Effect of polymorphism on expression of the neuropeptide Y gene in inbred alcohol-preferring and -nonpreferring rats.
- Authors
- Spence, J P; Liang, T; Habegger, K; Carr, L G
- Year
- 2005
- Journal
- Neuroscience
- PMID
- 15749341
- DOI
- 10.1016/j.neuroscience.2004.10.013
- PMCID
- PMC4455873
Using animal models of alcoholism, previous studies suggest that neuropeptide Y (NPY) may be implicated in alcohol preference and consumption due to its role in the modulation of feeding and anxiety. Quantitative trait loci (QTL) analysis previously identified an interval on rat chromosome 4 that is highly associated with alcohol preference and consumption using an F2 population derived from inbred alcohol-preferring (iP) and -nonpreferring (iNP) rats. NPY mapped to the peak of this QTL region and was prioritized as a candidate gene for alcohol-seeking behavior in the iP and iNP rats. In order to identify a potential mechanism for reduced NPY protein levels documented in the iP rat, genetic and molecular components that influence NPY expression were analyzed between iP and iNP rats. Comparing the iP rat to the iNP rat, quantitative real-time polymerase chain reaction detected significantly decreased levels of NPY mRNA expression in the iP rat in the six brain regions tested: nucleus accumbens, frontal cortex, amygdala, hippocampus, caudate-putamen, and hypothalamus. In addition, the functional significance of three previously identified polymorphisms was assessed using in vitro expression analysis. The polymorphism defined by microsatellite marker D4Mit7 in iP rats reduced luciferase reporter gene expression in SK-N-SH neuroblastoma cells. These results suggest that differential expression of the NPY gene resulting from the D4mit7 marker polymorphism may contribute to reduced levels of NPY in discrete brain regions in the iP rats.
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In this knowledge base
| Title | Year | PMID |
|---|---|---|
| Neuropeptide Y receptor genes are associated with alcohol dependence, alcohol withdrawal phenotypes, and cocaine dependence. | 2008 | 18828811 |
External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
| A Genetic Animal Model of Alcoholism for Screening Medications to Treat Addiction. | Bell RL et al. | — | 2016 | → |
| Hypothalamic neuropeptide signaling in alcohol addiction. | Barson JR et al. | — | 2016 | → |
| Npy deletion in an alcohol non-preferring rat model elicits differential effects on alcohol consumption and body weight. | Qiu B et al. | — | 2016 | → |
| Genetics of alcohol dependence: a review of clinical studies. | Samochowiec J et al. | — | 2014 | → |
| Association between neuropeptide Y gene polymorphisms and alcohol dependence: a case-control study in two independent populations. | Bhaskar LV et al. | — | 2013 | → |
| Quantitative trait locus for body weight identified on rat chromosome 4 in inbred alcohol-preferring and -nonpreferring rats: potential implications for neuropeptide Y and corticotrophin releasing hormone 2. | Spence JP et al. | — | 2013 | → |
| Candidate genes for alcohol preference identified by expression profiling in alcohol-preferring and -nonpreferring reciprocal congenic rats. | Liang T et al. | — | 2010 | → |
| Functional NPY variation as a factor in stress resilience and alcohol consumption in rhesus macaques. | Lindell SG et al. | — | 2010 | → |
| Genetic variation and brain gene expression in rodent models of alcoholism implications for medication development. | Björk K et al. | — | 2010 | → |
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| Neuropeptide Y signaling in the central nucleus of amygdala regulates alcohol-drinking and anxiety-like behaviors of alcohol-preferring rats. | Zhang H et al. | — | 2010 | → |
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| New findings on the genetic influences on alcohol use and dependence. | Higuchi S et al. | — | 2006 | → |