current. | Zhou X et al. | β | 2009 | β |
| The effects of acute and chronic ethanol exposure on presynaptic and postsynaptic gamma-aminobutyric acid (GABA) neurotransmission in cultured cortical and hippocampal neurons. | Fleming RL et al. | β | 2009 | β |
| The role of GABA(A) receptors in the acute and chronic effects of ethanol: a decade of progress. | Kumar S et al. | β | 2009 | β |
| Dose-dependent effects of astaxanthin on cortical spreading depression in chronically ethanol-treated adult rats. | Abadie-Guedes R et al. | β | 2008 | β |
| Expression of GABA A receptor alpha1 subunit mRNA and protein in rat neocortex following photothrombotic infarction. | Kharlamov EA et al. | β | 2008 | β |
| GABA(A) receptor trafficking and its role in the dynamic modulation of neuronal inhibition. | Jacob TC et al. | β | 2008 | β |
| The molecular basis of tolerance. | Pietrzykowski AZ et al. | β | 2008 | β |
| The role of GABA(A) receptors in the development of alcoholism. | Enoch MA | β | 2008 | β |
| Effect of chronic administration of ethanol on the regulation of the delta-subunit of GABA(A) receptors in the rat brain. | Marutha Ravindran CR et al. | β | 2007 | β |
| Mechanisms of reversible GABAA receptor plasticity after ethanol intoxication. | Liang J et al. | β | 2007 | β |
| Neurosteroid regulation of GABA(A) receptors: Focus on the alpha4 and delta subunits. | Smith SS et al. | β | 2007 | β |
| Chronic ethanol drinking reduces native T-type calcium current in the thalamus of nonhuman primates. | Carden WB et al. | β | 2006 | β |
| Differential effects of systemic ethanol administration on protein kinase cepsilon, gamma, and beta isoform expression, membrane translocation, and target phosphorylation: reversal by chronic ethanol exposure. | Kumar S et al. | β | 2006 | β |
| Ethanol-induced regulation of GABA-A subunit mRNAs in prefrontal fields of cynomolgus monkeys. | Hemby SE et al. | β | 2006 | β |
| Ethanol inhibits L1 cell adhesion molecule activation of mitogen-activated protein kinases. | Tang N et al. | β | 2006 | β |
| Ethanol tolerance caused by slowpoke induction in Drosophila. | Cowmeadow RB et al. | β | 2006 | β |
| GABAA receptor regulation of voluntary ethanol drinking requires PKCepsilon. | Besheer J et al. | β | 2006 | β |
| GABAergic phthalide dimers from Angelica sinensis (Oliv.) Diels. | Deng S et al. | β | 2006 | β |
| Running to stand still: ionotropic receptor dynamics at central and peripheral synapses. | Bruneau EG et al. | β | 2006 | β |
| Effect of chronic administration of ethanol on GABAA receptor assemblies derived from alpha2-, alpha3-, beta2- and gamma2-subunits in the rat cerebral cortex. | Mehta AK et al. | β | 2005 | β |
| GABA(A) receptor subunit expression in the guinea pig vestibular nucleus complex during the development of vestibular compensation. | Gliddon CM et al. | β | 2005 | β |
| Internalized GABA-receptor subunits are transferred to an intracellular pool associated with the postsynaptic density. | van Rijnsoever C et al. | β | 2005 | β |
| Phospho-dependent binding of the clathrin AP2 adaptor complex to GABAA receptors regulates the efficacy of inhibitory synaptic transmission. | Kittler JT et al. | β | 2005 | β |
| Short-term steroid treatment increases delta GABAA receptor subunit expression in rat CA1 hippocampus: pharmacological and behavioral effects. | Shen H et al. | β | 2005 | β |
| Trafficking of GABA(A) receptors, loss of inhibition, and a mechanism for pharmacoresistance in status epilepticus. | Naylor DE et al. | β | 2005 | β |
| Update on the neurobiology of alcohol withdrawal seizures. | Rogawski MA | β | 2005 | β |
| Alcoholism: genes and mechanisms. | Oroszi G et al. | β | 2004 | β |
| Alcohol tolerance in large-conductance, calcium-activated potassium channels of CNS terminals is intrinsic and includes two components: decreased ethanol potentiation and decreased channel density. | Pietrzykowski AZ et al. | β | 2004 | β |
| Altered pharmacology of synaptic and extrasynaptic GABAA receptors on CA1 hippocampal neurons is consistent with subunit changes in a model of alcohol withdrawal and dependence. | Liang J et al. | β | 2004 | β |
| Binge ethanol exposure delays development of GABAergic miniature postsynaptic currents in septal neurons. | DuBois DW et al. | β | 2004 | β |
| Differential effects of chronic ethanol administration and withdrawal on gamma-aminobutyric acid type A and NMDA receptor subunit proteins in male and female rat brain. | Devaud LL et al. | β | 2004 | β |
| Ethanol effects on cell signaling mechanisms. | Morrow AL et al. | β | 2004 | β |
| Ethanol regulation of gamma-aminobutyric acid A receptors: genomic and nongenomic mechanisms. | Kumar S et al. | β | 2004 | β |
| GABAA receptors: building the bridge between subunit mRNAs, their promoters, and cognate transcription factors. | Steiger JL et al. | β | 2004 | β |
| Genetic manipulations of GABAA receptor in mice make inhibition exciting. | Vicini S et al. | β | 2004 | β |
| Hippocampal and striated skeletal muscle changes in fatty acid composition induced by ethanol in alcohol-preferring rats. | Berrettini M et al. | β | 2004 | β |
| Long-term ethanol self-administration by cynomolgus macaques alters the pharmacology and expression of GABAA receptors in basolateral amygdala. | Floyd DW et al. | β | 2004 | β |
| The cerebellar GABAA alpha6 subunit is differentially modulated by chronic ethanol exposure in normal (R100R) and mutated (Q100Q) sNP rats. | Sanna A et al. | β | 2004 | β |
| Changes in GABA(A) receptor gene expression associated with selective alterations in receptor function and pharmacology after ethanol withdrawal. | Sanna E et al. | β | 2003 | β |