High expression and prognostic role of CAP1 and CtBP2 in breast carcinoma: associated with E-cadherin and cell proliferation.
- Authors
- Liu, Xiancheng; Yao, Ninghua; Qian, Jing; Huang, Huiwei
- Year
- 2014
- Journal
- Medical oncology (Northwood, London, England)
- PMID
- 24522810
- DOI
- 10.1007/s12032-014-0878-7
Overexpression of C-terminal binding protein-2 (CtBP2) has been noted to correlate with cancer metastasis in several human cancers including breast cancer. The aim of this study was to examine the effect of cyclase-associated protein 1 (CAP1) overexpression on CtBP2 expression and related mechanism in the metastasis of breast cancer. Immunohistochemical analysis was performed in 100 human breast carcinoma samples, and the data were correlated with clinicopathologic features. Furthermore, Western blot analysis was performed for CAP1 and CtBP2 in breast carcinoma samples and cell lines to evaluate their protein levels and molecular interaction. We found that the expression of CAP1 was positively related to CtBP2 expression (P<0.01); moreover, CAP1 expression was significantly correlated with histologic grade (P<0.01) and negatively related to E-cadherin expression (P<0.01). Meanwhile, CtBP2 expression obtained similar results. Kaplan-Meier survival analysis showed that overexpression of CAP1 and CtBP2 exhibited a significant correlation with poor prognosis in human breast cancer (P<0.01). While in vitro, we employed siRNA technique to knockdown CAP1 and CtBP2 expressions and observed their effects on MDA-MB-231 cells growth. CtBP2 depletion by siRNA-inhibited cell proliferation, resulted in increased E-cadherin levels. Moreover, knockdown of CAP1 resulted in decreased CtBP2 and increased E-cadherin expression. On the basis of these results, we suggested that CAP1's oncogenic abilities appear to be triggered at least in part by the modulation of CtBP2 and E-cadherin, which might serve as a future target for breast cancer.
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External
| Title | Authors | Journal | Year | Link |
|---|---|---|---|---|
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| Adenylyl Cyclase-Associated Protein 1: Structure, Regulation, and Participation in Cellular Processes. | Kakurina GV et al. | — | 2018 | → |
| Adipocytes and Obesity-Related Conditions Jointly Promote Breast Cancer Cell Growth and Motility: Associations With CAP1 for Prognosis. | Rosendahl AH et al. | — | 2018 | → |
| Cyclase-associated protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase. | Zhang X et al. | — | 2018 | → |
| Adiponectin and Intelectin-1: Important Adipokine Players in Obesity-Related Colorectal Carcinogenesis. | Kawashima K et al. | — | 2017 | → |
| A genome wide association study of fast beta EEG in families of European ancestry. | Meyers JL et al. | — | 2017 | → |
| CtBP2 overexpression promotes tumor cell proliferation and invasion in gastric cancer and is associated with poor prognosis. | Dai F et al. | — | 2017 | → |
| C-terminal binding protein-2 promotes cell proliferation and migration in breast cancer via suppression of p16INK4A. | Yang X et al. | — | 2017 | → |
| Prognostic value of reduced E-cadherin expression in breast cancer: a meta-analysis. | Li Z et al. | — | 2017 | → |
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| High expression of adenylate cyclase-associated protein 1 accelerates the proliferation, migration and invasion of neural glioma cells. | Bao Z et al. | — | 2016 | → |
| Multivariate models from RNA-Seq SNVs yield candidate molecular targets for biomarker discovery: SNV-DA. | Paul MR et al. | — | 2016 | → |
| CtBP2 is an independent prognostic marker that promotes GLI1 induced epithelial-mesenchymal transition in hepatocellular carcinoma. | Zheng X et al. | — | 2015 | → |
| Increased Expression of CAP2 Indicates Poor Prognosis in Hepatocellular Carcinoma. | Fu J et al. | — | 2015 | → |
| Overexpression of adenylate cyclase-associated protein 2 is a novel prognostic marker in malignant melanoma. | Masugi Y et al. | — | 2015 | → |
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